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The map label for this gene is hisI

Identifier: 114797571

GI number: 114797571

Start: 3521179

End: 3521574

Strand: Direct

Name: hisI

Synonym: HNE_3376

Alternate gene names: 114797571

Gene position: 3521179-3521574 (Clockwise)

Preceding gene: 114797976

Following gene: 114799156

Centisome position: 95.04

GC content: 64.9

Gene sequence:

>396_bases
ATGACGCCGTTCCCGCCCCCCCTCTCCGGCAGTGCCCAGGATGAAACGCCGGAGCTTCGCCCGCGCTTTGACGAGAACGG
CCTCATCGCCGCCATCGCGCAGGATGCGGGCACCGGTGAGGTGCTGATGCTCGCCTGGATGAATGCCGAAGCGCTACAGA
AAACGATAGAGACCGGCCGGGCCACCTACTGGTCGCGCTCACGCGGGGCGCTTTGGGTGAAGGGTGAGACCTCCGGCCAC
ACGCAGGAAGTCATCGAACTGCGCGTCGATTGCGATCAGGACGCCGTACTGCTGAAAGTGCGCCAGACAGGCGGGGCCTG
CCACACCCACCGGGAAAGCTGCTTCTATCGGCGCATCGAAGGCGGGGTGCTATCCTTCACCGGCAAGGCAGATTAG

Upstream 100 bases:

>100_bases
TTTCCCTCCAGTCTAGACGCCTTTCCGCCCAATGGGCAGACGCAAATCTGCAGCGGCGCCTTGCTTGACACCGCCCCCGC
CTTGGGCGACCGCTGCGGGC

Downstream 100 bases:

>100_bases
GCTCCTCCAAAACCGGAGGAACCCCATGCGTCTGCGACACACCCTGAAACCGCTGCTGCTCGCCAGCTCTGCTGCTGTCA
TTCTCGCGGCCTGCGCGCCC

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MTPFPPPLSGSAQDETPELRPRFDENGLIAAIAQDAGTGEVLMLAWMNAEALQKTIETGRATYWSRSRGALWVKGETSGH
TQEVIELRVDCDQDAVLLKVRQTGGACHTHRESCFYRRIEGGVLSFTGKAD

Sequences:

>Translated_131_residues
MTPFPPPLSGSAQDETPELRPRFDENGLIAAIAQDAGTGEVLMLAWMNAEALQKTIETGRATYWSRSRGALWVKGETSGH
TQEVIELRVDCDQDAVLLKVRQTGGACHTHRESCFYRRIEGGVLSFTGKAD
>Mature_130_residues
TPFPPPLSGSAQDETPELRPRFDENGLIAAIAQDAGTGEVLMLAWMNAEALQKTIETGRATYWSRSRGALWVKGETSGHT
QEVIELRVDCDQDAVLLKVRQTGGACHTHRESCFYRRIEGGVLSFTGKAD

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=90, Percent_Identity=47.7777777777778, Blast_Score=95, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319819, Length=75, Percent_Identity=42.6666666666667, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_HYPNA (Q0BWU4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762049.1
- ProteinModelPortal:   Q0BWU4
- SMR:   Q0BWU4
- STRING:   Q0BWU4
- GeneID:   4287289
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3376
- NMPDR:   fig|228405.5.peg.3231
- TIGR:   HNE_3376
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- BioCyc:   HNEP81032:HNE_3376-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 14378; Mature: 14247

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFPPPLSGSAQDETPELRPRFDENGLIAAIAQDAGTGEVLMLAWMNAEALQKTIETGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHCC
ATYWSRSRGALWVKGETSGHTQEVIELRVDCDQDAVLLKVRQTGGACHTHRESCFYRRIE
CEEEECCCCEEEEECCCCCCHHEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHC
GGVLSFTGKAD
CCEEEECCCCC
>Mature Secondary Structure 
TPFPPPLSGSAQDETPELRPRFDENGLIAAIAQDAGTGEVLMLAWMNAEALQKTIETGR
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHCC
ATYWSRSRGALWVKGETSGHTQEVIELRVDCDQDAVLLKVRQTGGACHTHRESCFYRRIE
CEEEECCCCEEEEECCCCCCHHEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHC
GGVLSFTGKAD
CCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA