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The map label for this gene is hemC

Identifier: 114797250

GI number: 114797250

Start: 3395793

End: 3396758

Strand: Direct

Name: hemC

Synonym: HNE_3227

Alternate gene names: 114797250

Gene position: 3395793-3396758 (Clockwise)

Preceding gene: 114797603

Following gene: 114800364

Centisome position: 91.65

GC content: 66.15

Gene sequence:

>966_bases
ATGACCCAGATAGACCCTCCCAAAGGCGGCAAACGCCTCCGGCTTGGAACGCGCGGCTCTCCGCTGGCGCTGGCCCAGGC
CCACCAGATCGCAGACGGTATCCGCGCCGCTTCGGCCGGCGCCTATGACTGCGAAATCGTGGCTTTCACGACCACCGGCG
ACAAGCTGACCACCGAACGGCTGATCAACTCCGGCGGCAAGGGCCTGTTCACGCGCGAGCTGGACGATGCCCTTTCGCGC
GGAGAGCTTGATCTGGCCGTCCACAGTCTCAAGGACGTCCCCTCGGTGCTGCCACCTGGTCAGATCTTCGCTGCGTTCCC
GAAGCGGGAAGATCCCCGCGACGGCTTCCTCTCCCACGGCGCGAAGTCGATCCAGGACCTCCCCGAAGGTGCCACACTCG
GCACCGCCTCCCTGCGCCGCGAAGCTCAAGCGCTCGCCCTGCGGCCAGACCTCAAAGTTGTCACCTTCCGTGGCAATGTC
GCCACCCGCATGCGCAAGCTCGAAGAGGGCCTGGCTGACGCCACCTTCCTGGCGATGGCCGGCCTCACCCGCCTTGGCCT
TTCAGAGGTTGCCACCCCCATCCCGCTCGAAGACATGCTGCCCGCTGCCGGGCAAGGCATCATCGGCGTGGTCACACGTG
ATGATGTGGATACGGAGCTTCTGGAGATTCTCGGCCAGCTCAGCCATGAGCCGAGCCGCGTCGCCGCGATTGCCGAGCGC
GCCTTCCTCGAAAAGCTCGATGGCAGCTGCCGCACGCCGATTGCCGCCCACCTGTTTGACCGAGGCGATGAATGGCAGCT
GATCGGGGAAGTGCTCAGCCCCGGCGGTACGACCCGCTGGCGCGGAGACGGGGTATGCCGCAAGGATGCCACCGATGCCC
AGCTGGTCCTCTTGGGCCGGGCCGTCGCGCAGGAGATCATCTTCGCGGCCAAGGAAGCCCTTCCCGTTTTCGGAGACGAG
CGTTGA

Upstream 100 bases:

>100_bases
CGAATCCTCCAAGGTATTTGCCGTAAAACGATAAGGATGGGTACCCCCCTGCGCTCCCGGCGTCAATTGGCAAGCGCCGC
TTCCGCGGTTAGGGAGAACC

Downstream 100 bases:

>100_bases
ATCTGGCGGTGATCGTCACGCGCACACAGCCGGGCGCGGACGAAACCGCCGCCCGTCTCACCGCGCTGGGCTACCACGCC
ATCCTCTCGCCCATGCTGTC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTQIDPPKGGKRLRLGTRGSPLALAQAHQIADGIRAASAGAYDCEIVAFTTTGDKLTTERLINSGGKGLFTRELDDALSR
GELDLAVHSLKDVPSVLPPGQIFAAFPKREDPRDGFLSHGAKSIQDLPEGATLGTASLRREAQALALRPDLKVVTFRGNV
ATRMRKLEEGLADATFLAMAGLTRLGLSEVATPIPLEDMLPAAGQGIIGVVTRDDVDTELLEILGQLSHEPSRVAAIAER
AFLEKLDGSCRTPIAAHLFDRGDEWQLIGEVLSPGGTTRWRGDGVCRKDATDAQLVLLGRAVAQEIIFAAKEALPVFGDE
R

Sequences:

>Translated_321_residues
MTQIDPPKGGKRLRLGTRGSPLALAQAHQIADGIRAASAGAYDCEIVAFTTTGDKLTTERLINSGGKGLFTRELDDALSR
GELDLAVHSLKDVPSVLPPGQIFAAFPKREDPRDGFLSHGAKSIQDLPEGATLGTASLRREAQALALRPDLKVVTFRGNV
ATRMRKLEEGLADATFLAMAGLTRLGLSEVATPIPLEDMLPAAGQGIIGVVTRDDVDTELLEILGQLSHEPSRVAAIAER
AFLEKLDGSCRTPIAAHLFDRGDEWQLIGEVLSPGGTTRWRGDGVCRKDATDAQLVLLGRAVAQEIIFAAKEALPVFGDE
R
>Mature_320_residues
TQIDPPKGGKRLRLGTRGSPLALAQAHQIADGIRAASAGAYDCEIVAFTTTGDKLTTERLINSGGKGLFTRELDDALSRG
ELDLAVHSLKDVPSVLPPGQIFAAFPKREDPRDGFLSHGAKSIQDLPEGATLGTASLRREAQALALRPDLKVVTFRGNVA
TRMRKLEEGLADATFLAMAGLTRLGLSEVATPIPLEDMLPAAGQGIIGVVTRDDVDTELLEILGQLSHEPSRVAAIAERA
FLEKLDGSCRTPIAAHLFDRGDEWQLIGEVLSPGGTTRWRGDGVCRKDATDAQLVLLGRAVAQEIIFAAKEALPVFGDER

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=270, Percent_Identity=41.4814814814815, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI66933009, Length=270, Percent_Identity=41.4814814814815, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI48994974, Length=274, Percent_Identity=44.1605839416058, Blast_Score=199, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319996, Length=258, Percent_Identity=36.046511627907, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20130425, Length=253, Percent_Identity=38.7351778656126, Blast_Score=150, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_HYPNA (Q0BX92)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761901.1
- ProteinModelPortal:   Q0BX92
- SMR:   Q0BX92
- STRING:   Q0BX92
- GeneID:   4288448
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3227
- NMPDR:   fig|228405.5.peg.3090
- TIGR:   HNE_3227
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- PhylomeDB:   Q0BX92
- BioCyc:   HNEP81032:HNE_3227-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34258; Mature: 34126

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIDPPKGGKRLRLGTRGSPLALAQAHQIADGIRAASAGAYDCEIVAFTTTGDKLTTER
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHH
LINSGGKGLFTRELDDALSRGELDLAVHSLKDVPSVLPPGQIFAAFPKREDPRDGFLSHG
HHHCCCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHC
AKSIQDLPEGATLGTASLRREAQALALRPDLKVVTFRGNVATRMRKLEEGLADATFLAMA
HHHHHHCCCCCCCCHHHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
GLTRLGLSEVATPIPLEDMLPAAGQGIIGVVTRDDVDTELLEILGQLSHEPSRVAAIAER
HHHHHCHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHH
AFLEKLDGSCRTPIAAHLFDRGDEWQLIGEVLSPGGTTRWRGDGVCRKDATDAQLVLLGR
HHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AVAQEIIFAAKEALPVFGDER
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQIDPPKGGKRLRLGTRGSPLALAQAHQIADGIRAASAGAYDCEIVAFTTTGDKLTTER
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHH
LINSGGKGLFTRELDDALSRGELDLAVHSLKDVPSVLPPGQIFAAFPKREDPRDGFLSHG
HHHCCCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHC
AKSIQDLPEGATLGTASLRREAQALALRPDLKVVTFRGNVATRMRKLEEGLADATFLAMA
HHHHHHCCCCCCCCHHHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
GLTRLGLSEVATPIPLEDMLPAAGQGIIGVVTRDDVDTELLEILGQLSHEPSRVAAIAER
HHHHHCHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHH
AFLEKLDGSCRTPIAAHLFDRGDEWQLIGEVLSPGGTTRWRGDGVCRKDATDAQLVLLGR
HHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AVAQEIIFAAKEALPVFGDER
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA