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The map label for this gene is yngF [H]

Identifier: 114797752

GI number: 114797752

Start: 2093046

End: 2093831

Strand: Direct

Name: yngF [H]

Synonym: HNE_2016

Alternate gene names: 114797752

Gene position: 2093046-2093831 (Clockwise)

Preceding gene: 114797375

Following gene: 114798844

Centisome position: 56.49

GC content: 65.52

Gene sequence:

>786_bases
ATGGACGATATTCTGATCACCGAGAAACGCGGGCACGTGACGGTGCTGACGCTGAACCGACCTGAGGCGATGAATTCGCT
CGATTATGAGCTTTATGACGCGCTGGAGAATGCGGTGCGCACCTCGGACGCGCGGGCGATTGTGATTACGGGGTCTGGCA
CGCGGGCGTTCTGCGCCGGGGACGATGTGAAGAAGATCCTCTCCAAGGGCGCGCCGGTGACGCCGGAGCGGGCGGCCAAG
GCGAAAGATACCGGCGGGCTGACGCCTGCGGCCGACGCCCTTCTGCATACGGATATTCCGGTGATTGCGGCGATCAATGG
CTTTGCGCTCGGCTGGGGCGCGGAGCTGGCGATCATGGCGGATATGCGGGTGATGTCTGACACCGCCAAGATCGGCGAGA
TTTTCGTGACGCGGGGGCTCTGCTGTGACGCGCCGGGGCTCGGGCGGCTGGCCCAGCTTGTGGGCCGCGAGAAGGCAAGC
GAGCTGCTGTTCACCGGGGACGTGATTGACGCGGCCGAGGCCAAGGCGATTGGCCTGGTGGGCCGGGTGGTGGCGCCGGG
GGACCTTATGCCGACGGCGCTGGCACTGGCGGAGAAGATCGCGGCGAACCCGCCCCAAGCGGTCAAATCGCTGAAAGCCG
GTTTGCGCCGGACGCTGGACCCGGATTGGCGCGATACGGGCAAATGGGCGATTACCGAAATCCGCCGCCTGATGCAGACC
GCCGACGCGAAGGAAAGCGCCGCGGCCTTCATCGAAAAGCGAACGCCGGTCTTCACGGGCAACTGA

Upstream 100 bases:

>100_bases
GGGGATATTTTCGGCATCCCCTGCCCTTACCTCGCGGCATTATTTTCAAGGCGGCGCCTTCATGAGAGCTTCCCCGGCAA
AAGATAGCCGGGAGGAAGAC

Downstream 100 bases:

>100_bases
TCGGGCGCTAGACAGGTGACCATGTTTCCAGATCGCCTTCGTAACCTCGCCATGCTGGTGCTCGATGATGCCGAGCGGGC
CCGCCTGAAGATCGTGACCG

Product: enoyl-CoA hydratase/isomerase family protein

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MDDILITEKRGHVTVLTLNRPEAMNSLDYELYDALENAVRTSDARAIVITGSGTRAFCAGDDVKKILSKGAPVTPERAAK
AKDTGGLTPAADALLHTDIPVIAAINGFALGWGAELAIMADMRVMSDTAKIGEIFVTRGLCCDAPGLGRLAQLVGREKAS
ELLFTGDVIDAAEAKAIGLVGRVVAPGDLMPTALALAEKIAANPPQAVKSLKAGLRRTLDPDWRDTGKWAITEIRRLMQT
ADAKESAAAFIEKRTPVFTGN

Sequences:

>Translated_261_residues
MDDILITEKRGHVTVLTLNRPEAMNSLDYELYDALENAVRTSDARAIVITGSGTRAFCAGDDVKKILSKGAPVTPERAAK
AKDTGGLTPAADALLHTDIPVIAAINGFALGWGAELAIMADMRVMSDTAKIGEIFVTRGLCCDAPGLGRLAQLVGREKAS
ELLFTGDVIDAAEAKAIGLVGRVVAPGDLMPTALALAEKIAANPPQAVKSLKAGLRRTLDPDWRDTGKWAITEIRRLMQT
ADAKESAAAFIEKRTPVFTGN
>Mature_261_residues
MDDILITEKRGHVTVLTLNRPEAMNSLDYELYDALENAVRTSDARAIVITGSGTRAFCAGDDVKKILSKGAPVTPERAAK
AKDTGGLTPAADALLHTDIPVIAAINGFALGWGAELAIMADMRVMSDTAKIGEIFVTRGLCCDAPGLGRLAQLVGREKAS
ELLFTGDVIDAAEAKAIGLVGRVVAPGDLMPTALALAEKIAANPPQAVKSLKAGLRRTLDPDWRDTGKWAITEIRRLMQT
ADAKESAAAFIEKRTPVFTGN

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=33.8403041825095, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=27.8884462151394, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=33.0882352941176, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136756, Length=260, Percent_Identity=21.1538461538462, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136753, Length=260, Percent_Identity=21.1538461538462, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221307494, Length=260, Percent_Identity=21.1538461538462, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI25453481, Length=263, Percent_Identity=21.2927756653992, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538573, Length=263, Percent_Identity=21.2927756653992, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4757968, Length=259, Percent_Identity=20.4633204633205, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=259, Percent_Identity=20.4633204633205, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=251, Percent_Identity=21.5139442231076, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538581, Length=251, Percent_Identity=21.5139442231076, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68989263, Length=177, Percent_Identity=32.2033898305085, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=35.8778625954198, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=31.7164179104478, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788597, Length=273, Percent_Identity=27.4725274725275, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=30.9433962264151, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=25.8823529411765, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788682, Length=195, Percent_Identity=32.3076923076923, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=36.0153256704981, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=32.9365079365079, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=33.1730769230769, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=27.7992277992278, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=26.8482490272374, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=24.8868778280543, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=258, Percent_Identity=23.2558139534884, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=226, Percent_Identity=26.9911504424779, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=35.7142857142857, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=35.7142857142857, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=35.7414448669202, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24650670, Length=204, Percent_Identity=34.3137254901961, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357171, Length=213, Percent_Identity=30.9859154929577, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=232, Percent_Identity=31.0344827586207, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=26.2548262548263, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27621; Mature: 27621

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDILITEKRGHVTVLTLNRPEAMNSLDYELYDALENAVRTSDARAIVITGSGTRAFCAG
CCCCEEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECC
DDVKKILSKGAPVTPERAAKAKDTGGLTPAADALLHTDIPVIAAINGFALGWGAELAIMA
HHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCEEECCCEEECCCCCEEEEH
DMRVMSDTAKIGEIFVTRGLCCDAPGLGRLAQLVGREKASELLFTGDVIDAAEAKAIGLV
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHH
GRVVAPGDLMPTALALAEKIAANPPQAVKSLKAGLRRTLDPDWRDTGKWAITEIRRLMQT
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ADAKESAAAFIEKRTPVFTGN
CCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDDILITEKRGHVTVLTLNRPEAMNSLDYELYDALENAVRTSDARAIVITGSGTRAFCAG
CCCCEEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECC
DDVKKILSKGAPVTPERAAKAKDTGGLTPAADALLHTDIPVIAAINGFALGWGAELAIMA
HHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCEEECCCEEECCCCCEEEEH
DMRVMSDTAKIGEIFVTRGLCCDAPGLGRLAQLVGREKASELLFTGDVIDAAEAKAIGLV
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHH
GRVVAPGDLMPTALALAEKIAANPPQAVKSLKAGLRRTLDPDWRDTGKWAITEIRRLMQT
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ADAKESAAAFIEKRTPVFTGN
CCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]