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The map label for this gene is gph [H]

Identifier: 114797471

GI number: 114797471

Start: 715690

End: 716403

Strand: Reverse

Name: gph [H]

Synonym: HNE_0704

Alternate gene names: 114797471

Gene position: 716403-715690 (Counterclockwise)

Preceding gene: 114800256

Following gene: 114797159

Centisome position: 19.34

GC content: 63.17

Gene sequence:

>714_bases
ATGGCTGAAAACGGCAGGTTTGACGGCTGGACGGTCGCGTTCGATCTGGACGGTACGCTGGTGGATACCGCGCCCGACCT
TCTGGGCGCGCTCAACCATGTGCTCACCGATGCGGGCCTCGAGCCTGTCGACCTGCCCACCATCGCGACGCTGATCGGCA
ATGGCGCCCGCGCCATGATGGAAAAGGGATTCGGGGTTCAGGGCGTTACCTTGCCAGCTGCGGAAATGGACGCTGCTTTC
GAGCGGTTCATCGCCTATTATATTGCCAACATCGCGGTAGATTCGCGGCCCTTCGAGGGCTGCACCGAGGCGCTCGACGC
GCTGCTGGATGCGGGGGCCACACTCTGCGTGTGCACCAACAAGCGGCAGGATCTCAGCGAGCGTCTGATCGGCGAACTTG
GCCTCACGGACCGGTTTGCCGCCATCCTCGGCGCAGACCGGGCAACCAACCGCAAGCCCCACCAGGACCATGTGTTCGAG
GCCGTTCGCGCCGCAGGCGGCACGCCGGAGCGCGCGCTGTTCGTGGGGGATAGCCGAACAGATGAAAGGGCAGCCCGGAA
TGCCGGGCTGCCCTTCCTGTTTGTGACCTTTGGCTACGAAGCGGAAACGCCTGAGATGATCGCGCCCGATGGCATCATCG
GTCACTATGCTCAGCTGTTTGACGCTTTGTCAGTCCTTAGGGATCAGGCCATCGCCTCATTGCGCGGACGGTAA

Upstream 100 bases:

>100_bases
TGTTTGCGGGTGTCGGTCAATGCGGGCTCCCTCTCGCTTTCCTCTGCCCCATCGCCTAGACGAAACCCCGCATCTTGGCG
AGGGCGAAGGAGGGTTCCAG

Downstream 100 bases:

>100_bases
GTCGGGCGGGAAGAGCCCACTTCACCGGCAGCACGGCGGCGCGGCATGCCATTGACCATATTGGAACGCACGTCACAACG
CGCGCTCCGCATCGCAGGAA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAENGRFDGWTVAFDLDGTLVDTAPDLLGALNHVLTDAGLEPVDLPTIATLIGNGARAMMEKGFGVQGVTLPAAEMDAAF
ERFIAYYIANIAVDSRPFEGCTEALDALLDAGATLCVCTNKRQDLSERLIGELGLTDRFAAILGADRATNRKPHQDHVFE
AVRAAGGTPERALFVGDSRTDERAARNAGLPFLFVTFGYEAETPEMIAPDGIIGHYAQLFDALSVLRDQAIASLRGR

Sequences:

>Translated_237_residues
MAENGRFDGWTVAFDLDGTLVDTAPDLLGALNHVLTDAGLEPVDLPTIATLIGNGARAMMEKGFGVQGVTLPAAEMDAAF
ERFIAYYIANIAVDSRPFEGCTEALDALLDAGATLCVCTNKRQDLSERLIGELGLTDRFAAILGADRATNRKPHQDHVFE
AVRAAGGTPERALFVGDSRTDERAARNAGLPFLFVTFGYEAETPEMIAPDGIIGHYAQLFDALSVLRDQAIASLRGR
>Mature_236_residues
AENGRFDGWTVAFDLDGTLVDTAPDLLGALNHVLTDAGLEPVDLPTIATLIGNGARAMMEKGFGVQGVTLPAAEMDAAFE
RFIAYYIANIAVDSRPFEGCTEALDALLDAGATLCVCTNKRQDLSERLIGELGLTDRFAAILGADRATNRKPHQDHVFEA
VRAAGGTPERALFVGDSRTDERAARNAGLPFLFVTFGYEAETPEMIAPDGIIGHYAQLFDALSVLRDQAIASLRGR

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=32.7586206896552, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25373; Mature: 25242

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENGRFDGWTVAFDLDGTLVDTAPDLLGALNHVLTDAGLEPVDLPTIATLIGNGARAMM
CCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
EKGFGVQGVTLPAAEMDAAFERFIAYYIANIAVDSRPFEGCTEALDALLDAGATLCVCTN
HCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECC
KRQDLSERLIGELGLTDRFAAILGADRATNRKPHQDHVFEAVRAAGGTPERALFVGDSRT
CHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
DERAARNAGLPFLFVTFGYEAETPEMIAPDGIIGHYAQLFDALSVLRDQAIASLRGR
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AENGRFDGWTVAFDLDGTLVDTAPDLLGALNHVLTDAGLEPVDLPTIATLIGNGARAMM
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
EKGFGVQGVTLPAAEMDAAFERFIAYYIANIAVDSRPFEGCTEALDALLDAGATLCVCTN
HCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECC
KRQDLSERLIGELGLTDRFAAILGADRATNRKPHQDHVFEAVRAAGGTPERALFVGDSRT
CHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
DERAARNAGLPFLFVTFGYEAETPEMIAPDGIIGHYAQLFDALSVLRDQAIASLRGR
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]