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The map label for this gene is oprM [H]

Identifier: 114797724

GI number: 114797724

Start: 3230592

End: 3232061

Strand: Direct

Name: oprM [H]

Synonym: HNE_3059

Alternate gene names: 114797724

Gene position: 3230592-3232061 (Clockwise)

Preceding gene: 114799479

Following gene: 114797172

Centisome position: 87.19

GC content: 65.58

Gene sequence:

>1470_bases
ATGAAAAATTCTCTCCTCCTGATGGGCACGGCCATGCTTGCGGCGTGTGCCACCTCTCCCAACGCCATTGAGCGTCTCGC
CACCGACAGGGAGGCTCTGTTTGCGGCTGCGCCCGAGGCGCCGGCCGAATGGGCGGCGCGCGGTGTTGCGGGTGTGGCTG
AAACGGGCAACTGGCTCGATCAGTTCAACGATCCGGTCATGAACGAACTTGTCGCTGAGGCCCTCGCCGCCAATCCGACA
CTGGAATCCCGCGCCGCCAGCCTGCGCGCCTCCGCTGCCCTGTCGCGCAGCGCGCGCGGCCAGCGCTGGCCCTCGCTATC
GGCGACCGCAAGCTTCGGTGGCACCTCCACCGGCGTAACTCTTCCCGCCGGCGGCGATGACCGCATTAATGGCGAAGTCT
ATGGCCTGGGCCTGGACGCAAGCTGGGAGTTCGACCTCTGGGGCCGTATCTCCAATGGCATTGCGCAGGCAGATGCTGAC
TATGCGGCAACCGCCGCCGACCTTGCCGCCACCGAACTTTCGATCGCTGCGCAGACCGCGTCGGCCTGGATCAGCCTCAA
TGAAGCGCTGACTCAGGAGCGGATTGCTGTTCTCACCTACGAGGCCCGTCAACGGGTGGTGGACCTGACCGAGCCGCGCG
TGCGCGCCGGCATCTATGGCCCGCTGGAATTGCGCACGGCGCGGAGCGTCCTCGCGCAAGCCGAAGCGGCCATCGCGGCG
CGGCGCCAGTTCTCGAATGAAGCCACCCGGCGTCTTGAGGTTCTGCTCGGCCGGTATCCCTCTGCGGAGCTGACTGCGCC
AGCGATCATTCCGGAATTGCCGCCCATGGAAGTTGTCGGCAATCCCGCATTAATGCTGTCGCGCCGGCCGGATATTGCCG
TGCTTGAAGCGCGCGTGGTCGGCGCCGGCCTGCAGGCTGAGGCCGCACGCCTTGCGCTGCTGCCGAGCCTTGGCCTGACC
GGATCGCTGGGCACGAATGAAACCGACATATCCGACGCGCTTGACCCGACCCTGATCGCGGCGCGCCTGATTGGAAATCT
GATACAGCCTCTCTTCAGTGGCGGCAGCCTCAATGCGCAACGCCTGGCCGCCATTGCTCAGGCTGAAGCTGCTGTGGCCA
ACTATGCCGGCGCCGCCCTGGATGCCTGGCGTGAGGTCGAGAACGCTCTGACGGCTGATATTCTTCTTGCTCAGCAGGAA
GACGCGCAGGGCCGGGCCCTCGAAGAAGCCCGCTTTGCCGAAGAGATTGCCGAGCGGCAGTATTCAAGCGGCACGGTCAA
CGTCTTTAACCTCATCGATGCGCAGACGCGCCGGCTGACCGCAGAAAGCCAGCTGGCAACGGCGCGGGCCGACCGTGCCC
GCAACCGGATTACCTATCATCTCGCCCTGGGCGGCGGCGTTCCCGTGATGGAACTCGCCGGCGAGGACGACCTGTTTGAC
ACTGACGCCGCCGAAAGCGGCAGGAGATAG

Upstream 100 bases:

>100_bases
ATCCGAGAGAAAAGTTATTGAAAATCGATAATTCCTCATTGACGCTCGGCAAGGACAGGCGTATCGCAACCCCAAGGCAG
CCCGGAGCGCCGGATACCGC

Downstream 100 bases:

>100_bases
ACGCCATGGGACGCCTGATCGCCATCATCGCACCAATTGCCATCCTTCTTGTTCTTGGCGTTGGAGGGGTCATTGCCGTC
ACGGCCATGAAGCCTGAGCC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKNSLLLMGTAMLAACATSPNAIERLATDREALFAAAPEAPAEWAARGVAGVAETGNWLDQFNDPVMNELVAEALAANPT
LESRAASLRASAALSRSARGQRWPSLSATASFGGTSTGVTLPAGGDDRINGEVYGLGLDASWEFDLWGRISNGIAQADAD
YAATAADLAATELSIAAQTASAWISLNEALTQERIAVLTYEARQRVVDLTEPRVRAGIYGPLELRTARSVLAQAEAAIAA
RRQFSNEATRRLEVLLGRYPSAELTAPAIIPELPPMEVVGNPALMLSRRPDIAVLEARVVGAGLQAEAARLALLPSLGLT
GSLGTNETDISDALDPTLIAARLIGNLIQPLFSGGSLNAQRLAAIAQAEAAVANYAGAALDAWREVENALTADILLAQQE
DAQGRALEEARFAEEIAERQYSSGTVNVFNLIDAQTRRLTAESQLATARADRARNRITYHLALGGGVPVMELAGEDDLFD
TDAAESGRR

Sequences:

>Translated_489_residues
MKNSLLLMGTAMLAACATSPNAIERLATDREALFAAAPEAPAEWAARGVAGVAETGNWLDQFNDPVMNELVAEALAANPT
LESRAASLRASAALSRSARGQRWPSLSATASFGGTSTGVTLPAGGDDRINGEVYGLGLDASWEFDLWGRISNGIAQADAD
YAATAADLAATELSIAAQTASAWISLNEALTQERIAVLTYEARQRVVDLTEPRVRAGIYGPLELRTARSVLAQAEAAIAA
RRQFSNEATRRLEVLLGRYPSAELTAPAIIPELPPMEVVGNPALMLSRRPDIAVLEARVVGAGLQAEAARLALLPSLGLT
GSLGTNETDISDALDPTLIAARLIGNLIQPLFSGGSLNAQRLAAIAQAEAAVANYAGAALDAWREVENALTADILLAQQE
DAQGRALEEARFAEEIAERQYSSGTVNVFNLIDAQTRRLTAESQLATARADRARNRITYHLALGGGVPVMELAGEDDLFD
TDAAESGRR
>Mature_489_residues
MKNSLLLMGTAMLAACATSPNAIERLATDREALFAAAPEAPAEWAARGVAGVAETGNWLDQFNDPVMNELVAEALAANPT
LESRAASLRASAALSRSARGQRWPSLSATASFGGTSTGVTLPAGGDDRINGEVYGLGLDASWEFDLWGRISNGIAQADAD
YAATAADLAATELSIAAQTASAWISLNEALTQERIAVLTYEARQRVVDLTEPRVRAGIYGPLELRTARSVLAQAEAAIAA
RRQFSNEATRRLEVLLGRYPSAELTAPAIIPELPPMEVVGNPALMLSRRPDIAVLEARVVGAGLQAEAARLALLPSLGLT
GSLGTNETDISDALDPTLIAARLIGNLIQPLFSGGSLNAQRLAAIAQAEAAVANYAGAALDAWREVENALTADILLAQQE
DAQGRALEEARFAEEIAERQYSSGTVNVFNLIDAQTRRLTAESQLATARADRARNRITYHLALGGGVPVMELAGEDDLFD
TDAAESGRR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=420, Percent_Identity=26.4285714285714, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI2367348, Length=439, Percent_Identity=26.879271070615, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51508; Mature: 51508

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSLLLMGTAMLAACATSPNAIERLATDREALFAAAPEAPAEWAARGVAGVAETGNWLD
CCCCEEEHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHCCCHHHHCCCHHH
QFNDPVMNELVAEALAANPTLESRAASLRASAALSRSARGQRWPSLSATASFGGTSTGVT
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
LPAGGDDRINGEVYGLGLDASWEFDLWGRISNGIAQADADYAATAADLAATELSIAAQTA
ECCCCCCCCCCEEEEEECCCCCCEEHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SAWISLNEALTQERIAVLTYEARQRVVDLTEPRVRAGIYGPLELRTARSVLAQAEAAIAA
HHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RRQFSNEATRRLEVLLGRYPSAELTAPAIIPELPPMEVVGNPALMLSRRPDIAVLEARVV
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHCCCCEEEEECCCCEEEEEHHHH
GAGLQAEAARLALLPSLGLTGSLGTNETDISDALDPTLIAARLIGNLIQPLFSGGSLNAQ
CCCCHHHHHHHHEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHH
RLAAIAQAEAAVANYAGAALDAWREVENALTADILLAQQEDAQGRALEEARFAEEIAERQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
YSSGTVNVFNLIDAQTRRLTAESQLATARADRARNRITYHLALGGGVPVMELAGEDDLFD
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEECCCCCCCC
TDAAESGRR
CCCCCCCCC
>Mature Secondary Structure
MKNSLLLMGTAMLAACATSPNAIERLATDREALFAAAPEAPAEWAARGVAGVAETGNWLD
CCCCEEEHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHCCCHHHHCCCHHH
QFNDPVMNELVAEALAANPTLESRAASLRASAALSRSARGQRWPSLSATASFGGTSTGVT
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
LPAGGDDRINGEVYGLGLDASWEFDLWGRISNGIAQADADYAATAADLAATELSIAAQTA
ECCCCCCCCCCEEEEEECCCCCCEEHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SAWISLNEALTQERIAVLTYEARQRVVDLTEPRVRAGIYGPLELRTARSVLAQAEAAIAA
HHHHHHHHHHHHHHHHEEEHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RRQFSNEATRRLEVLLGRYPSAELTAPAIIPELPPMEVVGNPALMLSRRPDIAVLEARVV
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHCCCCEEEEECCCCEEEEEHHHH
GAGLQAEAARLALLPSLGLTGSLGTNETDISDALDPTLIAARLIGNLIQPLFSGGSLNAQ
CCCCHHHHHHHHEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHH
RLAAIAQAEAAVANYAGAALDAWREVENALTADILLAQQEDAQGRALEEARFAEEIAERQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
YSSGTVNVFNLIDAQTRRLTAESQLATARADRARNRITYHLALGGGVPVMELAGEDDLFD
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEECCCCCCCC
TDAAESGRR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]