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The map label for this gene is purU [H]

Identifier: 114797552

GI number: 114797552

Start: 799594

End: 800451

Strand: Reverse

Name: purU [H]

Synonym: HNE_0787

Alternate gene names: 114797552

Gene position: 800451-799594 (Counterclockwise)

Preceding gene: 114798407

Following gene: 114798411

Centisome position: 21.6

GC content: 62.35

Gene sequence:

>858_bases
TTGTCGCAGACCTTCATCCTTACCCTGAGTTGCCCGGACAGGGTTGGCCTCGTGGCCAGCCTTGCACGGCTCATGGAGGA
GCATGGCTGCTTCATCGTCACCTCGCGCACCTTCGGTGATCCGCAGACGAACCGTTTCTTTGCCCGGCTGGTGTTCAGCC
CGCCGGGCAATGGCGGTTCGCTCGACGCCGTGAAGGCCGCGATGGCGCCGCTCGCCGCAGAGATCGAAGCCGACTGGCAG
ATCCGCTCGTCGGACGAAAAGCTGCGCACCCTGCTGCTGGTCTCGAAGTCAGACCATTGCGCCAACACGCTGCTCTACGC
GGCGCGGCGCCATGAACTGCCCATCGAGATTACCGGCATCGTCTCCAATCATGACAGCCTCAAGCCGGCTTTTGCCCATT
GGGGCCTGCCCTGGTTCCATGTCCCGGTCACGGCCGCATCCAAGCCCGACGCCGAAGCGCTCCTCTACAGTATCATTGAA
GAGACCCGCTCCGAACTGGTCGTCCTTGCACGCTACATGCAGGTGCTCAGCGAGGAGGCCTGCCGCCGTCTTGAAGGCCG
CGTCATCAACATCCACCATTCCTTCCTGCCCGGTTTCAAGGGCGCCCAGCCCTATCACCAGGCGCATGCCCGCGGCGTAA
AAGTCATCGGCGCTACGGCCCATTATGTCACCGCCGATCTCGATGAAGGCCCGATCATCACCCAGGCCACCGAAACCATC
GACCACACATTCACGCCAGAAGACATGGTCGAAACCGGCCGCCACATCGAAGGCATTGCCCTTCTGCGGGCGGTAAAAGC
CCATGCCGAGCACCGCATTTTCCTGAATTCCGGCCGCACAGTCGTGTTCGCCCGGTAA

Upstream 100 bases:

>100_bases
ACCAAGCTGTCGGGTGAACAGGCCGCGTATATCGGCGTGGAACAGACCGGCCCGTTCAAGCCGGAACATTACCGCTACTG
ATCCGGCAGCGGGGACAGCC

Downstream 100 bases:

>100_bases
GCCATTCCAAGAGGAGCCAAGAATGCCCCTGAAGTCCCATGAGTTCACCAGCGAGAGCGTCGCCGAAGGCCATCCCGACA
AGGTTGCCGACCAGATTTCC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSQTFILTLSCPDRVGLVASLARLMEEHGCFIVTSRTFGDPQTNRFFARLVFSPPGNGGSLDAVKAAMAPLAAEIEADWQ
IRSSDEKLRTLLLVSKSDHCANTLLYAARRHELPIEITGIVSNHDSLKPAFAHWGLPWFHVPVTAASKPDAEALLYSIIE
ETRSELVVLARYMQVLSEEACRRLEGRVINIHHSFLPGFKGAQPYHQAHARGVKVIGATAHYVTADLDEGPIITQATETI
DHTFTPEDMVETGRHIEGIALLRAVKAHAEHRIFLNSGRTVVFAR

Sequences:

>Translated_285_residues
MSQTFILTLSCPDRVGLVASLARLMEEHGCFIVTSRTFGDPQTNRFFARLVFSPPGNGGSLDAVKAAMAPLAAEIEADWQ
IRSSDEKLRTLLLVSKSDHCANTLLYAARRHELPIEITGIVSNHDSLKPAFAHWGLPWFHVPVTAASKPDAEALLYSIIE
ETRSELVVLARYMQVLSEEACRRLEGRVINIHHSFLPGFKGAQPYHQAHARGVKVIGATAHYVTADLDEGPIITQATETI
DHTFTPEDMVETGRHIEGIALLRAVKAHAEHRIFLNSGRTVVFAR
>Mature_284_residues
SQTFILTLSCPDRVGLVASLARLMEEHGCFIVTSRTFGDPQTNRFFARLVFSPPGNGGSLDAVKAAMAPLAAEIEADWQI
RSSDEKLRTLLLVSKSDHCANTLLYAARRHELPIEITGIVSNHDSLKPAFAHWGLPWFHVPVTAASKPDAEALLYSIIEE
TRSELVVLARYMQVLSEEACRRLEGRVINIHHSFLPGFKGAQPYHQAHARGVKVIGATAHYVTADLDEGPIITQATETID
HTFTPEDMVETGRHIEGIALLRAVKAHAEHRIFLNSGRTVVFAR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=117, Percent_Identity=35.042735042735, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI209869995, Length=117, Percent_Identity=35.042735042735, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI209869993, Length=117, Percent_Identity=35.042735042735, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=38.0782918149466, Blast_Score=197, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17567511, Length=172, Percent_Identity=30.8139534883721, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31477; Mature: 31346

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTFILTLSCPDRVGLVASLARLMEEHGCFIVTSRTFGDPQTNRFFARLVFSPPGNGGS
CCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCC
LDAVKAAMAPLAAEIEADWQIRSSDEKLRTLLLVSKSDHCANTLLYAARRHELPIEITGI
HHHHHHHHHHHHHHCCCCEEEECCHHHEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEE
VSNHDSLKPAFAHWGLPWFHVPVTAASKPDAEALLYSIIEETRSELVVLARYMQVLSEEA
ECCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRRLEGRVINIHHSFLPGFKGAQPYHQAHARGVKVIGATAHYVTADLDEGPIITQATETI
HHHHCCEEEEEEHHHCCCCCCCCCHHHHHHCCEEEEEEEEEEEEEECCCCCEEEHHHHHH
DHTFTPEDMVETGRHIEGIALLRAVKAHAEHRIFLNSGRTVVFAR
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEC
>Mature Secondary Structure 
SQTFILTLSCPDRVGLVASLARLMEEHGCFIVTSRTFGDPQTNRFFARLVFSPPGNGGS
CCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCC
LDAVKAAMAPLAAEIEADWQIRSSDEKLRTLLLVSKSDHCANTLLYAARRHELPIEITGI
HHHHHHHHHHHHHHCCCCEEEECCHHHEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEE
VSNHDSLKPAFAHWGLPWFHVPVTAASKPDAEALLYSIIEETRSELVVLARYMQVLSEEA
ECCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRRLEGRVINIHHSFLPGFKGAQPYHQAHARGVKVIGATAHYVTADLDEGPIITQATETI
HHHHCCEEEEEEHHHCCCCCCCCCHHHHHHCCEEEEEEEEEEEEEECCCCCEEEHHHHHH
DHTFTPEDMVETGRHIEGIALLRAVKAHAEHRIFLNSGRTVVFAR
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]