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The map label for this gene is nifM [H]

Identifier: 114799875

GI number: 114799875

Start: 1595542

End: 1596660

Strand: Direct

Name: nifM [H]

Synonym: HNE_1565

Alternate gene names: 114799875

Gene position: 1595542-1596660 (Clockwise)

Preceding gene: 114797366

Following gene: 114798885

Centisome position: 43.06

GC content: 62.47

Gene sequence:

>1119_bases
ATGAGCCCGCAGGCGGCCGTCTTCTTTGGCGTAGAAGTTCCTGAATCCCTCCTGGCGACAGAGCTTCAGCACCACCGGGC
CCCTACGCTGTCGCAGGCGCGGGCCCTTGCTGGGCGGGCGCTCGCAGCCAAAGCGGTCCTTCTGGCGCGGGGCCGGGAAC
TTGATCTCGTTGCTGAACCCGAGCGTAACTCCGAAGGTCAGGAGGAGACACCGGAGGAAGCGCTCATCCGCGCCGTGTTG
AATGAAGAGGTCGATGCGGATCCTGCTGCCGATGAGGCCGTGCGCGCAGTCTATGATGGCAATCCGGAGGGATTCAGGTC
GCCGCCGCTTTTGGAAGCGTCTCACATCCTCGTCCCTCCTGCGGATGCGAGCGAGGAAGCGTACCGTGCGGCGCGCGAGA
AGGCCCGAGCATTGATTGCTGACCTGCAGGCAAACCCGTCGGGATTTGCGAGGATGGCGGCAAGCAGGTCGGCCTGCCCG
TCGGCATCTGAAGGCGGCAGTCTCGGTCAGCTTCGTCCAGGAGATGTCCTGCCTCCTATCTGGACGGCGCTTACCAATAT
GGAAGAGGGGACAATAAGAGCCGAGCCGGTCGCAACCGAGCATGGCTGGCATGTGCTTCGCCTTGACCACCGATCCGATG
GGGAACGACTGCCGTTCGACTATGTGCGCCCGCACATCGCGCTCCAGCTTGAGGCGAGGGCGTGGACAAAATCGGCTGCC
TTGTATGTAGACCGGCTCCTTGCTGAGTCCGCCGCCAATCCCGGGCTCTGCCTGTCGGACAATGGGCGTCTGGACGACCG
CAACGGTATCGTCGCTCGGGCTGACGGATTGCTCGGCAAGGCACTGTCAGATGTGCAGATGGCATATGGTGCCCTCAGCG
AGGCTGCCCGTGGGCGCCTTGATGCTGCAGCCTCCAGAGAAAACGCAGCGCCAACTGAGTGCCTGAGCAGAGTGATCGGG
ACGTTCCTTTCAAAGGCCAATGACGAAGCCTGGACGCAGCTTATTTCCCGGTTGCGCGACAGCCAGGCGCCGTTCTCGGA
CTGCCTCGACCTGATCATATCCCATCAGCTGCCCTCGCAGCACGCAACACACACGCTCATCAGAATTAGAAACGGGTAG

Upstream 100 bases:

>100_bases
TCTGGTATATGGGGCGCCGTGGCTGGCAGCTGGTCCGCACACGCCGCGACATCCATGCAGACAAGGCCAACGCAGCCGCA
ACAGCAGCACGGAGAGTCTC

Downstream 100 bases:

>100_bases
CCGGCGGCCGCTGAAGAAAAGGATGCAGGTCATGGGTAGCACAGGGAAATTCCAGCCGATCGAACCGATCCCGGCCGACC
TTCTGCAGGAGCCGCTGATC

Product: putative peptidylprolyl cis-trans isomerase

Products: Isomerized Proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSPQAAVFFGVEVPESLLATELQHHRAPTLSQARALAGRALAAKAVLLARGRELDLVAEPERNSEGQEETPEEALIRAVL
NEEVDADPAADEAVRAVYDGNPEGFRSPPLLEASHILVPPADASEEAYRAAREKARALIADLQANPSGFARMAASRSACP
SASEGGSLGQLRPGDVLPPIWTALTNMEEGTIRAEPVATEHGWHVLRLDHRSDGERLPFDYVRPHIALQLEARAWTKSAA
LYVDRLLAESAANPGLCLSDNGRLDDRNGIVARADGLLGKALSDVQMAYGALSEAARGRLDAAASRENAAPTECLSRVIG
TFLSKANDEAWTQLISRLRDSQAPFSDCLDLIISHQLPSQHATHTLIRIRNG

Sequences:

>Translated_372_residues
MSPQAAVFFGVEVPESLLATELQHHRAPTLSQARALAGRALAAKAVLLARGRELDLVAEPERNSEGQEETPEEALIRAVL
NEEVDADPAADEAVRAVYDGNPEGFRSPPLLEASHILVPPADASEEAYRAAREKARALIADLQANPSGFARMAASRSACP
SASEGGSLGQLRPGDVLPPIWTALTNMEEGTIRAEPVATEHGWHVLRLDHRSDGERLPFDYVRPHIALQLEARAWTKSAA
LYVDRLLAESAANPGLCLSDNGRLDDRNGIVARADGLLGKALSDVQMAYGALSEAARGRLDAAASRENAAPTECLSRVIG
TFLSKANDEAWTQLISRLRDSQAPFSDCLDLIISHQLPSQHATHTLIRIRNG
>Mature_371_residues
SPQAAVFFGVEVPESLLATELQHHRAPTLSQARALAGRALAAKAVLLARGRELDLVAEPERNSEGQEETPEEALIRAVLN
EEVDADPAADEAVRAVYDGNPEGFRSPPLLEASHILVPPADASEEAYRAAREKARALIADLQANPSGFARMAASRSACPS
ASEGGSLGQLRPGDVLPPIWTALTNMEEGTIRAEPVATEHGWHVLRLDHRSDGERLPFDYVRPHIALQLEARAWTKSAAL
YVDRLLAESAANPGLCLSDNGRLDDRNGIVARADGLLGKALSDVQMAYGALSEAARGRLDAAASRENAAPTECLSRVIGT
FLSKANDEAWTQLISRLRDSQAPFSDCLDLIISHQLPSQHATHTLIRIRNG

Specific function: Required for the activation and stabilization of the iron-component (nifH) of nitrogenase. Probable PPIase [H]

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014282
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: 5.2.1.8 [C]

Molecular weight: Translated: 40006; Mature: 39875

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQAAVFFGVEVPESLLATELQHHRAPTLSQARALAGRALAAKAVLLARGRELDLVAEP
CCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
ERNSEGQEETPEEALIRAVLNEEVDADPAADEAVRAVYDGNPEGFRSPPLLEASHILVPP
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
ADASEEAYRAAREKARALIADLQANPSGFARMAASRSACPSASEGGSLGQLRPGDVLPPI
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
WTALTNMEEGTIRAEPVATEHGWHVLRLDHRSDGERLPFDYVRPHIALQLEARAWTKSAA
HHHHHCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEEHHHHHHHHH
LYVDRLLAESAANPGLCLSDNGRLDDRNGIVARADGLLGKALSDVQMAYGALSEAARGRL
HHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCH
DAAASRENAAPTECLSRVIGTFLSKANDEAWTQLISRLRDSQAPFSDCLDLIISHQLPSQ
HHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCH
HATHTLIRIRNG
HHHEEEEEEECC
>Mature Secondary Structure 
SPQAAVFFGVEVPESLLATELQHHRAPTLSQARALAGRALAAKAVLLARGRELDLVAEP
CCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
ERNSEGQEETPEEALIRAVLNEEVDADPAADEAVRAVYDGNPEGFRSPPLLEASHILVPP
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
ADASEEAYRAAREKARALIADLQANPSGFARMAASRSACPSASEGGSLGQLRPGDVLPPI
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
WTALTNMEEGTIRAEPVATEHGWHVLRLDHRSDGERLPFDYVRPHIALQLEARAWTKSAA
HHHHHCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEEHHHHHHHHH
LYVDRLLAESAANPGLCLSDNGRLDDRNGIVARADGLLGKALSDVQMAYGALSEAARGRL
HHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCH
DAAASRENAAPTECLSRVIGTFLSKANDEAWTQLISRLRDSQAPFSDCLDLIISHQLPSQ
HHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCH
HATHTLIRIRNG
HHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proline Imidic Peptide Bonds In Oligopeptides [C]

Specific reaction: Cis-Trans Isomerization Of Proline Imidic Peptide Bonds In Oligopeptides. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885524 [H]