Click here to switch to the map view.

The map label for this gene is oprF [H]

Identifier: 114797292

GI number: 114797292

Start: 1462127

End: 1463761

Strand: Reverse

Name: oprF [H]

Synonym: HNE_1432

Alternate gene names: 114797292

Gene position: 1463761-1462127 (Counterclockwise)

Preceding gene: 114799883

Following gene: 114798358

Centisome position: 39.51

GC content: 65.26

Gene sequence:

>1635_bases
ATGTCTCATGTGCAGACGCTGCTGGCCGCCGCCAGCCTTATCCTGCTGGCTGCCTGTGGCGGCAGCGAAACGGCGCCCGC
CACTCCCGAAGCGCCTGAGGCGGCGCCGGCTGCCGATGCGGAAAACGAGGCGCAAGTGACGCCTGCCGCCGATCCGGGTT
TCGTCCGCCCCGAAGGCCTGCCGGAAACGGCAATCCCGGTGACCGATCTGGATGGCTCGGTCTATTGGGTCGAGCCGCGC
GGCGACGGGCGCGTGGCGCTCTACTCCTTCACCGATGATGGACAGGTGGGCGCCGCTGCGGGCACCTTCGCCCAGCTGCA
GGAAGCTTATCCCACCCTCGATCTCAGCCCGCTGGCCGACCGGTTGGACCTCGCCGGGGAAGCCGCTGCGGACCAAGAAA
CAGGCTCTGGAGTGGACACCCTGCGCGCAGCGCAGGACGGGCTGGTGGCGGCCACGCCCGGCGCCCAGAAGGCCCTCGAC
CTTGCGCGCAATGGCCCGGTCCTGGAGCTGACCGGCCCGACCCTGCACCATCTTGTCGAAAGCATGGGTGGCTCGTTCAC
AATGACGAAGGAAGAGCTGACCGCCCTGCTGCAGGAACAGACGGACGGCCGCTTCACAGCGACCAATGCCGAGCTCAAAG
CGTTGATCAGGGGAAGCGGCGAGGACGGCATCACGGCCGAGGACATAGGCCTGCTCCTGCCCAAGACCCGGGACGCGCCG
CCGCCGAAGATCGACCGCGACGCGCCCGGCAAGGGCCGCGCGGGCGACGACTGCCCGGGCCTCATCTATGGCAGTGGCAA
GCGCGAGATCTGCCTGCCGCTCGGCGCGCTTTCCTTTGCCGACAGGGTGGTGAGCTTCACGCCCGGCGAGAAGCCCTCCA
AGCCACCCTTCGACTTTCCGGAGAACGCCCTTGGCGAGCCGAACTATCGCAACACCTACAGCGCCGACTTCATCTCGCTC
GGCTGCAATGGCGAACTGATTACGCAGTTCACGGACAATATCCTGGTCGATGTCGACGGGGTGGATCTCTACATTTTCGA
GATCGGGCCCGTGGTGGAGAAAACCCTGCTCGCCATTTCCTCTGACGGGGAAAGCTGGATTGAGGTGGGCGAGATCGAAG
GCGCCCGGTCGGAGGTCGATATCGGGCCCTTCGTGAGCAAGGGCGACAAGTTTCCCTTTGTCCGGCTGACCAATGCCAGC
GTTGCCTGCGGGGGCAATCATTCAGGCGCCGATATTGATGCCATCGCGGCCGTGGGCGCAGAAATCCGCCTGTCGCTCGA
CAGCGCCCTGCTGTTCGATGTCGGCAAGTATGAGATCAAGCCTGAGGCGGAAAGCGCCCTGGCAGAGCTTGCCGCGCAGC
TGCAGACTTATGGCGCTGACATCCGCGTAACGGTCGAGGGTCATACGGACTCCACCGGCTCGGCGGCGTCCAACCAGACA
CTGTCTGAAAACCGCGCACGCTCCGTCTGGGACAATCTCTCGGGCAAGATTTCGCCTGTGCCGGAGGACGTGACGATCAG
GGGCTATGGCGCAAGCCGTCCGGTGGCGGACAATGCGACCGAAGAAGGCCGCGCGGAAAACCGCCGCGTCGATATTCTCA
TCCTGCCCGGCAAGAAGGGTTATCTGACGGAGTGA

Upstream 100 bases:

>100_bases
GCCGCTGCGAGGCCGAACACAACAACAATCCCATGTGGCGCTCCACCGAGGGCGAGATGTTCTACAGGACGATCAGGGCC
AGTTATCAGAGGACGAACTG

Downstream 100 bases:

>100_bases
TCCCATGAAAGCGCTGAAAATCCTTCCCCTCCTCGCCCTTCTGGCGGCCGCCACAGCCTGCGGCGGCAGTGAACCGGCCG
CGCCCGTGGAGGCGCCCCTC

Product: OmpA family membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSHVQTLLAAASLILLAACGGSETAPATPEAPEAAPAADAENEAQVTPAADPGFVRPEGLPETAIPVTDLDGSVYWVEPR
GDGRVALYSFTDDGQVGAAAGTFAQLQEAYPTLDLSPLADRLDLAGEAAADQETGSGVDTLRAAQDGLVAATPGAQKALD
LARNGPVLELTGPTLHHLVESMGGSFTMTKEELTALLQEQTDGRFTATNAELKALIRGSGEDGITAEDIGLLLPKTRDAP
PPKIDRDAPGKGRAGDDCPGLIYGSGKREICLPLGALSFADRVVSFTPGEKPSKPPFDFPENALGEPNYRNTYSADFISL
GCNGELITQFTDNILVDVDGVDLYIFEIGPVVEKTLLAISSDGESWIEVGEIEGARSEVDIGPFVSKGDKFPFVRLTNAS
VACGGNHSGADIDAIAAVGAEIRLSLDSALLFDVGKYEIKPEAESALAELAAQLQTYGADIRVTVEGHTDSTGSAASNQT
LSENRARSVWDNLSGKISPVPEDVTIRGYGASRPVADNATEEGRAENRRVDILILPGKKGYLTE

Sequences:

>Translated_544_residues
MSHVQTLLAAASLILLAACGGSETAPATPEAPEAAPAADAENEAQVTPAADPGFVRPEGLPETAIPVTDLDGSVYWVEPR
GDGRVALYSFTDDGQVGAAAGTFAQLQEAYPTLDLSPLADRLDLAGEAAADQETGSGVDTLRAAQDGLVAATPGAQKALD
LARNGPVLELTGPTLHHLVESMGGSFTMTKEELTALLQEQTDGRFTATNAELKALIRGSGEDGITAEDIGLLLPKTRDAP
PPKIDRDAPGKGRAGDDCPGLIYGSGKREICLPLGALSFADRVVSFTPGEKPSKPPFDFPENALGEPNYRNTYSADFISL
GCNGELITQFTDNILVDVDGVDLYIFEIGPVVEKTLLAISSDGESWIEVGEIEGARSEVDIGPFVSKGDKFPFVRLTNAS
VACGGNHSGADIDAIAAVGAEIRLSLDSALLFDVGKYEIKPEAESALAELAAQLQTYGADIRVTVEGHTDSTGSAASNQT
LSENRARSVWDNLSGKISPVPEDVTIRGYGASRPVADNATEEGRAENRRVDILILPGKKGYLTE
>Mature_543_residues
SHVQTLLAAASLILLAACGGSETAPATPEAPEAAPAADAENEAQVTPAADPGFVRPEGLPETAIPVTDLDGSVYWVEPRG
DGRVALYSFTDDGQVGAAAGTFAQLQEAYPTLDLSPLADRLDLAGEAAADQETGSGVDTLRAAQDGLVAATPGAQKALDL
ARNGPVLELTGPTLHHLVESMGGSFTMTKEELTALLQEQTDGRFTATNAELKALIRGSGEDGITAEDIGLLLPKTRDAPP
PKIDRDAPGKGRAGDDCPGLIYGSGKREICLPLGALSFADRVVSFTPGEKPSKPPFDFPENALGEPNYRNTYSADFISLG
CNGELITQFTDNILVDVDGVDLYIFEIGPVVEKTLLAISSDGESWIEVGEIEGARSEVDIGPFVSKGDKFPFVRLTNASV
ACGGNHSGADIDAIAAVGAEIRLSLDSALLFDVGKYEIKPEAESALAELAAQLQTYGADIRVTVEGHTDSTGSAASNQTL
SENRARSVWDNLSGKISPVPEDVTIRGYGASRPVADNATEEGRAENRRVDILILPGKKGYLTE

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=113, Percent_Identity=37.1681415929204, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787191, Length=112, Percent_Identity=39.2857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994946, Length=120, Percent_Identity=37.5, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 56888; Mature: 56756

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVQTLLAAASLILLAACGGSETAPATPEAPEAAPAADAENEAQVTPAADPGFVRPEGL
CHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCC
PETAIPVTDLDGSVYWVEPRGDGRVALYSFTDDGQVGAAAGTFAQLQEAYPTLDLSPLAD
CCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
RLDLAGEAAADQETGSGVDTLRAAQDGLVAATPGAQKALDLARNGPVLELTGPTLHHLVE
HHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEECCCHHHHHHH
SMGGSFTMTKEELTALLQEQTDGRFTATNAELKALIRGSGEDGITAEDIGLLLPKTRDAP
HCCCCEEECHHHHHHHHHHCCCCCEEECCHHHHEEEECCCCCCCCHHHHEEECCCCCCCC
PPKIDRDAPGKGRAGDDCPGLIYGSGKREICLPLGALSFADRVVSFTPGEKPSKPPFDFP
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECHHHHHHHHEEECCCCCCCCCCCCCC
ENALGEPNYRNTYSADFISLGCNGELITQFTDNILVDVDGVDLYIFEIGPVVEKTLLAIS
CCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEEECCCEEEEEECCHHHHHHEEEEE
SDGESWIEVGEIEGARSEVDIGPFVSKGDKFPFVRLTNASVACGGNHSGADIDAIAAVGA
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHCCC
EIRLSLDSALLFDVGKYEIKPEAESALAELAAQLQTYGADIRVTVEGHTDSTGSAASNQT
EEEEEECCEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
LSENRARSVWDNLSGKISPVPEDVTIRGYGASRPVADNATEEGRAENRRVDILILPGKKG
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
YLTE
CCCC
>Mature Secondary Structure 
SHVQTLLAAASLILLAACGGSETAPATPEAPEAAPAADAENEAQVTPAADPGFVRPEGL
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCC
PETAIPVTDLDGSVYWVEPRGDGRVALYSFTDDGQVGAAAGTFAQLQEAYPTLDLSPLAD
CCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
RLDLAGEAAADQETGSGVDTLRAAQDGLVAATPGAQKALDLARNGPVLELTGPTLHHLVE
HHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEECCCHHHHHHH
SMGGSFTMTKEELTALLQEQTDGRFTATNAELKALIRGSGEDGITAEDIGLLLPKTRDAP
HCCCCEEECHHHHHHHHHHCCCCCEEECCHHHHEEEECCCCCCCCHHHHEEECCCCCCCC
PPKIDRDAPGKGRAGDDCPGLIYGSGKREICLPLGALSFADRVVSFTPGEKPSKPPFDFP
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECHHHHHHHHEEECCCCCCCCCCCCCC
ENALGEPNYRNTYSADFISLGCNGELITQFTDNILVDVDGVDLYIFEIGPVVEKTLLAIS
CCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEEECCCEEEEEECCHHHHHHEEEEE
SDGESWIEVGEIEGARSEVDIGPFVSKGDKFPFVRLTNASVACGGNHSGADIDAIAAVGA
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHCCC
EIRLSLDSALLFDVGKYEIKPEAESALAELAAQLQTYGADIRVTVEGHTDSTGSAASNQT
EEEEEECCEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
LSENRARSVWDNLSGKISPVPEDVTIRGYGASRPVADNATEEGRAENRRVDILILPGKKG
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
YLTE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]