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The map label for this gene is hisA

Identifier: 114797166

GI number: 114797166

Start: 56214

End: 56939

Strand: Reverse

Name: hisA

Synonym: HNE_0070

Alternate gene names: 114797166

Gene position: 56939-56214 (Counterclockwise)

Preceding gene: 114798251

Following gene: 114797557

Centisome position: 1.54

GC content: 67.77

Gene sequence:

>726_bases
ATGACCTTCACCCTCTATCCCGCCATTGACCTGAAAGACGGCGCCTGCGTGCGCCTCCTGCGCGGGGAGATGGACGCGGC
CACCGTGTTCAACACGGACCCCGCCGACCAGGCCCGCGCCTTTCATGCGATGGGCTTCACCCATCTGCATGTGGTGGACC
TCAACGGGGCGTTCGCCGGCGCGCCGGTCAACCGCGCGGCCGTCGAAGGCATCCTGAAGGCCACCCCTGCCCCGGTACAG
CTGGGCGGTGGCATCCGCACGCGGGCGCAGATTGATGCCTGGCTGGAAGCGGGCATCAGCCGCGTCATCCTCGGCACCAT
TGCCCTGCGCGACCCCGAACTGGTGAAAACAGCTGCCCGCGCGCTGCCCGGCCGCATCGTGGTGGGGATCGACGCGAAGG
ATGGCATGGTGGCCGTGGAAGGCTGGGCCGAGACCAGCGACATGAAGGCGACCGAGCTTGCCAAGGCGTTTGAAGGCTGC
GGCGTGGCCGCCATCGTCGCCACCGACATTGGCCGCGACGGACTGAAAACCGGCGTCAATGTTCCGTTCACGGCAGAGCT
CGCCCACGCCGTCTCCATCCCCGTCATCGCCTCCGGCGGCGTGCGCGACGTGAACGACATCCGTGCCCTGAAGGCTTCGG
GCGCACCGATTGCGGGGTCTATCCTGGGGCGGGCGCTGTATGATGGGGATATTGTGGCGAGCGAGGCGATTGAGGCGGCG
GGTTAG

Upstream 100 bases:

>100_bases
CCGCCATCGCGCGCGACAACCTCATCGGCACGCAATTCCATCCCGAAAAGAGCCAGCGCCTCGGCCTGACGCTGCTCGCC
AACTTCCTGAAATGGCACCC

Downstream 100 bases:

>100_bases
GCTAAGGAACCTAACCCGCCGGTGGCTCATGTCGTGTGACACGCCAGCGAGCAGTAATAACACCCGTTGGACTGCCAATA
TGATTCTTTAGCCTTTCGCA

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTFTLYPAIDLKDGACVRLLRGEMDAATVFNTDPADQARAFHAMGFTHLHVVDLNGAFAGAPVNRAAVEGILKATPAPVQ
LGGGIRTRAQIDAWLEAGISRVILGTIALRDPELVKTAARALPGRIVVGIDAKDGMVAVEGWAETSDMKATELAKAFEGC
GVAAIVATDIGRDGLKTGVNVPFTAELAHAVSIPVIASGGVRDVNDIRALKASGAPIAGSILGRALYDGDIVASEAIEAA
G

Sequences:

>Translated_241_residues
MTFTLYPAIDLKDGACVRLLRGEMDAATVFNTDPADQARAFHAMGFTHLHVVDLNGAFAGAPVNRAAVEGILKATPAPVQ
LGGGIRTRAQIDAWLEAGISRVILGTIALRDPELVKTAARALPGRIVVGIDAKDGMVAVEGWAETSDMKATELAKAFEGC
GVAAIVATDIGRDGLKTGVNVPFTAELAHAVSIPVIASGGVRDVNDIRALKASGAPIAGSILGRALYDGDIVASEAIEAA
G
>Mature_240_residues
TFTLYPAIDLKDGACVRLLRGEMDAATVFNTDPADQARAFHAMGFTHLHVVDLNGAFAGAPVNRAAVEGILKATPAPVQL
GGGIRTRAQIDAWLEAGISRVILGTIALRDPELVKTAARALPGRIVVGIDAKDGMVAVEGWAETSDMKATELAKAFEGCG
VAAIVATDIGRDGLKTGVNVPFTAELAHAVSIPVIASGGVRDVNDIRALKASGAPIAGSILGRALYDGDIVASEAIEAAG

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=35, Blast_Score=131, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_HYPNA (Q0C639)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758804.1
- ProteinModelPortal:   Q0C639
- SMR:   Q0C639
- STRING:   Q0C639
- GeneID:   4287625
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0070
- NMPDR:   fig|228405.5.peg.69
- TIGR:   HNE_0070
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q0C639
- ProtClustDB:   CLSK2531615
- BioCyc:   HNEP81032:HNE_0070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 24734; Mature: 24602

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTLYPAIDLKDGACVRLLRGEMDAATVFNTDPADQARAFHAMGFTHLHVVDLNGAFAG
CEEEEEEEEECCCCCEEHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCC
APVNRAAVEGILKATPAPVQLGGGIRTRAQIDAWLEAGISRVILGTIALRDPELVKTAAR
CCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHH
ALPGRIVVGIDAKDGMVAVEGWAETSDMKATELAKAFEGCGVAAIVATDIGRDGLKTGVN
HCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCC
VPFTAELAHAVSIPVIASGGVRDVNDIRALKASGAPIAGSILGRALYDGDIVASEAIEAA
CCCHHHHHHHEECCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
G
C
>Mature Secondary Structure 
TFTLYPAIDLKDGACVRLLRGEMDAATVFNTDPADQARAFHAMGFTHLHVVDLNGAFAG
EEEEEEEEECCCCCEEHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCC
APVNRAAVEGILKATPAPVQLGGGIRTRAQIDAWLEAGISRVILGTIALRDPELVKTAAR
CCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHH
ALPGRIVVGIDAKDGMVAVEGWAETSDMKATELAKAFEGCGVAAIVATDIGRDGLKTGVN
HCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCC
VPFTAELAHAVSIPVIASGGVRDVNDIRALKASGAPIAGSILGRALYDGDIVASEAIEAA
CCCHHHHHHHEECCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA