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The map label for this gene is hisF

Identifier: 114797557

GI number: 114797557

Start: 53725

End: 54483

Strand: Reverse

Name: hisF

Synonym: HNE_0066

Alternate gene names: 114797557

Gene position: 54483-53725 (Counterclockwise)

Preceding gene: 114797166

Following gene: 114797533

Centisome position: 1.47

GC content: 67.98

Gene sequence:

>759_bases
ATGCTGAAAACCCGCATCATCCCCTGCCTCGACGTGAAAGACGGGCGCACCGTGAAGGGCGTCAATTTCGTGGACCTGAA
AGACGCCGGAGACCCCGTCGCCCTTGCCCGCGCCTATGACGCGGCGGGCGCCGACGAGCTTTGCTTCCTCGACATCACCG
CCAGCCATGAAGGCCGCGGCACGCTGCTGGAAACGGTCAGCCGCACGGCTGAGGCGTGCTTCATGCCGCTGACCGTTGGC
GGGGGCGTGCGCTCGGTGGAAGATATGGTGGCGCTGTTGCGCGCAGGGGCGGACAAGGTGGCCATCAATTCGGCCGCCGT
GGCAGACCCGGCGCTGGTCTCCGCCTGCGCGGCGAAGGCTGGGCGGCAATGCGTGGTGGTGGCCATTGATGCGCGCGCCG
TCGGCGACAGCTGGGAAATCTTCACCCATGGCGGGCGCAAGGCCACGGGCATCAACGCAGTCGAGTTTGCCCGCGAGGCC
GCCAAAAGGGGCGCGGGCGAAATCCTGCTTACCTCAATGGACCGGGACGGGACGAAATCTGGCTATGACATTCCGCTGCT
GAAGGCGGTCTCGTCTGCCGTCACCATCCCCGTCATCGCCAGTGGCGGGGCCGGGAGCGTCGAAGACTTTGCCCCTGCGG
TGCTGGAAGGCGGGGCGAGCGCGGTGCTCGCCGCCTCGATCTTCCATTTCGGCGAGGCGAGTTTGGCAGAGGCGCGGGCT
GCGCTGGCCAAGGCCGGCGCGCCGGTGCGGACGGTCTAG

Upstream 100 bases:

>100_bases
TCCCGTTGCGTCAATATAGGCGCGAAACGCGCCCCTTCCCACCCCGGTAAACCTTACGGATTGTTTGCATTCGCGCGCCT
TTGGCGGCAAAGGAGCGGCC

Downstream 100 bases:

>100_bases
CCCCCTCCCCGCTTCCAGCGAGGGGGCTTGAAGAATTACTCCGCGAACACCGGTTTGCGCTTCTGCATTTCCGACATCAC
CGCTTCCATCTGGTTGGGCG

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLKTRIIPCLDVKDGRTVKGVNFVDLKDAGDPVALARAYDAAGADELCFLDITASHEGRGTLLETVSRTAEACFMPLTVG
GGVRSVEDMVALLRAGADKVAINSAAVADPALVSACAAKAGRQCVVVAIDARAVGDSWEIFTHGGRKATGINAVEFAREA
AKRGAGEILLTSMDRDGTKSGYDIPLLKAVSSAVTIPVIASGGAGSVEDFAPAVLEGGASAVLAASIFHFGEASLAEARA
ALAKAGAPVRTV

Sequences:

>Translated_252_residues
MLKTRIIPCLDVKDGRTVKGVNFVDLKDAGDPVALARAYDAAGADELCFLDITASHEGRGTLLETVSRTAEACFMPLTVG
GGVRSVEDMVALLRAGADKVAINSAAVADPALVSACAAKAGRQCVVVAIDARAVGDSWEIFTHGGRKATGINAVEFAREA
AKRGAGEILLTSMDRDGTKSGYDIPLLKAVSSAVTIPVIASGGAGSVEDFAPAVLEGGASAVLAASIFHFGEASLAEARA
ALAKAGAPVRTV
>Mature_252_residues
MLKTRIIPCLDVKDGRTVKGVNFVDLKDAGDPVALARAYDAAGADELCFLDITASHEGRGTLLETVSRTAEACFMPLTVG
GGVRSVEDMVALLRAGADKVAINSAAVADPALVSACAAKAGRQCVVVAIDARAVGDSWEIFTHGGRKATGINAVEFAREA
AKRGAGEILLTSMDRDGTKSGYDIPLLKAVSSAVTIPVIASGGAGSVEDFAPAVLEGGASAVLAASIFHFGEASLAEARA
ALAKAGAPVRTV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=43.75, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI87082028, Length=246, Percent_Identity=28.8617886178862, Blast_Score=87, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=32.2683706070287, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HYPNA (Q0C643)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758800.1
- ProteinModelPortal:   Q0C643
- SMR:   Q0C643
- STRING:   Q0C643
- GeneID:   4287275
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0066
- NMPDR:   fig|228405.5.peg.66
- TIGR:   HNE_0066
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q0C643
- ProtClustDB:   CLSK2531679
- BioCyc:   HNEP81032:HNE_0066-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 25580; Mature: 25580

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRIIPCLDVKDGRTVKGVNFVDLKDAGDPVALARAYDAAGADELCFLDITASHEGRG
CCCCCEEEEEECCCCCEECCCEEEEECCCCCCEEEHHHHCCCCCCCEEEEEEECCCCCCC
TLLETVSRTAEACFMPLTVGGGVRSVEDMVALLRAGADKVAINSAAVADPALVSACAAKA
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHHCCHHHHHHHHHHC
GRQCVVVAIDARAVGDSWEIFTHGGRKATGINAVEFAREAAKRGAGEILLTSMDRDGTKS
CCEEEEEEEECEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIPLLKAVSSAVTIPVIASGGAGSVEDFAPAVLEGGASAVLAASIFHFGEASLAEARA
CCCCHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
ALAKAGAPVRTV
HHHHCCCCCCCC
>Mature Secondary Structure
MLKTRIIPCLDVKDGRTVKGVNFVDLKDAGDPVALARAYDAAGADELCFLDITASHEGRG
CCCCCEEEEEECCCCCEECCCEEEEECCCCCCEEEHHHHCCCCCCCEEEEEEECCCCCCC
TLLETVSRTAEACFMPLTVGGGVRSVEDMVALLRAGADKVAINSAAVADPALVSACAAKA
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCHHHCCHHHHHHHHHHC
GRQCVVVAIDARAVGDSWEIFTHGGRKATGINAVEFAREAAKRGAGEILLTSMDRDGTKS
CCEEEEEEEECEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIPLLKAVSSAVTIPVIASGGAGSVEDFAPAVLEGGASAVLAASIFHFGEASLAEARA
CCCCHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
ALAKAGAPVRTV
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA