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The map label for this gene is gltX2

Identifier: 114799057

GI number: 114799057

Start: 2732741

End: 2734078

Strand: Direct

Name: gltX2

Synonym: HNE_2579

Alternate gene names: 114799057

Gene position: 2732741-2734078 (Clockwise)

Preceding gene: 114797585

Following gene: 114798784

Centisome position: 73.76

GC content: 63.68

Gene sequence:

>1338_bases
ATGACCCATCCTGTTGTTCGCTTTGCGCCCTCGCCCACTGGCCGGCTTCATGTCGGCAATGTGCGTACCGCCCTGATTAA
CTGGATGTTTTCACGCGGGCAGCAGGGTAAATTCATACTGCGCATCGATGACACGGACCTTGAACGCTCCACCGCCGAGC
ATGAGGAGGCCCTGAAAGTCGACCTCACCTGGCTCGGATTGACCTGGGATGACAGCTTCAGCCAGTCGCACCGTTTCGCA
AACTATGACGCCGCGGCCGACAAGCTGCGCGCCCTGCACCTGCTCTACCCCTGCTACGAGACGGCCGAGGAACTCGACGT
CAAACGCAAGATCGCCCAGACGCGCGGCCGCCCCCCGGTGTATGACCGCGCCGCTCTCAGCCTTACGGCGCAGGACCGCG
CTGATCTTGAAGCCAAAGGGCGCAAGCCTCACTGGCGCTTCAAATTGTCAGGCGAGCGGGTCGAATGGAACGATCTTGTG
CGCGGGCCCCAGAGCATCGACACGGCATCGGTCTCAGACCCGATCCTCATCCGCGAAGATGGCAGCTATCTCTATACGCT
CCCCTCGGTCGTCGATGACATAGAGGCGGGCATCACGCATGTCGTGCGCGGCGAAGACCATGTGACGAACTCCGGCGCGC
AGATCGAGATCTTCATGGCGCTCGGTGGCAAGGCGCCAGAGATGGCGCACACGCCGTTGCTGATCGGCGCGGATGGCGCA
GCGCTCTCCAAGCGCATCGGGTCGCTCTCGATGGGCGAGCTGCGCGCACGCGGTTATGAGCCGATGGCCATCTGCAGCCA
TCTCGCCAAGCTCGGCACGTCCGACAATATCGAGGCGCGCGCCACGCTGGAGCAGCTGTGCGAAGAGTTTTCCTTCTCCA
AAATTGGCCGCGCGCCCGCCCGCTTTGATGACAATGACCTCAACGCCCTCAATGCCGCGCTGGTACATGCCATGCCGTTT
GAGGCTGTCCGTGAACGGCTGATCACGCTGGATGCGCGCGCAGCCTCCGAGCCGTTCTGGCTGGCCGTGCGCGAGAACTG
CACCTTCGTGGCCGATGCGATCTCCTGGGTGGATATGGTCTATGGATCACCCGCCCCCTTAGTCGCCGAGGAAGACCGTG
AGTTCATCTCGGGCGCGGCTATGTACCTTCCGGAAGGCGAACTGACCACCGAGAGCTGGAGCGCGTGGACCAATGCCCTG
AAAGCCGCCACGGGCCGCAAGGGCCGGGGCCTGTTCATGCCCCTGCGCAAGGCCCTCACCGGCGCCGAGCACGGCCCGGA
AATGAGCGCCGTGCTGCCCCTCATCGGCCGCGAGAAAGTGCTGCAGCGTCTCAGCTGA

Upstream 100 bases:

>100_bases
GAGGTGAACCACAACAATAGCAAGAATATGACCGTGACGCTTGGCTTTTGCCGCGATTTGATGTCTCAAGCCGCTTCCCG
GAACGAGAAAGCCGCCGACC

Downstream 100 bases:

>100_bases
CCGCTCAATCGCAGCTGGAGTCCGTCTCGCAGGAAACGCTGGAGCCAGCGCGCCCCTCTGCCAGATCACGCACCCAGTCT
GAGAGCCGCACGCCGGCTGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTHPVVRFAPSPTGRLHVGNVRTALINWMFSRGQQGKFILRIDDTDLERSTAEHEEALKVDLTWLGLTWDDSFSQSHRFA
NYDAAADKLRALHLLYPCYETAEELDVKRKIAQTRGRPPVYDRAALSLTAQDRADLEAKGRKPHWRFKLSGERVEWNDLV
RGPQSIDTASVSDPILIREDGSYLYTLPSVVDDIEAGITHVVRGEDHVTNSGAQIEIFMALGGKAPEMAHTPLLIGADGA
ALSKRIGSLSMGELRARGYEPMAICSHLAKLGTSDNIEARATLEQLCEEFSFSKIGRAPARFDDNDLNALNAALVHAMPF
EAVRERLITLDARAASEPFWLAVRENCTFVADAISWVDMVYGSPAPLVAEEDREFISGAAMYLPEGELTTESWSAWTNAL
KAATGRKGRGLFMPLRKALTGAEHGPEMSAVLPLIGREKVLQRLS

Sequences:

>Translated_445_residues
MTHPVVRFAPSPTGRLHVGNVRTALINWMFSRGQQGKFILRIDDTDLERSTAEHEEALKVDLTWLGLTWDDSFSQSHRFA
NYDAAADKLRALHLLYPCYETAEELDVKRKIAQTRGRPPVYDRAALSLTAQDRADLEAKGRKPHWRFKLSGERVEWNDLV
RGPQSIDTASVSDPILIREDGSYLYTLPSVVDDIEAGITHVVRGEDHVTNSGAQIEIFMALGGKAPEMAHTPLLIGADGA
ALSKRIGSLSMGELRARGYEPMAICSHLAKLGTSDNIEARATLEQLCEEFSFSKIGRAPARFDDNDLNALNAALVHAMPF
EAVRERLITLDARAASEPFWLAVRENCTFVADAISWVDMVYGSPAPLVAEEDREFISGAAMYLPEGELTTESWSAWTNAL
KAATGRKGRGLFMPLRKALTGAEHGPEMSAVLPLIGREKVLQRLS
>Mature_444_residues
THPVVRFAPSPTGRLHVGNVRTALINWMFSRGQQGKFILRIDDTDLERSTAEHEEALKVDLTWLGLTWDDSFSQSHRFAN
YDAAADKLRALHLLYPCYETAEELDVKRKIAQTRGRPPVYDRAALSLTAQDRADLEAKGRKPHWRFKLSGERVEWNDLVR
GPQSIDTASVSDPILIREDGSYLYTLPSVVDDIEAGITHVVRGEDHVTNSGAQIEIFMALGGKAPEMAHTPLLIGADGAA
LSKRIGSLSMGELRARGYEPMAICSHLAKLGTSDNIEARATLEQLCEEFSFSKIGRAPARFDDNDLNALNAALVHAMPFE
AVRERLITLDARAASEPFWLAVRENCTFVADAISWVDMVYGSPAPLVAEEDREFISGAAMYLPEGELTTESWSAWTNALK
AATGRKGRGLFMPLRKALTGAEHGPEMSAVLPLIGREKVLQRLS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=481, Percent_Identity=29.3139293139293, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=33.0453563714903, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=32.7935222672065, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71993394, Length=471, Percent_Identity=27.8131634819533, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324540, Length=326, Percent_Identity=31.9018404907975, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24665321, Length=480, Percent_Identity=28.5416666666667, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_HYPNA (Q0BZ24)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761269.1
- ProteinModelPortal:   Q0BZ24
- SMR:   Q0BZ24
- STRING:   Q0BZ24
- GeneID:   4288956
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2579
- NMPDR:   fig|228405.5.peg.2478
- TIGR:   HNE_2579
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   HNEP81032:HNE_2579-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 49179; Mature: 49048

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPVVRFAPSPTGRLHVGNVRTALINWMFSRGQQGKFILRIDDTDLERSTAEHEEALKV
CCCCEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHEEE
DLTWLGLTWDDSFSQSHRFANYDAAADKLRALHLLYPCYETAEELDVKRKIAQTRGRPPV
EEEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YDRAALSLTAQDRADLEAKGRKPHWRFKLSGERVEWNDLVRGPQSIDTASVSDPILIRED
CCCCEEEEECCCCCCHHCCCCCCCEEEEECCCEECHHHHHCCCCCCCCCCCCCCEEEEEC
GSYLYTLPSVVDDIEAGITHVVRGEDHVTNSGAQIEIFMALGGKAPEMAHTPLLIGADGA
CCEEEECHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCH
ALSKRIGSLSMGELRARGYEPMAICSHLAKLGTSDNIEARATLEQLCEEFSFSKIGRAPA
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCC
RFDDNDLNALNAALVHAMPFEAVRERLITLDARAASEPFWLAVRENCTFVADAISWVDMV
CCCCCCHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHH
YGSPAPLVAEEDREFISGAAMYLPEGELTTESWSAWTNALKAATGRKGRGLFMPLRKALT
HCCCCCCCCCCCHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GAEHGPEMSAVLPLIGREKVLQRLS
CCCCCCCHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
THPVVRFAPSPTGRLHVGNVRTALINWMFSRGQQGKFILRIDDTDLERSTAEHEEALKV
CCCEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHEEE
DLTWLGLTWDDSFSQSHRFANYDAAADKLRALHLLYPCYETAEELDVKRKIAQTRGRPPV
EEEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YDRAALSLTAQDRADLEAKGRKPHWRFKLSGERVEWNDLVRGPQSIDTASVSDPILIRED
CCCCEEEEECCCCCCHHCCCCCCCEEEEECCCEECHHHHHCCCCCCCCCCCCCCEEEEEC
GSYLYTLPSVVDDIEAGITHVVRGEDHVTNSGAQIEIFMALGGKAPEMAHTPLLIGADGA
CCEEEECHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCH
ALSKRIGSLSMGELRARGYEPMAICSHLAKLGTSDNIEARATLEQLCEEFSFSKIGRAPA
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCC
RFDDNDLNALNAALVHAMPFEAVRERLITLDARAASEPFWLAVRENCTFVADAISWVDMV
CCCCCCHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHH
YGSPAPLVAEEDREFISGAAMYLPEGELTTESWSAWTNALKAATGRKGRGLFMPLRKALT
HCCCCCCCCCCCHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
GAEHGPEMSAVLPLIGREKVLQRLS
CCCCCCCHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA