Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 114797585

GI number: 114797585

Start: 2730114

End: 2731781

Strand: Direct

Name: nadE [H]

Synonym: HNE_2577

Alternate gene names: 114797585

Gene position: 2730114-2731781 (Clockwise)

Preceding gene: 114800543

Following gene: 114799057

Centisome position: 73.69

GC content: 64.27

Gene sequence:

>1668_bases
ATGCTCATCATGAGCAATAATCTTCGCATTCTGGCGGCCCAGCTGAACCCCGTTGTGGGCGACATCAAAGGCAATCTGGC
GCTTGCGCGGGAGGCCTATGGCGAGGCCCGCGCCAAGGGTGCCCATCTTCTCGTGCTGAGCGAGCATTTCCTGCTGGGCT
ACCCGGCCGAGGACCTCGTGCTGAAACCGTCGGCGGTCGAGCAGTCGATGGACGCCGTGCGCGCGCTGGCACAGGAAACA
AAGGATGGCCCGGCCATTCTCATCGGTACACCCTGGTCTGAAGAGGGCCGGCTCTACAACTCCGCCTGCTTCCTCGGCGG
CGGCCTGATCAAGGCGCGCTACGACAAACGCGAACTGCCCAATTACGGCGTGTTCGACGAGAAGCGCCACTACACGCCCG
GCGAAGGCCCGCCTCTGGTGGTGGAGCTGCACGGCGTGCGCGTCGGCGTGGCAATCTGTGAGGACATCTGGCTGGAGCGC
GTGCCCCGCGCCACCCATGCAGCCGGCGCCGAGCTGCTGATCGTGCCCAACGCCTCGCCCTGGCGCCGTGCCATCCAGGC
CGAACGCGCCAACGCCTTCGACCGCTGGGCCGACCTGAAACTGCCCTACCTGTTCGTCAACCAGATGGGCGGGCAGGACG
AGCTCGTTTTCGATGGCGCCTCCTTTGCCACCAACGGAACGCATGGCGAGCGCTGCGGCCTCGTCGGCCAGTTCGAGACC
GGCACAGCGCTCGTCGAATTTGATCCGTCCACGAAGCAATTCTCCGGTCTCGGCCAGAGCTGCGCGGCAGACCTTGAAGG
CTGGCCCGCCGAATATCGCGCCGCCATGCAGGGGCTGGGCGATTATGTGCGCAAGAACCGCTTCCCCGGCGTCGTGCTTG
GCATGTCGGGCGGCATCGACAGCGCCATCACCGCCGCCATCGCCGCCGATGCTCTGGGCCCGGACAAGGTCTGGTGCGTG
ATGATGCCATCGAAATATACCAGCAATGACAGCCTCGAAGACGCCAAAGCCTGCGCGCAGGCGCTCGGCTGCCGCTATGA
CACGATCAATATCCATCCGGGCGTCGAGGCGCTGGACACGATGCTGGGCGACGTCTTTGCGGGCAAGAAGGCAGACATCA
CCGAAGAGAACATCCAGTCCCGCCTGCGCGCGGTCACCCTGATGGCGCTGTCCAACAAGTTCGGCCAGATGGTCGTCACC
ACCGGCAACAAGAGCGAGATGGCCGTTGGCTACGCCACGCTCTATGGCGACATGTGCGGAGGCTACAACGCGCTGAAGGA
TTTCTACAAGACCGAAGTGTTCGAGCTGTCGAAATGGCGCAATGCGGTGCACCCCAAAGACGCGCTCGCGCCCGCCGGTG
AAGTGATCCCGGAACGGATCATCACCAAACCGCCCTCGGCCGAACTGCGCGAAGACCAGAAGGACGAGGATTCCCTGCCG
CCCTATGAAGTGCTGGACGACATCCTGCGCGGGCTGGTGGACCTGGAAGAAGACGTGGACGACATTCTCGCGCGCGGCCA
TGACGCGGCCACCGTGCGGCGGATTGAACATCTCGTCTACATCGCCGAATACAAGCGCCGCCAAGCTCCGCCGGGCGTGA
AAGTCGGCGGCAAGAATTTCGGCCGCGACCGGCGCTATCCGATCACCAACCGCTTCCGGGACGATTGA

Upstream 100 bases:

>100_bases
CCACCCGAACACCCCGCAAACCCGGATGGCCCCGCCAGTCCGGCTAAAGGCGTATGGAGAGGGCCCATCCCCTGATGACA
TCGCCCGCGCATCATCCTAA

Downstream 100 bases:

>100_bases
GGCGTTATTCTACCGTTGGCGGCTCGATGGTCGACGCGGTCGGGTCCAGCCCGAAAGAATCGATCGCCATCATTTCGCGC
ACCGTCTCGACCTCTTCGGG

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLIMSNNLRILAAQLNPVVGDIKGNLALAREAYGEARAKGAHLLVLSEHFLLGYPAEDLVLKPSAVEQSMDAVRALAQET
KDGPAILIGTPWSEEGRLYNSACFLGGGLIKARYDKRELPNYGVFDEKRHYTPGEGPPLVVELHGVRVGVAICEDIWLER
VPRATHAAGAELLIVPNASPWRRAIQAERANAFDRWADLKLPYLFVNQMGGQDELVFDGASFATNGTHGERCGLVGQFET
GTALVEFDPSTKQFSGLGQSCAADLEGWPAEYRAAMQGLGDYVRKNRFPGVVLGMSGGIDSAITAAIAADALGPDKVWCV
MMPSKYTSNDSLEDAKACAQALGCRYDTINIHPGVEALDTMLGDVFAGKKADITEENIQSRLRAVTLMALSNKFGQMVVT
TGNKSEMAVGYATLYGDMCGGYNALKDFYKTEVFELSKWRNAVHPKDALAPAGEVIPERIITKPPSAELREDQKDEDSLP
PYEVLDDILRGLVDLEEDVDDILARGHDAATVRRIEHLVYIAEYKRRQAPPGVKVGGKNFGRDRRYPITNRFRDD

Sequences:

>Translated_555_residues
MLIMSNNLRILAAQLNPVVGDIKGNLALAREAYGEARAKGAHLLVLSEHFLLGYPAEDLVLKPSAVEQSMDAVRALAQET
KDGPAILIGTPWSEEGRLYNSACFLGGGLIKARYDKRELPNYGVFDEKRHYTPGEGPPLVVELHGVRVGVAICEDIWLER
VPRATHAAGAELLIVPNASPWRRAIQAERANAFDRWADLKLPYLFVNQMGGQDELVFDGASFATNGTHGERCGLVGQFET
GTALVEFDPSTKQFSGLGQSCAADLEGWPAEYRAAMQGLGDYVRKNRFPGVVLGMSGGIDSAITAAIAADALGPDKVWCV
MMPSKYTSNDSLEDAKACAQALGCRYDTINIHPGVEALDTMLGDVFAGKKADITEENIQSRLRAVTLMALSNKFGQMVVT
TGNKSEMAVGYATLYGDMCGGYNALKDFYKTEVFELSKWRNAVHPKDALAPAGEVIPERIITKPPSAELREDQKDEDSLP
PYEVLDDILRGLVDLEEDVDDILARGHDAATVRRIEHLVYIAEYKRRQAPPGVKVGGKNFGRDRRYPITNRFRDD
>Mature_555_residues
MLIMSNNLRILAAQLNPVVGDIKGNLALAREAYGEARAKGAHLLVLSEHFLLGYPAEDLVLKPSAVEQSMDAVRALAQET
KDGPAILIGTPWSEEGRLYNSACFLGGGLIKARYDKRELPNYGVFDEKRHYTPGEGPPLVVELHGVRVGVAICEDIWLER
VPRATHAAGAELLIVPNASPWRRAIQAERANAFDRWADLKLPYLFVNQMGGQDELVFDGASFATNGTHGERCGLVGQFET
GTALVEFDPSTKQFSGLGQSCAADLEGWPAEYRAAMQGLGDYVRKNRFPGVVLGMSGGIDSAITAAIAADALGPDKVWCV
MMPSKYTSNDSLEDAKACAQALGCRYDTINIHPGVEALDTMLGDVFAGKKADITEENIQSRLRAVTLMALSNKFGQMVVT
TGNKSEMAVGYATLYGDMCGGYNALKDFYKTEVFELSKWRNAVHPKDALAPAGEVIPERIITKPPSAELREDQKDEDSLP
PYEVLDDILRGLVDLEEDVDDILARGHDAATVRRIEHLVYIAEYKRRQAPPGVKVGGKNFGRDRRYPITNRFRDD

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60717; Mature: 60717

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIMSNNLRILAAQLNPVVGDIKGNLALAREAYGEARAKGAHLLVLSEHFLLGYPAEDLV
CEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCCCHHCE
LKPSAVEQSMDAVRALAQETKDGPAILIGTPWSEEGRLYNSACFLGGGLIKARYDKRELP
ECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCEECCCEEEEECCHHCCC
NYGVFDEKRHYTPGEGPPLVVELHGVRVGVAICEDIWLERVPRATHAAGAELLIVPNASP
CCCCCCCCCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCH
WRRAIQAERANAFDRWADLKLPYLFVNQMGGQDELVFDGASFATNGTHGERCGLVGQFET
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCEEEEECC
GTALVEFDPSTKQFSGLGQSCAADLEGWPAEYRAAMQGLGDYVRKNRFPGVVLGMSGGID
CCEEEEECCCHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
SAITAAIAADALGPDKVWCVMMPSKYTSNDSLEDAKACAQALGCRYDTINIHPGVEALDT
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHH
MLGDVFAGKKADITEENIQSRLRAVTLMALSNKFGQMVVTTGNKSEMAVGYATLYGDMCG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHCC
GYNALKDFYKTEVFELSKWRNAVHPKDALAPAGEVIPERIITKPPSAELREDQKDEDSLP
CHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCC
PYEVLDDILRGLVDLEEDVDDILARGHDAATVRRIEHLVYIAEYKRRQAPPGVKVGGKNF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
GRDRRYPITNRFRDD
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MLIMSNNLRILAAQLNPVVGDIKGNLALAREAYGEARAKGAHLLVLSEHFLLGYPAEDLV
CEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCCCHHCE
LKPSAVEQSMDAVRALAQETKDGPAILIGTPWSEEGRLYNSACFLGGGLIKARYDKRELP
ECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCEECCCEEEEECCHHCCC
NYGVFDEKRHYTPGEGPPLVVELHGVRVGVAICEDIWLERVPRATHAAGAELLIVPNASP
CCCCCCCCCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCH
WRRAIQAERANAFDRWADLKLPYLFVNQMGGQDELVFDGASFATNGTHGERCGLVGQFET
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCEEEEECC
GTALVEFDPSTKQFSGLGQSCAADLEGWPAEYRAAMQGLGDYVRKNRFPGVVLGMSGGID
CCEEEEECCCHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
SAITAAIAADALGPDKVWCVMMPSKYTSNDSLEDAKACAQALGCRYDTINIHPGVEALDT
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHH
MLGDVFAGKKADITEENIQSRLRAVTLMALSNKFGQMVVTTGNKSEMAVGYATLYGDMCG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHCC
GYNALKDFYKTEVFELSKWRNAVHPKDALAPAGEVIPERIITKPPSAELREDQKDEDSLP
CHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCC
PYEVLDDILRGLVDLEEDVDDILARGHDAATVRRIEHLVYIAEYKRRQAPPGVKVGGKNF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
GRDRRYPITNRFRDD
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]