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The map label for this gene is dfrA [H]

Identifier: 114797778

GI number: 114797778

Start: 3506694

End: 3507716

Strand: Direct

Name: dfrA [H]

Synonym: HNE_3360

Alternate gene names: 114797778

Gene position: 3506694-3507716 (Clockwise)

Preceding gene: 114800316

Following gene: 114797311

Centisome position: 94.65

GC content: 66.28

Gene sequence:

>1023_bases
ATGGCGAAGGTTCTTGTCACAGGCGCAACCGGCTTCATCGCCGGGCATGTCATCCACCAGCTGCTCGAAGCGGGCCATGA
GGTGATCGGAACGGCCCGTTCCGCCTCAAAGGCCGGGCCGCTGAACCAGACCCTCAGCGCCTATGCCGGCCGGCCGGTCT
CAATCGAAATCCGCGCCGCAGACCTCTCCGCCGATGCAGGCTGGACAGAGGCCGTGGCGGGGGTCGACGTCGTCCAGCAT
GTCGCCTCCCCCATTCCGCTGACCGTGCCGAAGAATGCCGATGAGCTGATCATTCCCGCCCGCGACGGCGCGCTACGTGT
GCTGAGGGCGGCCAAGGCCGCGGGCGTCAGGCGCGTGGTGATGACCTCCTCGATGGCGGCCTGTGCCTATGGCTGGGGCG
AGGCGCGCCCCAACCCGATCACCGAGGAACACTGGTCGCAGGACAATGGCGCGGCGGACATGACCCCCTACATCCGCTCC
AAGCTGATCGCCGAGCGGGCGGCATGGGATTATGTCAGCGATGAAGGCAAGGGCCTGGCGCTGACCACCATCAACCCGGC
CCTCGTGCTCGGCCCGGTGATGAGCGGGGACTTTTCCGCCTCGGTCGAAATCCTCACCCAGCTGATGTCGGGCAAGCTGC
CGGGCACGCCGAAGGTGGGCTTCGTGGTGGTGGATGTGCGCGATGTGGCGGCTGCCCATGTGGCCGCGATGACGAACCCG
GCGGCCGAAGGCGAACGCTTCCTGCTGGGCGACCGGTTCATCTGGTTCCGCGACGTGGCCGAAGTGCTGCGCCGCGAGTT
TCCAGACTATGCCTCAAAAGTTCCGTCCAGGGACATTCCCGGCTGGATGGTCCACCTGATCGCCCTGATCAACCCACCCG
CCAAACAGCTCATCCCCGAACTCAGCCGCGAACGGCATATCTCCAGCGAGAAGGCGCGGCGCGTTCTGGACTGGACCCCG
CGCAGCAGCGAAGAAGCTGTGATCGCAGGCGCGCGGAGCCTGATTGGGTTTGGGGTGGTTTGA

Upstream 100 bases:

>100_bases
ATCTCAGGACACTTTGAGACAAGGTGAGACGCCCTTCCCCAGATTCCCCTTGTGAACGAAAAACGTTTCGTTTATTGTTC
CACAAAATCGGGAGGACGGT

Downstream 100 bases:

>100_bases
GGGGGGCGATTCCCGTGCTCTCAATCCTATAAGCGGCGGGTTTGTTTAGGTTTTAATCTAAAAAATCATCGCGTTCCCTG
GCTAAAGTTTGCTTGCAAAG

Product: NAD-dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAKVLVTGATGFIAGHVIHQLLEAGHEVIGTARSASKAGPLNQTLSAYAGRPVSIEIRAADLSADAGWTEAVAGVDVVQH
VASPIPLTVPKNADELIIPARDGALRVLRAAKAAGVRRVVMTSSMAACAYGWGEARPNPITEEHWSQDNGAADMTPYIRS
KLIAERAAWDYVSDEGKGLALTTINPALVLGPVMSGDFSASVEILTQLMSGKLPGTPKVGFVVVDVRDVAAAHVAAMTNP
AAEGERFLLGDRFIWFRDVAEVLRREFPDYASKVPSRDIPGWMVHLIALINPPAKQLIPELSRERHISSEKARRVLDWTP
RSSEEAVIAGARSLIGFGVV

Sequences:

>Translated_340_residues
MAKVLVTGATGFIAGHVIHQLLEAGHEVIGTARSASKAGPLNQTLSAYAGRPVSIEIRAADLSADAGWTEAVAGVDVVQH
VASPIPLTVPKNADELIIPARDGALRVLRAAKAAGVRRVVMTSSMAACAYGWGEARPNPITEEHWSQDNGAADMTPYIRS
KLIAERAAWDYVSDEGKGLALTTINPALVLGPVMSGDFSASVEILTQLMSGKLPGTPKVGFVVVDVRDVAAAHVAAMTNP
AAEGERFLLGDRFIWFRDVAEVLRREFPDYASKVPSRDIPGWMVHLIALINPPAKQLIPELSRERHISSEKARRVLDWTP
RSSEEAVIAGARSLIGFGVV
>Mature_339_residues
AKVLVTGATGFIAGHVIHQLLEAGHEVIGTARSASKAGPLNQTLSAYAGRPVSIEIRAADLSADAGWTEAVAGVDVVQHV
ASPIPLTVPKNADELIIPARDGALRVLRAAKAAGVRRVVMTSSMAACAYGWGEARPNPITEEHWSQDNGAADMTPYIRSK
LIAERAAWDYVSDEGKGLALTTINPALVLGPVMSGDFSASVEILTQLMSGKLPGTPKVGFVVVDVRDVAAAHVAAMTNPA
AEGERFLLGDRFIWFRDVAEVLRREFPDYASKVPSRDIPGWMVHLIALINPPAKQLIPELSRERHISSEKARRVLDWTPR
SSEEAVIAGARSLIGFGVV

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=348, Percent_Identity=39.367816091954, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321399, Length=348, Percent_Identity=31.3218390804598, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321281, Length=295, Percent_Identity=32.2033898305085, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320750, Length=293, Percent_Identity=31.0580204778157, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324421, Length=294, Percent_Identity=30.952380952381, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36285; Mature: 36154

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLVTGATGFIAGHVIHQLLEAGHEVIGTARSASKAGPLNQTLSAYAGRPVSIEIRAA
CCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEE
DLSADAGWTEAVAGVDVVQHVASPIPLTVPKNADELIIPARDGALRVLRAAKAAGVRRVV
CCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
MTSSMAACAYGWGEARPNPITEEHWSQDNGAADMTPYIRSKLIAERAAWDYVSDEGKGLA
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LTTINPALVLGPVMSGDFSASVEILTQLMSGKLPGTPKVGFVVVDVRDVAAAHVAAMTNP
EEECCCHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC
AAEGERFLLGDRFIWFRDVAEVLRREFPDYASKVPSRDIPGWMVHLIALINPPAKQLIPE
CCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHH
LSRERHISSEKARRVLDWTPRSSEEAVIAGARSLIGFGVV
HHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKVLVTGATGFIAGHVIHQLLEAGHEVIGTARSASKAGPLNQTLSAYAGRPVSIEIRAA
CEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEE
DLSADAGWTEAVAGVDVVQHVASPIPLTVPKNADELIIPARDGALRVLRAAKAAGVRRVV
CCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
MTSSMAACAYGWGEARPNPITEEHWSQDNGAADMTPYIRSKLIAERAAWDYVSDEGKGLA
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LTTINPALVLGPVMSGDFSASVEILTQLMSGKLPGTPKVGFVVVDVRDVAAAHVAAMTNP
EEECCCHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC
AAEGERFLLGDRFIWFRDVAEVLRREFPDYASKVPSRDIPGWMVHLIALINPPAKQLIPE
CCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHH
LSRERHISSEKARRVLDWTPRSSEEAVIAGARSLIGFGVV
HHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]