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The map label for this gene is eno

Identifier: 114797139

GI number: 114797139

Start: 2053244

End: 2054524

Strand: Reverse

Name: eno

Synonym: HNE_1980

Alternate gene names: 114797139

Gene position: 2054524-2053244 (Counterclockwise)

Preceding gene: 114798161

Following gene: 114797866

Centisome position: 55.45

GC content: 62.61

Gene sequence:

>1281_bases
ATGAGCGAAATCATCGACATTCACGCCCGCGAAATTCTCGACAGTCGCGGCAATCCGACGATCGAAGTCGATGTCACGCT
GGACGACGGCTCCACCGGCCGCGCCGCTGTGCCATCGGGCGCTTCGACAGGCGCCTATGAGGCCCACGAGCAGCGCGACG
GCGACAAGGCCCGCTATAACGGCAAGGGTGTCCTGAAAGCGGTTGACGCGGTCAATGGCGAGATCTGCAACGAGCTGGCC
GGCATGGACGCAACCGAACAGCGCCTCGTCGACACGCTGATGATCGACCTGGACGGCACCGAGAACAAATCCCGCCTCGG
CGCCAATGCCATTCTCGGCGTGTCGCTGGCCGTGGCCAAGGCAGCTGCCGAATCGTCTGCCCTGCCGCTCTACCGCTATC
TGGGCGGCGCCAATGCTCGCATCCTGCCGACCCCGATGATGAACATCATCAATGGCGGCGCGCACGCGGACAACCCGGTC
GACATCCAGGAATTCATGATCATGCCGGTCAGCGCCGAGAGCATCTCGGAAGCCGTCCGCATGGGCGCAGAAGTGTTCCA
CGCGCTGAAGAAGACGCTGCATGACGCTGGCCACAACACCAATGTCGGCGACGAAGGCGGCTTCGCGCCAAACCTTGCCT
CGGCCGACGAAGCCATCGGCTTCATCATGAAATCCATCGAGAAGGCAGGCTATCGCCCCGGCGAAGACATCGCGCTTGCC
CTCGATGCCGCCTCCACCGAGTTCTACAAGAACGGCAAGTATGAGCTGGCCGGCGAGGGCAAGTCGCTCTCGTCCGAACA
GTTCGGCCAATACCTGGCAGACCTCTGCGGCCGGTACCCGATTCTCTCCATCGAAGACGGCATGGCCGAAGACGATTGGG
ATGGCTGGGTCGCGCTGACCGAGCTCCTCGGCGACCGGGTTCAGCTCGTTGGCGACGATCTCTTCGTCACCAATCCTGAC
CGCCTTGCCATCGGCCTGCAAAAGGGCGCTGCCAACTCCATCCTCGTGAAGGTCAACCAGATCGGCACGCTGTCGGAAAC
GCTGGACGCGGTCGAACTGGCTCACCTGCACGGCTACACGGCCGTCATGTCCCACCGTTCGGGCGAAACCGAGGATTCGA
CGATTGCCGACCTCGCCGTGGCCACCAATTGCGGCCAGATCAAAACCGGCTCGCTCAGCCGGTCTGACCGGATCGCGAAA
TACAACCAGTTGATTCGGATCGAGGAAGAACTCGGCCCTATTGGCATGTATGCCGGCCTCGCGCGCATCAATGCGGGCTG
A

Upstream 100 bases:

>100_bases
TGCGCTCAGCAGCGCACCAGCCTAAGGGTTCCGGCGCCTCCTGCACCTTGCAGGGGGCGCTTTTCATTTGCACGACATAT
GCCTTTAAGGGGACGTTCCC

Downstream 100 bases:

>100_bases
GGCAGGTAGCCGAGCCTGCCTGGTTAAGAAACGGCTAAAGTCACACCGCAAACAGACCGAAGGGGTTAGCTAGCGGAACA
CGATGTTCTGCTAGACCTCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSEIIDIHAREILDSRGNPTIEVDVTLDDGSTGRAAVPSGASTGAYEAHEQRDGDKARYNGKGVLKAVDAVNGEICNELA
GMDATEQRLVDTLMIDLDGTENKSRLGANAILGVSLAVAKAAAESSALPLYRYLGGANARILPTPMMNIINGGAHADNPV
DIQEFMIMPVSAESISEAVRMGAEVFHALKKTLHDAGHNTNVGDEGGFAPNLASADEAIGFIMKSIEKAGYRPGEDIALA
LDAASTEFYKNGKYELAGEGKSLSSEQFGQYLADLCGRYPILSIEDGMAEDDWDGWVALTELLGDRVQLVGDDLFVTNPD
RLAIGLQKGAANSILVKVNQIGTLSETLDAVELAHLHGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRIAK
YNQLIRIEEELGPIGMYAGLARINAG

Sequences:

>Translated_426_residues
MSEIIDIHAREILDSRGNPTIEVDVTLDDGSTGRAAVPSGASTGAYEAHEQRDGDKARYNGKGVLKAVDAVNGEICNELA
GMDATEQRLVDTLMIDLDGTENKSRLGANAILGVSLAVAKAAAESSALPLYRYLGGANARILPTPMMNIINGGAHADNPV
DIQEFMIMPVSAESISEAVRMGAEVFHALKKTLHDAGHNTNVGDEGGFAPNLASADEAIGFIMKSIEKAGYRPGEDIALA
LDAASTEFYKNGKYELAGEGKSLSSEQFGQYLADLCGRYPILSIEDGMAEDDWDGWVALTELLGDRVQLVGDDLFVTNPD
RLAIGLQKGAANSILVKVNQIGTLSETLDAVELAHLHGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRIAK
YNQLIRIEEELGPIGMYAGLARINAG
>Mature_425_residues
SEIIDIHAREILDSRGNPTIEVDVTLDDGSTGRAAVPSGASTGAYEAHEQRDGDKARYNGKGVLKAVDAVNGEICNELAG
MDATEQRLVDTLMIDLDGTENKSRLGANAILGVSLAVAKAAAESSALPLYRYLGGANARILPTPMMNIINGGAHADNPVD
IQEFMIMPVSAESISEAVRMGAEVFHALKKTLHDAGHNTNVGDEGGFAPNLASADEAIGFIMKSIEKAGYRPGEDIALAL
DAASTEFYKNGKYELAGEGKSLSSEQFGQYLADLCGRYPILSIEDGMAEDDWDGWVALTELLGDRVQLVGDDLFVTNPDR
LAIGLQKGAANSILVKVNQIGTLSETLDAVELAHLHGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRIAKY
NQLIRIEEELGPIGMYAGLARINAG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=53.2710280373832, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=425, Percent_Identity=52, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=425, Percent_Identity=52, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=51.1682242990654, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=423, Percent_Identity=47.2813238770686, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=25.5882352941176, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=25.5882352941176, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=25.5882352941176, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.7715617715618, Blast_Score=513, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=51.6203703703704, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=51.6203703703704, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=47.6190476190476, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=49.4199535962877, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=48.9559164733179, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=48.9559164733179, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321693, Length=428, Percent_Identity=49.2990654205608, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=428, Percent_Identity=48.1308411214953, Blast_Score=365, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=51.0489510489511, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=51.0489510489511, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=51.0489510489511, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=51.0489510489511, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=51.0489510489511, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=51.0489510489511, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_HYPNA (Q0C0R3)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760680.1
- ProteinModelPortal:   Q0C0R3
- SMR:   Q0C0R3
- STRING:   Q0C0R3
- GeneID:   4288356
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1980
- NMPDR:   fig|228405.5.peg.1907
- TIGR:   HNE_1980
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q0C0R3
- ProtClustDB:   PRK00077
- BioCyc:   HNEP81032:HNE_1980-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45152; Mature: 45021

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00164 ENOLASE; PS00583 PFKB_KINASES_1

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIIDIHAREILDSRGNPTIEVDVTLDDGSTGRAAVPSGASTGAYEAHEQRDGDKARYN
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEC
GKGVLKAVDAVNGEICNELAGMDATEQRLVDTLMIDLDGTENKSRLGANAILGVSLAVAK
CCHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHHHCCHHHHHHHHHHHH
AAAESSALPLYRYLGGANARILPTPMMNIINGGAHADNPVDIQEFMIMPVSAESISEAVR
HHHCCCCCHHHHHHCCCCCEEECCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MGAEVFHALKKTLHDAGHNTNVGDEGGFAPNLASADEAIGFIMKSIEKAGYRPGEDIALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LDAASTEFYKNGKYELAGEGKSLSSEQFGQYLADLCGRYPILSIEDGMAEDDWDGWVALT
EECCCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
ELLGDRVQLVGDDLFVTNPDRLAIGLQKGAANSILVKVNQIGTLSETLDAVELAHLHGYT
HHHCCHHEEECCCEEEECCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHH
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRIAKYNQLIRIEEELGPIGMYAGL
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEHHHCCCHHHHHHH
ARINAG
HHCCCC
>Mature Secondary Structure 
SEIIDIHAREILDSRGNPTIEVDVTLDDGSTGRAAVPSGASTGAYEAHEQRDGDKARYN
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEC
GKGVLKAVDAVNGEICNELAGMDATEQRLVDTLMIDLDGTENKSRLGANAILGVSLAVAK
CCHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHHHCCHHHHHHHHHHHH
AAAESSALPLYRYLGGANARILPTPMMNIINGGAHADNPVDIQEFMIMPVSAESISEAVR
HHHCCCCCHHHHHHCCCCCEEECCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MGAEVFHALKKTLHDAGHNTNVGDEGGFAPNLASADEAIGFIMKSIEKAGYRPGEDIALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LDAASTEFYKNGKYELAGEGKSLSSEQFGQYLADLCGRYPILSIEDGMAEDDWDGWVALT
EECCCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
ELLGDRVQLVGDDLFVTNPDRLAIGLQKGAANSILVKVNQIGTLSETLDAVELAHLHGYT
HHHCCHHEEECCCEEEECCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHH
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRIAKYNQLIRIEEELGPIGMYAGL
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEHHHCCCHHHHHHH
ARINAG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA