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The map label for this gene is ttcA

Identifier: 114797440

GI number: 114797440

Start: 2931918

End: 2932805

Strand: Direct

Name: ttcA

Synonym: HNE_2769

Alternate gene names: 114797440

Gene position: 2931918-2932805 (Clockwise)

Preceding gene: 114797503

Following gene: 114799162

Centisome position: 79.13

GC content: 62.5

Gene sequence:

>888_bases
ATGGCTGACACCTCCCTCTCCCCCGTTTTGCCGCTGTTTGCGAACACGCCGTCGAGCGTCAGCTTCAAGAAGCTGCGCAA
ACGGCTGGTGCGCGGGGCGCTGGAGGTGATCGACACGTACGGTCTGGTGGACCGCCGCGCCATCGAAACCGGCGAAAAGC
CACACCCTAAATGGCTGGTCTGTCTCTCCGGCGGCAAGGACTCCTATGGCCTTCTGGCTGTGCTGCTGGATCTGCAATGG
CAGGGCGCGCTGCCCGTAGACCTCATCGCCTGTAATCTCGATCAGGGCCAGCCGGGCTTTCCCAAGCATATCCTGCCAGA
GTGGCTGACGCAGAACGGTGTCACCCACAAGATCATCACCGAGGACACCTATTCCATCGTGACCGACAAGGTGCCCGAAG
GGCGCACCTATTGTTCGATGTGTTCACGCCTGCGCCGGGGCATTCTTTACCGCGTCGCGCGCGAGGAAGGCTGTGAGGCC
ATCGTGCTCGGCCACCACCGGGATGACGCGCTCGCCACCTTCATGATGAACCTCATCCATGGCGGGCGCCTGGCCGCGAT
GCCGCCCAAGCTGCTGAACGATGACGGCGATGTGCAGGTGTTCCGCCCGCTGATCACTGCTGCGGAAGATGACCTCGCCA
AATTTGCCGAGGCGATGGAATTTCCCATCATCCCCTGCAATCTCTGCGGCAGCCAGGATGGCCTTCAGCGCGTGGTGATG
AACCGCATGCTGGACGAGTGGGAGCGCAAGAAGCCGGGCACGCGGCAGGTGATGGCCAAGGCACTTACCAATGTGCGCCC
CAGCCATCTGCATGATCCGCGCGTCTTCGACTTTGCCGGCCTGATGCTCGGCGGCAAGGGCGAGGACGACCCGAACGTTC
CTTTCTAG

Upstream 100 bases:

>100_bases
ACAAGGGCCGGGCAAAAAAAATCGCCCAGGCCATCCGTGCGCTGCAGGACTTTGACGCCTGGTTGGCAGGTGAAGCCGTG
GTGGCTGCGGAGTAAAACAG

Downstream 100 bases:

>100_bases
TTACACCCTCTCTTGCACCGGCGGCCAAGCAATGCCGGCCTCCGGGCGGTGGGCGGGATAGGCCGGTGCCTCGCGGTACT
TTCTCACCTCATTGCGGGCA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MADTSLSPVLPLFANTPSSVSFKKLRKRLVRGALEVIDTYGLVDRRAIETGEKPHPKWLVCLSGGKDSYGLLAVLLDLQW
QGALPVDLIACNLDQGQPGFPKHILPEWLTQNGVTHKIITEDTYSIVTDKVPEGRTYCSMCSRLRRGILYRVAREEGCEA
IVLGHHRDDALATFMMNLIHGGRLAAMPPKLLNDDGDVQVFRPLITAAEDDLAKFAEAMEFPIIPCNLCGSQDGLQRVVM
NRMLDEWERKKPGTRQVMAKALTNVRPSHLHDPRVFDFAGLMLGGKGEDDPNVPF

Sequences:

>Translated_295_residues
MADTSLSPVLPLFANTPSSVSFKKLRKRLVRGALEVIDTYGLVDRRAIETGEKPHPKWLVCLSGGKDSYGLLAVLLDLQW
QGALPVDLIACNLDQGQPGFPKHILPEWLTQNGVTHKIITEDTYSIVTDKVPEGRTYCSMCSRLRRGILYRVAREEGCEA
IVLGHHRDDALATFMMNLIHGGRLAAMPPKLLNDDGDVQVFRPLITAAEDDLAKFAEAMEFPIIPCNLCGSQDGLQRVVM
NRMLDEWERKKPGTRQVMAKALTNVRPSHLHDPRVFDFAGLMLGGKGEDDPNVPF
>Mature_294_residues
ADTSLSPVLPLFANTPSSVSFKKLRKRLVRGALEVIDTYGLVDRRAIETGEKPHPKWLVCLSGGKDSYGLLAVLLDLQWQ
GALPVDLIACNLDQGQPGFPKHILPEWLTQNGVTHKIITEDTYSIVTDKVPEGRTYCSMCSRLRRGILYRVAREEGCEAI
VLGHHRDDALATFMMNLIHGGRLAAMPPKLLNDDGDVQVFRPLITAAEDDLAKFAEAMEFPIIPCNLCGSQDGLQRVVMN
RMLDEWERKKPGTRQVMAKALTNVRPSHLHDPRVFDFAGLMLGGKGEDDPNVPF

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=266, Percent_Identity=51.1278195488722, Blast_Score=281, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI193211090, Length=212, Percent_Identity=29.7169811320755, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_HYPNA (Q0BYJ5)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761448.1
- ProteinModelPortal:   Q0BYJ5
- STRING:   Q0BYJ5
- GeneID:   4287177
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2769
- NMPDR:   fig|228405.5.peg.2655
- TIGR:   HNE_2769
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   Q0BYJ5
- ProtClustDB:   PRK10696
- BioCyc:   HNEP81032:HNE_2769-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 32768; Mature: 32637

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTSLSPVLPLFANTPSSVSFKKLRKRLVRGALEVIDTYGLVDRRAIETGEKPHPKWLV
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEE
CLSGGKDSYGLLAVLLDLQWQGALPVDLIACNLDQGQPGFPKHILPEWLTQNGVTHKIIT
EECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEC
EDTYSIVTDKVPEGRTYCSMCSRLRRGILYRVAREEGCEAIVLGHHRDDALATFMMNLIH
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GGRLAAMPPKLLNDDGDVQVFRPLITAAEDDLAKFAEAMEFPIIPCNLCGSQDGLQRVVM
CCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCCCHHHHHHHHH
NRMLDEWERKKPGTRQVMAKALTNVRPSHLHDPRVFDFAGLMLGGKGEDDPNVPF
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure 
ADTSLSPVLPLFANTPSSVSFKKLRKRLVRGALEVIDTYGLVDRRAIETGEKPHPKWLV
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEE
CLSGGKDSYGLLAVLLDLQWQGALPVDLIACNLDQGQPGFPKHILPEWLTQNGVTHKIIT
EECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEC
EDTYSIVTDKVPEGRTYCSMCSRLRRGILYRVAREEGCEAIVLGHHRDDALATFMMNLIH
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GGRLAAMPPKLLNDDGDVQVFRPLITAAEDDLAKFAEAMEFPIIPCNLCGSQDGLQRVVM
CCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCCCHHHHHHHHH
NRMLDEWERKKPGTRQVMAKALTNVRPSHLHDPRVFDFAGLMLGGKGEDDPNVPF
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA