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The map label for this gene is pleD [H]

Identifier: 114797095

GI number: 114797095

Start: 1540249

End: 1541190

Strand: Reverse

Name: pleD [H]

Synonym: HNE_1507

Alternate gene names: 114797095

Gene position: 1541190-1540249 (Counterclockwise)

Preceding gene: 114800094

Following gene: 114800265

Centisome position: 41.6

GC content: 57.96

Gene sequence:

>942_bases
ATGACAAGAGCAACGATCCTTGTTGTGGATGACGAAATCTCGAATATCGAGATCATTAATGCCATCCTTGAAGATCAGTA
TGAGGTATGCTTTGCGATCTCGGGTGCCCAGGCGCTCGAAACAATGCGCGAATTGCAGCCCGATCTCGTATTGCTCGATG
TGCTGATGCCGGAGATGGATGGGTTTGAGGTCTGTAAGCGGATCAAAAACGACCCTGATCTGGTGGACATTCCTGTTATC
TTCACCACGGGCCTTGGCGACACGGCCGATGAGATGAGGGGTCTTTCCCTCGGGGCCATTGATTATGTCACCAAGCCCAT
CCAGCCAGCGATTCTGCGGGCACGGGTTACCAACCATATGGAACTCAAAAGCTTGCGCGACCAGCTTGCCAATCTGGCCG
TTACCGATGCCCTGACCGGCCTCAAGAACCGGCGTTATCTGGAGCGCACCCTGCGTGCCGAATGCGCGCGGCTCGCCCGC
GATGAGGCCTGGCTTTCGGTTATCATGCTCGACATCGACTTCTTCAAACAGTTCAACGACACCTACGGCCATCCTGCCGG
GGATCGCTGCATTGCGATGATCGCTGCCACCCTCAAGCGCGTTGTGAAACGCGCCTCCGATACCCCCGCCCGCTATGGCG
GCGAAGAGTTTGCCTGTATCCTGCCCGATCTGGGCCCGGCCGAAGCAGAAATTGTTGCGCAGGAAATCCGCAGGCAGGTC
CACGCACTGAACATTCCGCATATACGCTCCCAGGCCGAGCCCTATGTGACCGTATCAATCGGCGTGGCCAGCGCCCGGTG
TCATAGCTCAATTGAGCCGGGCACCTGGATCACGTTCGCCGATCAGGAACTTTACCGCAGCAAGGCCGCCGGTCGCGACC
GGGTCTCCGTCATCGAATTTTCGGGCGACGCCTCCGGCATGGAAACCGGCACACATGGCTGA

Upstream 100 bases:

>100_bases
CCATTCTGAAAGCCGTGGAAAAACTCGACTACGACACGGCACTTAAACTCATTGAAAACCTGCAGTACGCGTACAATCCG
CGTGACGAAAGTGCTGCCTG

Downstream 100 bases:

>100_bases
CCCGGAGCTGTTGATTTCTACTGCTGAGAACTGCCCCTGGAAGGACAGACATCCATGCCTCGTAAAACCTGAAACAGGTC
TGGCGCGGGCTGCACCTGAT

Product: putative diguanylate cyclase/response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTRATILVVDDEISNIEIINAILEDQYEVCFAISGAQALETMRELQPDLVLLDVLMPEMDGFEVCKRIKNDPDLVDIPVI
FTTGLGDTADEMRGLSLGAIDYVTKPIQPAILRARVTNHMELKSLRDQLANLAVTDALTGLKNRRYLERTLRAECARLAR
DEAWLSVIMLDIDFFKQFNDTYGHPAGDRCIAMIAATLKRVVKRASDTPARYGGEEFACILPDLGPAEAEIVAQEIRRQV
HALNIPHIRSQAEPYVTVSIGVASARCHSSIEPGTWITFADQELYRSKAAGRDRVSVIEFSGDASGMETGTHG

Sequences:

>Translated_313_residues
MTRATILVVDDEISNIEIINAILEDQYEVCFAISGAQALETMRELQPDLVLLDVLMPEMDGFEVCKRIKNDPDLVDIPVI
FTTGLGDTADEMRGLSLGAIDYVTKPIQPAILRARVTNHMELKSLRDQLANLAVTDALTGLKNRRYLERTLRAECARLAR
DEAWLSVIMLDIDFFKQFNDTYGHPAGDRCIAMIAATLKRVVKRASDTPARYGGEEFACILPDLGPAEAEIVAQEIRRQV
HALNIPHIRSQAEPYVTVSIGVASARCHSSIEPGTWITFADQELYRSKAAGRDRVSVIEFSGDASGMETGTHG
>Mature_312_residues
TRATILVVDDEISNIEIINAILEDQYEVCFAISGAQALETMRELQPDLVLLDVLMPEMDGFEVCKRIKNDPDLVDIPVIF
TTGLGDTADEMRGLSLGAIDYVTKPIQPAILRARVTNHMELKSLRDQLANLAVTDALTGLKNRRYLERTLRAECARLARD
EAWLSVIMLDIDFFKQFNDTYGHPAGDRCIAMIAATLKRVVKRASDTPARYGGEEFACILPDLGPAEAEIVAQEIRRQVH
ALNIPHIRSQAEPYVTVSIGVASARCHSSIEPGTWITFADQELYRSKAAGRDRVSVIEFSGDASGMETGTHG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=182, Percent_Identity=35.1648351648352, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=183, Percent_Identity=36.0655737704918, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.1317365269461, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=38.0681818181818, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=35.1851851851852, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=189, Percent_Identity=34.3915343915344, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=31.055900621118, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=29.585798816568, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786599, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=46.1538461538462, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=37.1428571428571, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.1463414634146, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788394, Length=114, Percent_Identity=34.2105263157895, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787541, Length=182, Percent_Identity=29.1208791208791, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=32.03125, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=34.7457627118644, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=30.2521008403361, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789809, Length=112, Percent_Identity=30.3571428571429, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=118, Percent_Identity=30.5084745762712, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34604; Mature: 34473

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRATILVVDDEISNIEIINAILEDQYEVCFAISGAQALETMRELQPDLVLLDVLMPEMD
CCCEEEEEECCCCCCHHHHHHHHHCCHHEEEEECCHHHHHHHHHCCCCCEEHHHHHCCCC
GFEVCKRIKNDPDLVDIPVIFTTGLGDTADEMRGLSLGAIDYVTKPIQPAILRARVTNHM
HHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
ELKSLRDQLANLAVTDALTGLKNRRYLERTLRAECARLARDEAWLSVIMLDIDFFKQFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYGHPAGDRCIAMIAATLKRVVKRASDTPARYGGEEFACILPDLGPAEAEIVAQEIRRQV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCHHHHHHHHHHHHHH
HALNIPHIRSQAEPYVTVSIGVASARCHSSIEPGTWITFADQELYRSKAAGRDRVSVIEF
HHCCCCCHHCCCCCEEEEEEECCHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCEEEEEE
SGDASGMETGTHG
CCCCCCCCCCCCC
>Mature Secondary Structure 
TRATILVVDDEISNIEIINAILEDQYEVCFAISGAQALETMRELQPDLVLLDVLMPEMD
CCEEEEEECCCCCCHHHHHHHHHCCHHEEEEECCHHHHHHHHHCCCCCEEHHHHHCCCC
GFEVCKRIKNDPDLVDIPVIFTTGLGDTADEMRGLSLGAIDYVTKPIQPAILRARVTNHM
HHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
ELKSLRDQLANLAVTDALTGLKNRRYLERTLRAECARLARDEAWLSVIMLDIDFFKQFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYGHPAGDRCIAMIAATLKRVVKRASDTPARYGGEEFACILPDLGPAEAEIVAQEIRRQV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCHHHHHHHHHHHHHH
HALNIPHIRSQAEPYVTVSIGVASARCHSSIEPGTWITFADQELYRSKAAGRDRVSVIEF
HHCCCCCHHCCCCCEEEEEEECCHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCEEEEEE
SGDASGMETGTHG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA