Click here to switch to the map view.

The map label for this gene is nusB

Identifier: 114332465

GI number: 114332465

Start: 2646451

End: 2646954

Strand: Direct

Name: nusB

Synonym: Neut_2518

Alternate gene names: 114332465

Gene position: 2646451-2646954 (Clockwise)

Preceding gene: 114332464

Following gene: 114332466

Centisome position: 99.45

GC content: 49.6

Gene sequence:

>504_bases
ATGATACCAACAGACACTGCTCCACCATCCAAACCAGCACAAGGACACAAAGGCTATAAAAATCGCCGGCGTCTGTCACG
TGAACTGGCCTTGCAGGGAATTTACCAATGGCGTCTTGCCGGGGGAAATGCTCAGGAAATTGACCTGCAACTGCAACAGG
TCAATTTTTACAGCAAAGCGGATGGTTCCTACTTTTCCGATTTGCTGCAGGGTGTATTGGAACATTCCCGTGATCTGGAA
GCACAAATCCAAGCTTATCTTGATCGGCAGCTGGCAGAACTAAGTCCGGTGGAATGTTCGATTCTCCTAATTGGTACCTA
TGAAATGGTGCATCATCCTGAAATACCCTGTCGGGCTATCATCAATGAAGCGATAGAACTGGCCAAGAGTTATGGCGGGA
CAGACGGGCACAAATACGTCAACGGTGTACTGGACAAACTGGCCGCCCAGTTGCGGGCTGTTGAACTTCAATCCAGCCCC
GTCCGGAATAAGGATCCACACTAG

Upstream 100 bases:

>100_bases
CCCGTATGACCGAAAAAGGGGCTGAAACCGCGCGTGTCGCGGTAGAAATGGCCAATCTGGCATTAACGCTTGGCGAAATG
GATCAATCCACCACCTCTTC

Downstream 100 bases:

>100_bases
ATCCGGTCATTACGTCGATAGCAACACCTCTCTAAAAAATTGTTTAACCAAAACAGTCAACAGGAAAAGAGGGGGTGATT
TATTTTGCCATTGCAAACGC

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MIPTDTAPPSKPAQGHKGYKNRRRLSRELALQGIYQWRLAGGNAQEIDLQLQQVNFYSKADGSYFSDLLQGVLEHSRDLE
AQIQAYLDRQLAELSPVECSILLIGTYEMVHHPEIPCRAIINEAIELAKSYGGTDGHKYVNGVLDKLAAQLRAVELQSSP
VRNKDPH

Sequences:

>Translated_167_residues
MIPTDTAPPSKPAQGHKGYKNRRRLSRELALQGIYQWRLAGGNAQEIDLQLQQVNFYSKADGSYFSDLLQGVLEHSRDLE
AQIQAYLDRQLAELSPVECSILLIGTYEMVHHPEIPCRAIINEAIELAKSYGGTDGHKYVNGVLDKLAAQLRAVELQSSP
VRNKDPH
>Mature_167_residues
MIPTDTAPPSKPAQGHKGYKNRRRLSRELALQGIYQWRLAGGNAQEIDLQLQQVNFYSKADGSYFSDLLQGVLEHSRDLE
AQIQAYLDRQLAELSPVECSILLIGTYEMVHHPEIPCRAIINEAIELAKSYGGTDGHKYVNGVLDKLAAQLRAVELQSSP
VRNKDPH

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=125, Percent_Identity=40.8, Blast_Score=100, Evalue=5e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_NITEC (Q0AD60)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748687.1
- ProteinModelPortal:   Q0AD60
- SMR:   Q0AD60
- STRING:   Q0AD60
- GeneID:   4273372
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2518
- NMPDR:   fig|335283.3.peg.734
- eggNOG:   COG0781
- HOGENOM:   HBG734409
- OMA:   IPYKVVI
- PhylomeDB:   Q0AD60
- ProtClustDB:   CLSK586283
- BioCyc:   NEUT335283:NEUT_2518-MONOMER
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 18664; Mature: 18664

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPTDTAPPSKPAQGHKGYKNRRRLSRELALQGIYQWRLAGGNAQEIDLQLQQVNFYSKA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DGSYFSDLLQGVLEHSRDLEAQIQAYLDRQLAELSPVECSILLIGTYEMVHHPEIPCRAI
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHCCCCCHHHH
INEAIELAKSYGGTDGHKYVNGVLDKLAAQLRAVELQSSPVRNKDPH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIPTDTAPPSKPAQGHKGYKNRRRLSRELALQGIYQWRLAGGNAQEIDLQLQQVNFYSKA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DGSYFSDLLQGVLEHSRDLEAQIQAYLDRQLAELSPVECSILLIGTYEMVHHPEIPCRAI
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHCCCCCHHHH
INEAIELAKSYGGTDGHKYVNGVLDKLAAQLRAVELQSSPVRNKDPH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA