Click here to switch to the map view.

The map label for this gene is narI [H]

Identifier: 114797366

GI number: 114797366

Start: 1594775

End: 1595545

Strand: Direct

Name: narI [H]

Synonym: HNE_1564

Alternate gene names: 114797366

Gene position: 1594775-1595545 (Clockwise)

Preceding gene: 114797260

Following gene: 114799875

Centisome position: 43.04

GC content: 60.7

Gene sequence:

>771_bases
ATGGCTCAGGAACACGCAGTTACCCTTGTCAGCGCGGGCTGGTTCGACCAGGCCATGTTTGGCTACTACCCCTATGTCGC
GCTTGCCGTTCTCGCGATCGGATCGGTGCTTCGCTTCGACCGGGAACCCTATACATGGCGGTCTGGCTCCTCGCAGCTCC
TGCGGCGCAAACAGCTGATGCTCGGCTCCGTGCTTTTCCATGTCGGCGTGCTGGTGATCTTCGGAGGGCACTTCGTCGGC
CTTCTGACGCCTATCCAGGTCTTTGACGCGGTAGGTATCAGCCATGGTGCCAAGCAGGTGCTCGCCATCGTGGCCGGCGG
GATCGCCGGCGTCCTCGCTATTCTCGGAGCGTTGATCCTCCTTCATCGGCGCCTGTTCGATGCGCGGATCCGTGCGACTT
CGCGCTTTGGGGATACGGCGATCCTGGCGCTTCTGACTGCGCAGCTGGCGCTCGGCCTCTCAACGATTCCTTTGTCGGCG
CAGCACCTTGATGGATCGGAAATGGTGAAGTTCATGTCGTGGGCTCAGGGCATCTTCACATTCCGGCCGGGCGCTGCCAA
CTATGTCGCCGATGTCCACTGGATCTTCAAAGCACACCTGATCCTTGGGCTGACTATATTTCTTGTCTTTCCATTCACCC
GCCTAGTCCACATGTTGTCCGCGCCGGTCTGGTATATGGGGCGCCGTGGCTGGCAGCTGGTCCGCACACGCCGCGACATC
CATGCAGACAAGGCCAACGCAGCCGCAACAGCAGCACGGAGAGTCTCATGA

Upstream 100 bases:

>100_bases
AGCAGCATTCCGGGGTGCCGATACCGGTCCAGCAGATCGGACAAAATTCTGGCTGGCGCTGGAAACGCGCTTGTAGCCAA
CCAAACAGAGGAGGCCAGAC

Downstream 100 bases:

>100_bases
GCCCGCAGGCGGCCGTCTTCTTTGGCGTAGAAGTTCCTGAATCCCTCCTGGCGACAGAGCTTCAGCACCACCGGGCCCCT
ACGCTGTCGCAGGCGCGGGC

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAQEHAVTLVSAGWFDQAMFGYYPYVALAVLAIGSVLRFDREPYTWRSGSSQLLRRKQLMLGSVLFHVGVLVIFGGHFVG
LLTPIQVFDAVGISHGAKQVLAIVAGGIAGVLAILGALILLHRRLFDARIRATSRFGDTAILALLTAQLALGLSTIPLSA
QHLDGSEMVKFMSWAQGIFTFRPGAANYVADVHWIFKAHLILGLTIFLVFPFTRLVHMLSAPVWYMGRRGWQLVRTRRDI
HADKANAAATAARRVS

Sequences:

>Translated_256_residues
MAQEHAVTLVSAGWFDQAMFGYYPYVALAVLAIGSVLRFDREPYTWRSGSSQLLRRKQLMLGSVLFHVGVLVIFGGHFVG
LLTPIQVFDAVGISHGAKQVLAIVAGGIAGVLAILGALILLHRRLFDARIRATSRFGDTAILALLTAQLALGLSTIPLSA
QHLDGSEMVKFMSWAQGIFTFRPGAANYVADVHWIFKAHLILGLTIFLVFPFTRLVHMLSAPVWYMGRRGWQLVRTRRDI
HADKANAAATAARRVS
>Mature_255_residues
AQEHAVTLVSAGWFDQAMFGYYPYVALAVLAIGSVLRFDREPYTWRSGSSQLLRRKQLMLGSVLFHVGVLVIFGGHFVGL
LTPIQVFDAVGISHGAKQVLAIVAGGIAGVLAILGALILLHRRLFDARIRATSRFGDTAILALLTAQLALGLSTIPLSAQ
HLDGSEMVKFMSWAQGIFTFRPGAANYVADVHWIFKAHLILGLTIFLVFPFTRLVHMLSAPVWYMGRRGWQLVRTRRDIH
ADKANAAATAARRVS

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=222, Percent_Identity=52.2522522522522, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1787738, Length=216, Percent_Identity=51.3888888888889, Blast_Score=210, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 28080; Mature: 27948

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEHAVTLVSAGWFDQAMFGYYPYVALAVLAIGSVLRFDREPYTWRSGSSQLLRRKQLM
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LGSVLFHVGVLVIFGGHFVGLLTPIQVFDAVGISHGAKQVLAIVAGGIAGVLAILGALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHRRLFDARIRATSRFGDTAILALLTAQLALGLSTIPLSAQHLDGSEMVKFMSWAQGIFT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHCCCCHHHCCHHHHHHHHHHHHHHHE
FRPGAANYVADVHWIFKAHLILGLTIFLVFPFTRLVHMLSAPVWYMGRRGWQLVRTRRDI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
HADKANAAATAARRVS
HCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AQEHAVTLVSAGWFDQAMFGYYPYVALAVLAIGSVLRFDREPYTWRSGSSQLLRRKQLM
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LGSVLFHVGVLVIFGGHFVGLLTPIQVFDAVGISHGAKQVLAIVAGGIAGVLAILGALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHRRLFDARIRATSRFGDTAILALLTAQLALGLSTIPLSAQHLDGSEMVKFMSWAQGIFT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHCCCCHHHCCHHHHHHHHHHHHHHHE
FRPGAANYVADVHWIFKAHLILGLTIFLVFPFTRLVHMLSAPVWYMGRRGWQLVRTRRDI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
HADKANAAATAARRVS
HCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]