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The map label for this gene is grpE

Identifier: 114797336

GI number: 114797336

Start: 3472065

End: 3472631

Strand: Direct

Name: grpE

Synonym: HNE_3318

Alternate gene names: 114797336

Gene position: 3472065-3472631 (Clockwise)

Preceding gene: 114800305

Following gene: 114798319

Centisome position: 93.71

GC content: 66.14

Gene sequence:

>567_bases
ATGAGCGACGAAAACAAACCGGGTGAGGCCGCTGAACTCGACGCCGGCGTAGCCCCTGAGGCCCAGCCCGAGACGGAACT
GACCGTTGAGGAGCTGATTATCAGGCTGGAAGCGGAAAAAGCCGACATGAACGGCCAGATCCTGCGCCTGCTGGCTGACC
TCGACAATACCCGCAAGCGCGCCGACCGTCAGGTCAGCGAGGCCCGCATCTATGCCATCGAAAAGTTCGCCGCAGACCTG
CTCAGCGTCTCCGACAACCTCTCCCGCGCCCTCGACGCCCTGCCCGATAGCGAGCGTGAAAACCTCACCGATGCCGGCAA
AAACCTCCTCGGCGGCATCGAGATGACCGCCAAGGAACTCAACACCGCCCTCTCCCGCCACGGCGTCGTACCCGTACCGG
CCGAGCCCGGCGCGGTGTTTGATCCCAACGTCCATCAGGCCGTCGCCCAGATCCCGTCCCCCCAGCCCAGCGGCACCATC
GCCCAGCTGTTCCAGCCCGGCTGGAAGATCGGGGACCGCACCCTGCGCGCCGCCATGGTCGCCGTCAGCACCGGCCCGGC
TAACTGA

Upstream 100 bases:

>100_bases
CAACCCGGCTCAACTACGCCAGGGTCATTCCGATGGTCGATTATACCGCCAAGGTCGTGGGGGACATCCTCTCACACCGG
CGCGGAAAGGATGGGAAGTG

Downstream 100 bases:

>100_bases
TCTGGCAAAGGTGCGGGCTCAGTCCCGCACCACCCGCCATGCCGTGACGGTGCCCGGCACCCAGTTGGGCACGATCACCA
CATCGCCAAACACCGACAGG

Product: co-chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSDENKPGEAAELDAGVAPEAQPETELTVEELIIRLEAEKADMNGQILRLLADLDNTRKRADRQVSEARIYAIEKFAADL
LSVSDNLSRALDALPDSERENLTDAGKNLLGGIEMTAKELNTALSRHGVVPVPAEPGAVFDPNVHQAVAQIPSPQPSGTI
AQLFQPGWKIGDRTLRAAMVAVSTGPAN

Sequences:

>Translated_188_residues
MSDENKPGEAAELDAGVAPEAQPETELTVEELIIRLEAEKADMNGQILRLLADLDNTRKRADRQVSEARIYAIEKFAADL
LSVSDNLSRALDALPDSERENLTDAGKNLLGGIEMTAKELNTALSRHGVVPVPAEPGAVFDPNVHQAVAQIPSPQPSGTI
AQLFQPGWKIGDRTLRAAMVAVSTGPAN
>Mature_187_residues
SDENKPGEAAELDAGVAPEAQPETELTVEELIIRLEAEKADMNGQILRLLADLDNTRKRADRQVSEARIYAIEKFAADLL
SVSDNLSRALDALPDSERENLTDAGKNLLGGIEMTAKELNTALSRHGVVPVPAEPGAVFDPNVHQAVAQIPSPQPSGTIA
QLFQPGWKIGDRTLRAAMVAVSTGPAN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=164, Percent_Identity=35.9756097560976, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI40255109, Length=158, Percent_Identity=30.379746835443, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788967, Length=177, Percent_Identity=35.0282485875706, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=36.6666666666667, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=190, Percent_Identity=30, Blast_Score=88, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24653432, Length=185, Percent_Identity=36.2162162162162, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_HYPNA (Q0BX02)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761991.1
- ProteinModelPortal:   Q0BX02
- SMR:   Q0BX02
- STRING:   Q0BX02
- GeneID:   4289414
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3318
- NMPDR:   fig|228405.5.peg.3178
- TIGR:   HNE_3318
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   LEHNVEG
- PhylomeDB:   Q0BX02
- ProtClustDB:   CLSK2531648
- BioCyc:   HNEP81032:HNE_3318-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20085; Mature: 19954

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDENKPGEAAELDAGVAPEAQPETELTVEELIIRLEAEKADMNGQILRLLADLDNTRKR
CCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ADRQVSEARIYAIEKFAADLLSVSDNLSRALDALPDSERENLTDAGKNLLGGIEMTAKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
NTALSRHGVVPVPAEPGAVFDPNVHQAVAQIPSPQPSGTIAQLFQPGWKIGDRTLRAAMV
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
AVSTGPAN
HHCCCCCC
>Mature Secondary Structure 
SDENKPGEAAELDAGVAPEAQPETELTVEELIIRLEAEKADMNGQILRLLADLDNTRKR
CCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ADRQVSEARIYAIEKFAADLLSVSDNLSRALDALPDSERENLTDAGKNLLGGIEMTAKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
NTALSRHGVVPVPAEPGAVFDPNVHQAVAQIPSPQPSGTIAQLFQPGWKIGDRTLRAAMV
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
AVSTGPAN
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA