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The map label for this gene is gstA [H]

Identifier: 114797640

GI number: 114797640

Start: 2719034

End: 2719729

Strand: Reverse

Name: gstA [H]

Synonym: HNE_2566

Alternate gene names: 114797640

Gene position: 2719729-2719034 (Counterclockwise)

Preceding gene: 114798001

Following gene: 114799610

Centisome position: 73.41

GC content: 63.07

Gene sequence:

>696_bases
GTGGGGCGCTGCGGGCCGGTTGACGGACGCAAGCGGGCGTTTCAAGCTGTGGGAAATAACATCAAAAAGCGGGGAAACGC
CTTGAAACTTTACAATACGCCGATGCCGGCGCCCAATCCGCGCCGGGTGCGCATGTTTCTGGCTGAGAAGGGCGTTTCGG
TCCCTCTGGTGGACGTGGCGCTGGTGAAGGGGGAGCATAAGAGCGCCGATCACGTGGCGCGTAACTCGCTGGGGCAGACG
CCGGTGCTGGAGCTGGATGACGGGACGTGCATCAGCGAGACAGTGGCGATCTGCCGGTATCTGGAGAGCGTTTATCCTGA
GCCGAACCTCTTTGGAATGGATGGGCTGGAAGCGGCGCTGATCGACATGTGGGTGCGGCGGGTGGAATTTCAGCTGATGA
GCCCGGCGGGCATGTTCTGGCGGCATGCTCATCCGTTCACGGCGAAGGTGGTGGTTCCTCAGTATCAGGAGTTTGGCGCG
TCAAACCGCGGGCATTATGAGCGCGCGCTGATGTGGCTGGATGGGGAACTGGCCGGGCGGGATTATGTGGCCGGCGAGCG
GTTTACGATTGCCGATATTTGCGCGCTGTCGACGGTGGATTTTGCCGGCTGGATTGGGCTGCCGGTGCCGGACGGGGCGG
GGCATGTGAAGGCGTGGCATGAGCGGGTGAGCGCGCGGGAGAGTGCGGGGGCTTGA

Upstream 100 bases:

>100_bases
TCTTTGTGAGGTGCGTCTCACCCTACCAACGCGTCTTAGAACGGATCGTTCCATTTTAAATCCTGCAATTGGATTGATCC
GTAGGGAGAGGTCTTTTTGC

Downstream 100 bases:

>100_bases
ACACTCCGCTGCGCCTCAGTGGTTTGCGGACCTTGGCGCGAGCGCCGACGCCCAGGCGAGTTCAAAAGCAGAGGTGAGGA
GATTTGCATCGCCTCCGCCG

Product: glutathione S-transferase

Products: HX; R-S-Glutathione; S-(2,4-dinitrophenyl)glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MGRCGPVDGRKRAFQAVGNNIKKRGNALKLYNTPMPAPNPRRVRMFLAEKGVSVPLVDVALVKGEHKSADHVARNSLGQT
PVLELDDGTCISETVAICRYLESVYPEPNLFGMDGLEAALIDMWVRRVEFQLMSPAGMFWRHAHPFTAKVVVPQYQEFGA
SNRGHYERALMWLDGELAGRDYVAGERFTIADICALSTVDFAGWIGLPVPDGAGHVKAWHERVSARESAGA

Sequences:

>Translated_231_residues
MGRCGPVDGRKRAFQAVGNNIKKRGNALKLYNTPMPAPNPRRVRMFLAEKGVSVPLVDVALVKGEHKSADHVARNSLGQT
PVLELDDGTCISETVAICRYLESVYPEPNLFGMDGLEAALIDMWVRRVEFQLMSPAGMFWRHAHPFTAKVVVPQYQEFGA
SNRGHYERALMWLDGELAGRDYVAGERFTIADICALSTVDFAGWIGLPVPDGAGHVKAWHERVSARESAGA
>Mature_230_residues
GRCGPVDGRKRAFQAVGNNIKKRGNALKLYNTPMPAPNPRRVRMFLAEKGVSVPLVDVALVKGEHKSADHVARNSLGQTP
VLELDDGTCISETVAICRYLESVYPEPNLFGMDGLEAALIDMWVRRVEFQLMSPAGMFWRHAHPFTAKVVVPQYQEFGAS
NRGHYERALMWLDGELAGRDYVAGERFTIADICALSTVDFAGWIGLPVPDGAGHVKAWHERVSARESAGA

Specific function: Conjugation Of Reduced Glutathione To A Wide Number Of Exogenous And Endogenous Hydrophobic Electrophiles. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4504187, Length=201, Percent_Identity=30.3482587064677, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI124249394, Length=201, Percent_Identity=30.3482587064677, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322968, Length=215, Percent_Identity=37.2093023255814, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI20130123, Length=203, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18 [C]

Molecular weight: Translated: 25433; Mature: 25302

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRCGPVDGRKRAFQAVGNNIKKRGNALKLYNTPMPAPNPRRVRMFLAEKGVSVPLVDVA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHEEEEEHHCCCCCCEEHEE
LVKGEHKSADHVARNSLGQTPVLELDDGTCISETVAICRYLESVYPEPNLFGMDGLEAAL
EECCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IDMWVRRVEFQLMSPAGMFWRHAHPFTAKVVVPQYQEFGASNRGHYERALMWLDGELAGR
HHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCHHHCCCCCCCCHHHEEEEECCCCCCC
DYVAGERFTIADICALSTVDFAGWIGLPVPDGAGHVKAWHERVSARESAGA
CCCCCCCCCHHHHHHHHHHHHHCEECCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GRCGPVDGRKRAFQAVGNNIKKRGNALKLYNTPMPAPNPRRVRMFLAEKGVSVPLVDVA
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHEEEEEHHCCCCCCEEHEE
LVKGEHKSADHVARNSLGQTPVLELDDGTCISETVAICRYLESVYPEPNLFGMDGLEAAL
EECCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IDMWVRRVEFQLMSPAGMFWRHAHPFTAKVVVPQYQEFGASNRGHYERALMWLDGELAGR
HHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCHHHCCCCCCCCHHHEEEEECCCCCCC
DYVAGERFTIADICALSTVDFAGWIGLPVPDGAGHVKAWHERVSARESAGA
CCCCCCCCCHHHHHHHHHHHHHCEECCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; Glutathione; 1-chloro-2,4-dinitrobenzene; reduced glutathione [C]

Specific reaction: RX + Glutathione = HX + R-S-Glutathione 1-chloro-2,4-dinitrobenzene + reduced glutathione = S-(2,4-dinitrophenyl)glutathione [C]

General reaction: Aryl group transfer [C]

Inhibitor: Cd2+; CibacronBlue; Co2+; Cu2+; Hematin; Hg2+; Ni2+; Sulfophthaleins; Tributyl tinacetate; Triethyl tinbromide; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA