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The map label for this gene is vlacs [H]

Identifier: 114798001

GI number: 114798001

Start: 2723343

End: 2725133

Strand: Reverse

Name: vlacs [H]

Synonym: HNE_2570

Alternate gene names: 114798001

Gene position: 2725133-2723343 (Counterclockwise)

Preceding gene: 114798734

Following gene: 114797640

Centisome position: 73.55

GC content: 60.86

Gene sequence:

>1791_bases
ATGAACCTTTTTGCCAAACTTGGCCGCGAATACCGGCTGTTTCAGGATATTCTGCTCGTCAAGAAATGGACGGGGGACAT
TTCCCCGGACTCTGACCATCTGGTGGCGGATGATTACGAACAGGCGGTGGACAAGTTTTCCGCCAATGTCGCGTTCCGCT
TCGAGGGCAAATCGACCACCTATTCGGAGTTTGACGAAACGGCGAGCCGGTTTGCCAACTGGGCGCTGGCGCAGGGCCTG
AAGGCGGGCGACTGTATCGCGCTGTTCATGGAAAACCGCCCGGAATATGTGGCGGCCTGGGCGGGCTTTGCGAAGATCGG
TGTCGTCACGGCGCTGATCAATCACAATCTTGAAAACGACGCGCTGGCCCATTGCGTGAATATTTCCGAGGCCAAGCTGA
TCGTGACGGGGGCGGACCAGGACGCGGCGATCACCGGGGCGGCGGGACTCTTCAAGGACGCGCCGAAGGTGTGGTCTCTG
GGCGGGGCTGTGGGTGAAGACCTTGGCGGGGCGCTGGCGGGGGCGTCGTCTGCGCGGCCGGACCGGTCGCACCGGGCCGG
GCTGCTGGGTAAGGATCTCTGCCTTTATGTTTATACCTCCGGCACGACCGGCTTGCCCAAGGCCGCGCGCCTGACGCAGG
CCCGCACGCAGGGCATGATGAAAAGCTTCATTGCGCCGTGCCGCATCACGCCGAAGGACCGGGTTTATATCACCCTGCCG
CTGTATCATGGCACGGGCGGACTTTGCGGGGTTGGCCAGGCGCTGATGACGGGCGCAACGATTATCCTGAGACGGAAGTT
TTCTGCGAGCGCCTTCTGGGACGACGCGACGGACGAGGGCGCGACCGCCATCGTTTATATCGGGGAACTTTGCCGCTACC
TCGTGAACTCGCCGCCCCACCCGAAGGAGCGCGCGCACCATATCCGCACTGGCTTTGGCAATGGCCTGCGCCCGGAAGTG
TGGGAAGAGTTTCTCGAGCGGTTCAACATTCCCCATCTCGCAGAGTTTTACGGCTCGACGGAAGGCAATGTCAGCTTCAT
CAATTTTGACGGCAAGCCCGGCGCTATCGGGCGTATTCCGGGCTGGCTGAAAAGCCAGTTTGCGCATGTCGGCTTTGTGA
AATTCGACATCGAGACGGAGCAGCCTGTGCGAGGGCCGGATGGTTTCTGTATTCCGGCGGCGGATGATGAGCCGGGCGAA
GCGATTGGCAAGATCGGCGACGATGTGCGCCAGCGCTTTGAAGGCTATAACGACCAGAAGGCGACCGAGAAGAAGCTGCT
GCGCGATGTGTTCGAGAAAGGCGATCTCTGGTTCCGCACCGGGGACCTGCTGAAGAAGGACAAGGCCGGCTATATCTATT
TCGTGGACCGGATCGGCGATACCTATCGCTGGAAGGGCGAGAATGTCTCGACCAATGAAGTGGGCGAGGCCTTGTCGAAG
ATTGACGGTATCGCCACGGCCAACGTCTATGGCGTGCCTGTTCCGGGAACGGACGGCAAGGCCGGTATGGCGGCGGTGAC
GCTCGACGGGGCAGTGGATATGCCGGGCGTTTACAAGCGCCTTGCCGCGCTGCTGCCGTCTTATTCGGTGCCGATCTTCA
TTCGTGTGCAGCCGGAAGCGGAAACGACCGGCACGTTCAAGTATCGCAAAGTGGAGCTGGTGGCCGAGGGATTTGATCCG
TCCAAGGTCGAAGGCGACGCTGTGTGGATGTATGATCCCGCTGAGGGCGGCTATGCGCCGGTGACGCCAGCGCGGTATGA
GAAGCTGCTGGCGGGTGGGTTTAAGTTTTAA

Upstream 100 bases:

>100_bases
GTTAGCGAAATCTTTGACAATGTCTGCGAAACTCCCATCTGTTAATTTCTGATAAAACCCGAAAATCTTATCCGACCCTC
TGCGTTGGGAGGCGCCGCAC

Downstream 100 bases:

>100_bases
GGCTTAGACGAAATCCCTATATTCTTTTGACGGGTTGCCGGGTTGAGAGCGCCCCCACCCTAGCCCTCCCCCATGTCTGG
GGGAGGGGATCAGGTTGTGC

Product: long-chain-acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MNLFAKLGREYRLFQDILLVKKWTGDISPDSDHLVADDYEQAVDKFSANVAFRFEGKSTTYSEFDETASRFANWALAQGL
KAGDCIALFMENRPEYVAAWAGFAKIGVVTALINHNLENDALAHCVNISEAKLIVTGADQDAAITGAAGLFKDAPKVWSL
GGAVGEDLGGALAGASSARPDRSHRAGLLGKDLCLYVYTSGTTGLPKAARLTQARTQGMMKSFIAPCRITPKDRVYITLP
LYHGTGGLCGVGQALMTGATIILRRKFSASAFWDDATDEGATAIVYIGELCRYLVNSPPHPKERAHHIRTGFGNGLRPEV
WEEFLERFNIPHLAEFYGSTEGNVSFINFDGKPGAIGRIPGWLKSQFAHVGFVKFDIETEQPVRGPDGFCIPAADDEPGE
AIGKIGDDVRQRFEGYNDQKATEKKLLRDVFEKGDLWFRTGDLLKKDKAGYIYFVDRIGDTYRWKGENVSTNEVGEALSK
IDGIATANVYGVPVPGTDGKAGMAAVTLDGAVDMPGVYKRLAALLPSYSVPIFIRVQPEAETTGTFKYRKVELVAEGFDP
SKVEGDAVWMYDPAEGGYAPVTPARYEKLLAGGFKF

Sequences:

>Translated_596_residues
MNLFAKLGREYRLFQDILLVKKWTGDISPDSDHLVADDYEQAVDKFSANVAFRFEGKSTTYSEFDETASRFANWALAQGL
KAGDCIALFMENRPEYVAAWAGFAKIGVVTALINHNLENDALAHCVNISEAKLIVTGADQDAAITGAAGLFKDAPKVWSL
GGAVGEDLGGALAGASSARPDRSHRAGLLGKDLCLYVYTSGTTGLPKAARLTQARTQGMMKSFIAPCRITPKDRVYITLP
LYHGTGGLCGVGQALMTGATIILRRKFSASAFWDDATDEGATAIVYIGELCRYLVNSPPHPKERAHHIRTGFGNGLRPEV
WEEFLERFNIPHLAEFYGSTEGNVSFINFDGKPGAIGRIPGWLKSQFAHVGFVKFDIETEQPVRGPDGFCIPAADDEPGE
AIGKIGDDVRQRFEGYNDQKATEKKLLRDVFEKGDLWFRTGDLLKKDKAGYIYFVDRIGDTYRWKGENVSTNEVGEALSK
IDGIATANVYGVPVPGTDGKAGMAAVTLDGAVDMPGVYKRLAALLPSYSVPIFIRVQPEAETTGTFKYRKVELVAEGFDP
SKVEGDAVWMYDPAEGGYAPVTPARYEKLLAGGFKF
>Mature_596_residues
MNLFAKLGREYRLFQDILLVKKWTGDISPDSDHLVADDYEQAVDKFSANVAFRFEGKSTTYSEFDETASRFANWALAQGL
KAGDCIALFMENRPEYVAAWAGFAKIGVVTALINHNLENDALAHCVNISEAKLIVTGADQDAAITGAAGLFKDAPKVWSL
GGAVGEDLGGALAGASSARPDRSHRAGLLGKDLCLYVYTSGTTGLPKAARLTQARTQGMMKSFIAPCRITPKDRVYITLP
LYHGTGGLCGVGQALMTGATIILRRKFSASAFWDDATDEGATAIVYIGELCRYLVNSPPHPKERAHHIRTGFGNGLRPEV
WEEFLERFNIPHLAEFYGSTEGNVSFINFDGKPGAIGRIPGWLKSQFAHVGFVKFDIETEQPVRGPDGFCIPAADDEPGE
AIGKIGDDVRQRFEGYNDQKATEKKLLRDVFEKGDLWFRTGDLLKKDKAGYIYFVDRIGDTYRWKGENVSTNEVGEALSK
IDGIATANVYGVPVPGTDGKAGMAAVTLDGAVDMPGVYKRLAALLPSYSVPIFIRVQPEAETTGTFKYRKVELVAEGFDP
SKVEGDAVWMYDPAEGGYAPVTPARYEKLLAGGFKF

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807357, Length=565, Percent_Identity=44.070796460177, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI38524616, Length=552, Percent_Identity=42.0289855072464, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI62865631, Length=552, Percent_Identity=39.1304347826087, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI13325055, Length=552, Percent_Identity=39.1304347826087, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI227499619, Length=554, Percent_Identity=40.2527075812274, Blast_Score=408, Evalue=1e-113,
Organism=Homo sapiens, GI13236579, Length=531, Percent_Identity=40.30131826742, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI13325057, Length=556, Percent_Identity=39.568345323741, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI227499621, Length=551, Percent_Identity=37.0235934664247, Blast_Score=349, Evalue=4e-96,
Organism=Escherichia coli, GI221142682, Length=442, Percent_Identity=28.5067873303167, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI145693145, Length=486, Percent_Identity=26.3374485596708, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17551278, Length=559, Percent_Identity=39.5348837209302, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI115534440, Length=558, Percent_Identity=40.3225806451613, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563687, Length=491, Percent_Identity=23.8289205702648, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319515, Length=513, Percent_Identity=33.9181286549708, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319699, Length=503, Percent_Identity=23.6580516898608, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364798, Length=582, Percent_Identity=39.5189003436426, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45550496, Length=588, Percent_Identity=39.9659863945578, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45552801, Length=588, Percent_Identity=39.9659863945578, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24583465, Length=582, Percent_Identity=39.5189003436426, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24583463, Length=582, Percent_Identity=39.5189003436426, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI28573631, Length=614, Percent_Identity=36.6449511400651, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI20130353, Length=608, Percent_Identity=36.8421052631579, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI21355181, Length=451, Percent_Identity=24.8337028824834, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24581924, Length=522, Percent_Identity=24.3295019157088, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 64916; Mature: 64916

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFAKLGREYRLFQDILLVKKWTGDISPDSDHLVADDYEQAVDKFSANVAFRFEGKSTT
CCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCEEEEECCCCCC
YSEFDETASRFANWALAQGLKAGDCIALFMENRPEYVAAWAGFAKIGVVTALINHNLEND
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCHH
ALAHCVNISEAKLIVTGADQDAAITGAAGLFKDAPKVWSLGGAVGEDLGGALAGASSARP
HHHHHCCCCCCEEEEECCCCCCEEECCHHHHHCCCHHHHCCCHHHHHHCHHHCCCCCCCC
DRSHRAGLLGKDLCLYVYTSGTTGLPKAARLTQARTQGMMKSFIAPCRITPKDRVYITLP
CCHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
LYHGTGGLCGVGQALMTGATIILRRKFSASAFWDDATDEGATAIVYIGELCRYLVNSPPH
EEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCC
PKERAHHIRTGFGNGLRPEVWEEFLERFNIPHLAEFYGSTEGNVSFINFDGKPGAIGRIP
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCC
GWLKSQFAHVGFVKFDIETEQPVRGPDGFCIPAADDEPGEAIGKIGDDVRQRFEGYNDQK
HHHHHHHHHCCEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCH
ATEKKLLRDVFEKGDLWFRTGDLLKKDKAGYIYFVDRIGDTYRWKGENVSTNEVGEALSK
HHHHHHHHHHHHCCCEEEECCCHHHCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHH
IDGIATANVYGVPVPGTDGKAGMAAVTLDGAVDMPGVYKRLAALLPSYSVPIFIRVQPEA
HCCCEECCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCC
ETTGTFKYRKVELVAEGFDPSKVEGDAVWMYDPAEGGYAPVTPARYEKLLAGGFKF
CCCCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLFAKLGREYRLFQDILLVKKWTGDISPDSDHLVADDYEQAVDKFSANVAFRFEGKSTT
CCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCEEEEECCCCCC
YSEFDETASRFANWALAQGLKAGDCIALFMENRPEYVAAWAGFAKIGVVTALINHNLEND
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCHH
ALAHCVNISEAKLIVTGADQDAAITGAAGLFKDAPKVWSLGGAVGEDLGGALAGASSARP
HHHHHCCCCCCEEEEECCCCCCEEECCHHHHHCCCHHHHCCCHHHHHHCHHHCCCCCCCC
DRSHRAGLLGKDLCLYVYTSGTTGLPKAARLTQARTQGMMKSFIAPCRITPKDRVYITLP
CCHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
LYHGTGGLCGVGQALMTGATIILRRKFSASAFWDDATDEGATAIVYIGELCRYLVNSPPH
EEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCC
PKERAHHIRTGFGNGLRPEVWEEFLERFNIPHLAEFYGSTEGNVSFINFDGKPGAIGRIP
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCC
GWLKSQFAHVGFVKFDIETEQPVRGPDGFCIPAADDEPGEAIGKIGDDVRQRFEGYNDQK
HHHHHHHHHCCEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCH
ATEKKLLRDVFEKGDLWFRTGDLLKKDKAGYIYFVDRIGDTYRWKGENVSTNEVGEALSK
HHHHHHHHHHHHCCCEEEECCCHHHCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHH
IDGIATANVYGVPVPGTDGKAGMAAVTLDGAVDMPGVYKRLAALLPSYSVPIFIRVQPEA
HCCCEECCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCC
ETTGTFKYRKVELVAEGFDPSKVEGDAVWMYDPAEGGYAPVTPARYEKLLAGGFKF
CCCCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA