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The map label for this gene is rne [H]

Identifier: 114797667

GI number: 114797667

Start: 1773664

End: 1774734

Strand: Reverse

Name: rne [H]

Synonym: HNE_1732

Alternate gene names: 114797667

Gene position: 1774734-1773664 (Counterclockwise)

Preceding gene: 114798439

Following gene: 114799923

Centisome position: 47.9

GC content: 68.07

Gene sequence:

>1071_bases
ATGACAGTTGCCCGGTTCTTGCGAGAAGCCACGCTGGGCGAGACCCGTTGGGCGGCGCTCGATTTGCAGGACAGGCCGCT
CGCGCTCTATATTGAACGCCCCACAAAACCGGTGGTGCTCGGCGCCCGTCTTGAGGCCCGCATCGGCAAGACAGAGCCCG
GCGCAGGCGGGACTTTTATAGATATTCCAGGACAGTCGGGCGCCTTTCTCAGGACAAATCCGGGACAATCCGGGACAAAT
CAGGACACTTTGCTACCAAGCGAGGGCAGTCTCGTCACGGTGGACGTGGTGGCAGAAGGGCGCGCCGGAAAGCTGCCGCG
CGTCAAGCTCGCGCCCCCCGGCAGCCACCCTGCCCCCTCCGGCGCCAATGCCTGGCGGGCCGCACTGAGAGCCAGCCCCA
CAACACCGATTGAAGACGTGCCCGCCGGTGACAGCCGGATGGCCGCTGCATTTGAGGACGCGCTAAGCCCGGACGTCACC
TTGCCCGGCGGCGGGCGGCTGCGGATTGAACGGACGCGCGCGCTGACGGCGGCCGACATCGATTCGGCGGGCCGGGCGAT
GAAGGGCAGCGCCGCAGCGCGGGCGCTTTCGCTCAACAAGGAGGCGGCCGAGGCGCTGGCCCGGCAGATCCTCATTCGGG
GGCTGGGCGGGCTGTTCGTGCTCGACTGTGTGAGCCCGCTGACCAAGGAGACCTCCACAAAGCTGCGCGAGGCGTTCCTG
GCAGCGTGGGATGGCATGACGGCGCGCCCGGCGCGGGTGTTGGCGCCTTCGGCGCTGGGGCTGATGGAGCTGTCGACCGA
GTGGCGGATTACGCCGTTGAACGAGATGCTGCTGGATATGGCGGGCCTGCCGACGGCGGAGACGCTGGCACTTGCAGGGC
TGCGCCTGCTGGAAGCCGAGGCACGCCAGACCCGGATGGGGCGGCTGAAGCTGTCCCTGCCCCAAGCGGCGTTCGCCTGG
CTAACCGCCAGCGGCATGGACGCAGAGGCGCAGCTCGCCTCAAAATATGGCGCGCGGCTGACAATCGGGGTTCACGCCAG
GGCGGCCCCTGAGGTATCCCCCGAAGGATGA

Upstream 100 bases:

>100_bases
GGCAAGACGATGGCCGGGCGCATCAAGGCGGTGTTGAAAACCCCTGAGATTGCCGCCGATCCTGTAACGGCTGCGCTGCT
CAAGCAGATTTTCTGAGCGG

Downstream 100 bases:

>100_bases
GCAAACCTGTGAACCCAAGCCAGAGAACCCCGCTCTGCGCGCGCTGCCGCAAGCAGGCGGTCGCCGCCGAGTATCGCCCC
TTCTGTTCCAAGCGCTGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTVARFLREATLGETRWAALDLQDRPLALYIERPTKPVVLGARLEARIGKTEPGAGGTFIDIPGQSGAFLRTNPGQSGTN
QDTLLPSEGSLVTVDVVAEGRAGKLPRVKLAPPGSHPAPSGANAWRAALRASPTTPIEDVPAGDSRMAAAFEDALSPDVT
LPGGGRLRIERTRALTAADIDSAGRAMKGSAAARALSLNKEAAEALARQILIRGLGGLFVLDCVSPLTKETSTKLREAFL
AAWDGMTARPARVLAPSALGLMELSTEWRITPLNEMLLDMAGLPTAETLALAGLRLLEAEARQTRMGRLKLSLPQAAFAW
LTASGMDAEAQLASKYGARLTIGVHARAAPEVSPEG

Sequences:

>Translated_356_residues
MTVARFLREATLGETRWAALDLQDRPLALYIERPTKPVVLGARLEARIGKTEPGAGGTFIDIPGQSGAFLRTNPGQSGTN
QDTLLPSEGSLVTVDVVAEGRAGKLPRVKLAPPGSHPAPSGANAWRAALRASPTTPIEDVPAGDSRMAAAFEDALSPDVT
LPGGGRLRIERTRALTAADIDSAGRAMKGSAAARALSLNKEAAEALARQILIRGLGGLFVLDCVSPLTKETSTKLREAFL
AAWDGMTARPARVLAPSALGLMELSTEWRITPLNEMLLDMAGLPTAETLALAGLRLLEAEARQTRMGRLKLSLPQAAFAW
LTASGMDAEAQLASKYGARLTIGVHARAAPEVSPEG
>Mature_355_residues
TVARFLREATLGETRWAALDLQDRPLALYIERPTKPVVLGARLEARIGKTEPGAGGTFIDIPGQSGAFLRTNPGQSGTNQ
DTLLPSEGSLVTVDVVAEGRAGKLPRVKLAPPGSHPAPSGANAWRAALRASPTTPIEDVPAGDSRMAAAFEDALSPDVTL
PGGGRLRIERTRALTAADIDSAGRAMKGSAAARALSLNKEAAEALARQILIRGLGGLFVLDCVSPLTKETSTKLREAFLA
AWDGMTARPARVLAPSALGLMELSTEWRITPLNEMLLDMAGLPTAETLALAGLRLLEAEARQTRMGRLKLSLPQAAFAWL
TASGMDAEAQLASKYGARLTIGVHARAAPEVSPEG

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=188, Percent_Identity=31.3829787234043, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 37388; Mature: 37257

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVARFLREATLGETRWAALDLQDRPLALYIERPTKPVVLGARLEARIGKTEPGAGGTFI
CCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCEEECCEEHEECCCCCCCCCCCEE
DIPGQSGAFLRTNPGQSGTNQDTLLPSEGSLVTVDVVAEGRAGKLPRVKLAPPGSHPAPS
ECCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCC
GANAWRAALRASPTTPIEDVPAGDSRMAAAFEDALSPDVTLPGGGRLRIERTRALTAADI
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEHHHEEEECCC
DSAGRAMKGSAAARALSLNKEAAEALARQILIRGLGGLFVLDCVSPLTKETSTKLREAFL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AAWDGMTARPARVLAPSALGLMELSTEWRITPLNEMLLDMAGLPTAETLALAGLRLLEAE
HHHCCCCCCCHHHCCCCHHHHEEECCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ARQTRMGRLKLSLPQAAFAWLTASGMDAEAQLASKYGARLTIGVHARAAPEVSPEG
HHHHHHCEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TVARFLREATLGETRWAALDLQDRPLALYIERPTKPVVLGARLEARIGKTEPGAGGTFI
CHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCEEECCEEHEECCCCCCCCCCCEE
DIPGQSGAFLRTNPGQSGTNQDTLLPSEGSLVTVDVVAEGRAGKLPRVKLAPPGSHPAPS
ECCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCC
GANAWRAALRASPTTPIEDVPAGDSRMAAAFEDALSPDVTLPGGGRLRIERTRALTAADI
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEHHHEEEECCC
DSAGRAMKGSAAARALSLNKEAAEALARQILIRGLGGLFVLDCVSPLTKETSTKLREAFL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AAWDGMTARPARVLAPSALGLMELSTEWRITPLNEMLLDMAGLPTAETLALAGLRLLEAE
HHHCCCCCCCHHHCCCCHHHHEEECCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ARQTRMGRLKLSLPQAAFAWLTASGMDAEAQLASKYGARLTIGVHARAAPEVSPEG
HHHHHHCEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]