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The map label for this gene is nadR [H]

Identifier: 114797512

GI number: 114797512

Start: 1183875

End: 1184483

Strand: Reverse

Name: nadR [H]

Synonym: HNE_1175

Alternate gene names: 114797512

Gene position: 1184483-1183875 (Counterclockwise)

Preceding gene: 114798743

Following gene: 114800352

Centisome position: 31.97

GC content: 64.7

Gene sequence:

>609_bases
ATGGCGCAAATCGGTGAATGCATGACCTGCAACAAACCCCTGCGCGTCGTCCTGATCGGCGCCGAATCCACCGGCAAGAC
CACGCTCTGCATCGAGCTGGCCGCCCATTACAACACCGTCTGGGTCGCCGAATTCGGCCGGGAACTCTGGGAACAGAAAC
TCGCCGCCCAGTCCCTCAAGGGCGGCGACATTCCCGAATGGACCGATGAGGATTTCATCCACATTGCGCAGGAACAGCAG
CGCCGCGAAAACGAAGCCGCCACCCGCGCCAACGGTATCCTCTTCGCCGACACCAACGCCTTCGCCACCGCCACCTGGTA
CGAACGCTATGCCGGCAAGCGCCACCCTGAGGTGGACGCGATAGGCGACCGCGACCAGGTCGATCTCTACCTCATCCCCA
CCCCGGAAGTCCCCTTTGTGCAGGACGGTGTGCGCGACGGCGAGAAGATCCGCGGCTGGATGCACGACCGGTTCGTCGAG
CTGATCGAGAAGGCAGGTATTCCGTACACGCTCATTACCGGCCCCTGGGAAGGCCGCGTCGAGCAGGCCACCGCCGCCAT
CGACGCCCTCCTCGCCCGTTGCCAGGCCGAGGCTCTGCCCGCCCCATGA

Upstream 100 bases:

>100_bases
CCGACATTATCTACATCCCCGTCTGCCTGTTTAACGGCCTTTACCTGACGGCCCTTCTGTATGCGGTGTTTCTGGCGCTC
TCAGTCATGGGCCTGCTGGA

Downstream 100 bases:

>100_bases
GCGAAATCACGATCCGCCGCGCGCAGGCCCACGATGCCGAGCGTCTCGCCCATATTGGCGTGGCCACCTTCATCGACAGC
TATGTCTTCGACATCGACGG

Product: putative nicotinamide-nucleotide adenylyltransferase

Products: NA

Alternate protein names: Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAQIGECMTCNKPLRVVLIGAESTGKTTLCIELAAHYNTVWVAEFGRELWEQKLAAQSLKGGDIPEWTDEDFIHIAQEQQ
RRENEAATRANGILFADTNAFATATWYERYAGKRHPEVDAIGDRDQVDLYLIPTPEVPFVQDGVRDGEKIRGWMHDRFVE
LIEKAGIPYTLITGPWEGRVEQATAAIDALLARCQAEALPAP

Sequences:

>Translated_202_residues
MAQIGECMTCNKPLRVVLIGAESTGKTTLCIELAAHYNTVWVAEFGRELWEQKLAAQSLKGGDIPEWTDEDFIHIAQEQQ
RRENEAATRANGILFADTNAFATATWYERYAGKRHPEVDAIGDRDQVDLYLIPTPEVPFVQDGVRDGEKIRGWMHDRFVE
LIEKAGIPYTLITGPWEGRVEQATAAIDALLARCQAEALPAP
>Mature_201_residues
AQIGECMTCNKPLRVVLIGAESTGKTTLCIELAAHYNTVWVAEFGRELWEQKLAAQSLKGGDIPEWTDEDFIHIAQEQQR
RENEAATRANGILFADTNAFATATWYERYAGKRHPEVDAIGDRDQVDLYLIPTPEVPFVQDGVRDGEKIRGWMHDRFVEL
IEKAGIPYTLITGPWEGRVEQATAAIDALLARCQAEALPAP

Specific function: This enzyme has three activities:DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase

COG id: COG3172

COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082440, Length=181, Percent_Identity=30.3867403314917, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016429
- InterPro:   IPR004821
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR006417
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: =2.7.7.1; =2.7.1.22 [H]

Molecular weight: Translated: 22569; Mature: 22437

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIGECMTCNKPLRVVLIGAESTGKTTLCIELAAHYNTVWVAEFGRELWEQKLAAQSLK
CCCCCCCEECCCCEEEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHCC
GGDIPEWTDEDFIHIAQEQQRRENEAATRANGILFADTNAFATATWYERYAGKRHPEVDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEHHHHHHHHCCCCCCCCCC
IGDRDQVDLYLIPTPEVPFVQDGVRDGEKIRGWMHDRFVELIEKAGIPYTLITGPWEGRV
CCCCCCEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
EQATAAIDALLARCQAEALPAP
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AQIGECMTCNKPLRVVLIGAESTGKTTLCIELAAHYNTVWVAEFGRELWEQKLAAQSLK
CCCCCCEECCCCEEEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHCC
GGDIPEWTDEDFIHIAQEQQRRENEAATRANGILFADTNAFATATWYERYAGKRHPEVDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEHHHHHHHHCCCCCCCCCC
IGDRDQVDLYLIPTPEVPFVQDGVRDGEKIRGWMHDRFVELIEKAGIPYTLITGPWEGRV
CCCCCCEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
EQATAAIDALLARCQAEALPAP
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 1327967 [H]