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The map label for this gene is rpoH [H]

Identifier: 114797392

GI number: 114797392

Start: 324491

End: 325387

Strand: Reverse

Name: rpoH [H]

Synonym: HNE_0330

Alternate gene names: 114797392

Gene position: 325387-324491 (Counterclockwise)

Preceding gene: 114798141

Following gene: 114800064

Centisome position: 8.78

GC content: 60.65

Gene sequence:

>897_bases
ATGGCGAATGCGAACGCAAAAAGCTCGGTCGCGCTAAGCCCCGAACAGGGCATGAGCCGCTATCTGAGCGAAATCCGCAA
ATTCCCGATGCTCGAGAAGAATGAGGAATTCATGCTGGCCCGCCGGTGGCACGAGCATGAAGACACCGAAGCGGCCGAGA
AGATGGTGACCTCGCACCTGCGCCTCGTGGCGAAGATTGCGATGGGCTATCGCGGCTATGGCCTGCCGATGGCCGAGGTT
ATCTCAGAGGGCAATGTGGGCCTGATGCAGGCCGTGAAGAAATTCGACCCCGACAAGGGCTTCCGCCTCGCGACCTATGC
GATGTGGTGGATTCGCGCCGCGATCCAGGAATACATCCTGCGCAGCTGGAGCCTTGTGAAACTTGGCACCACGGCGGCCC
AGAAGAAGCTGTTCTTCAACCTGCGCCGCCTGAAGGGCGAAATGCAGGCGCTGGAGGAAGGCGACCTGAAACCCGAACAG
GCCCGCCTGATCGCCGAGAAGCTGAATGTGACCGAGACCGAAGTTTATTCGATGAACGGACGCATGAGCGGTTCGGACGC
CTCGCTGAACGTGCCGATGGGCACCGATGGCGATATGGAATGGCAGGACTGGCTGGCCGATGACGAGCCGGGCACAGCCG
AAACCTTCGCCAACAGCCAGGAACTTGACGCACGCATGACCCTGCTGACGCAGGCGATGGAAGACCTTTCCGAGCGTGAG
CGCCACATCCTGACCGAGCGGCGCCTGGTGGACGAGCCCAAGACGCTGGAAGAGCTTTCCGAGCAGTATAATGTCAGCCG
CGAGCGTATCCGGCAGATTGAAGTGCGCGCGTTTGAAAAGCTGCAGAAGGCGATGAAGCGCATGGCCAAGGAACAGGGCC
TCCCTATGGGGGCCTGA

Upstream 100 bases:

>100_bases
TGACGACGCTGCCGCTGAGCGGGGCTAAGCTCCAGGTGAGCTCTAAAGACAGGAGCAGGCCGCCCGAAGAGGGGGCCGGT
TCCGAACAGGAGGACTGAAC

Downstream 100 bases:

>100_bases
TCCTTTGGCTTAGCCGCCTTCCCCTGGAACCTCCCTCCTGCTCCGGCGTTGGTCTGGAACAGGTAGGAGGGCCAAGGCCA
TGTCATTCAAGATCGTTATT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MANANAKSSVALSPEQGMSRYLSEIRKFPMLEKNEEFMLARRWHEHEDTEAAEKMVTSHLRLVAKIAMGYRGYGLPMAEV
ISEGNVGLMQAVKKFDPDKGFRLATYAMWWIRAAIQEYILRSWSLVKLGTTAAQKKLFFNLRRLKGEMQALEEGDLKPEQ
ARLIAEKLNVTETEVYSMNGRMSGSDASLNVPMGTDGDMEWQDWLADDEPGTAETFANSQELDARMTLLTQAMEDLSERE
RHILTERRLVDEPKTLEELSEQYNVSRERIRQIEVRAFEKLQKAMKRMAKEQGLPMGA

Sequences:

>Translated_298_residues
MANANAKSSVALSPEQGMSRYLSEIRKFPMLEKNEEFMLARRWHEHEDTEAAEKMVTSHLRLVAKIAMGYRGYGLPMAEV
ISEGNVGLMQAVKKFDPDKGFRLATYAMWWIRAAIQEYILRSWSLVKLGTTAAQKKLFFNLRRLKGEMQALEEGDLKPEQ
ARLIAEKLNVTETEVYSMNGRMSGSDASLNVPMGTDGDMEWQDWLADDEPGTAETFANSQELDARMTLLTQAMEDLSERE
RHILTERRLVDEPKTLEELSEQYNVSRERIRQIEVRAFEKLQKAMKRMAKEQGLPMGA
>Mature_297_residues
ANANAKSSVALSPEQGMSRYLSEIRKFPMLEKNEEFMLARRWHEHEDTEAAEKMVTSHLRLVAKIAMGYRGYGLPMAEVI
SEGNVGLMQAVKKFDPDKGFRLATYAMWWIRAAIQEYILRSWSLVKLGTTAAQKKLFFNLRRLKGEMQALEEGDLKPEQA
RLIAEKLNVTETEVYSMNGRMSGSDASLNVPMGTDGDMEWQDWLADDEPGTAETFANSQELDARMTLLTQAMEDLSERER
HILTERRLVDEPKTLEELSEQYNVSRERIRQIEVRAFEKLQKAMKRMAKEQGLPMGA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=282, Percent_Identity=40.0709219858156, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=35.2313167259786, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=32.9059829059829, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34147; Mature: 34015

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANANAKSSVALSPEQGMSRYLSEIRKFPMLEKNEEFMLARRWHEHEDTEAAEKMVTSHL
CCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
RLVAKIAMGYRGYGLPMAEVISEGNVGLMQAVKKFDPDKGFRLATYAMWWIRAAIQEYIL
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RSWSLVKLGTTAAQKKLFFNLRRLKGEMQALEEGDLKPEQARLIAEKLNVTETEVYSMNG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHEECCC
RMSGSDASLNVPMGTDGDMEWQDWLADDEPGTAETFANSQELDARMTLLTQAMEDLSERE
CCCCCCCEEECCCCCCCCCCHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RHILTERRLVDEPKTLEELSEQYNVSRERIRQIEVRAFEKLQKAMKRMAKEQGLPMGA
HHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANANAKSSVALSPEQGMSRYLSEIRKFPMLEKNEEFMLARRWHEHEDTEAAEKMVTSHL
CCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
RLVAKIAMGYRGYGLPMAEVISEGNVGLMQAVKKFDPDKGFRLATYAMWWIRAAIQEYIL
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RSWSLVKLGTTAAQKKLFFNLRRLKGEMQALEEGDLKPEQARLIAEKLNVTETEVYSMNG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHEECCC
RMSGSDASLNVPMGTDGDMEWQDWLADDEPGTAETFANSQELDARMTLLTQAMEDLSERE
CCCCCCCEEECCCCCCCCCCHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RHILTERRLVDEPKTLEELSEQYNVSRERIRQIEVRAFEKLQKAMKRMAKEQGLPMGA
HHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]