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The map label for this gene is gidB

Identifier: 114332388

GI number: 114332388

Start: 2550155

End: 2550799

Strand: Reverse

Name: gidB

Synonym: Neut_2436

Alternate gene names: 114332388

Gene position: 2550799-2550155 (Counterclockwise)

Preceding gene: 114332389

Following gene: 114332387

Centisome position: 95.86

GC content: 47.6

Gene sequence:

>645_bases
ATGAATCTGGAAAAACAGTTGCATGAAGGTCTGGAAGCAATTTCAGGGTTGTCCGCTGATGTGGCACATTTAAGCAGTCG
GTTATTACGCTACATTGAACTCATCACCAAGTGGAACAGTACTCATAATCTGACTTCGGTAAGAAATCCCGAATCGATGA
TTACTCGTCATATGCTGGACAGCCTGGTCGTATTACCACATATTAGTGGGCCAAATATTGTTGATGTCGGTAGTGGCGCC
GGCTTGCCGGGCATTCCCATTGCGCTGGCGCGTCCCGAATGGCAGGTGGTCATGGTAGAAAGTAACCAGAAGAAAGCTGT
TTTTATGCAACAGGTTGTGGTTGAGCTGCGTTTGCAGAATGTTTCAATCAGACAAGAGCGTGTGGAAAAAATAAAGCCGG
GAAACAAGGTGGATACTGTTATCTCACGCGCGTTTTCCAGTCTCGAACGTTTCATGCGCTTGTCGAAACATCTGTGTGAA
AACAACGTTGATCATTGTCGTTTCATTGCCATGAAAGGAGCGTTCCCGGATATGGAGTTGATGCAACTCCCTCCGGAATT
TTCCGTTGAGCAGATTATTCCCGTTGCGGTTCCTGGCCTGAGAGCCAAGCGTCATCTGGTCGTGATGCGGTGTCACTCCG
AATGA

Upstream 100 bases:

>100_bases
GCAGCACGGATTTCTGGTATTACACCGGCAGCCATATCCTTATTACTGGTACATCTAAAGCGTGGTATGGCACGCCAGGC
AGCCAAACAGGAAGAAACGC

Downstream 100 bases:

>100_bases
GATTTATTGAGTGGGATATAAGCGTATTCCATAATCTTCTTGCCAGAACAGAGACGTAATTTAATGGTCAGGATACTTGC
CATTGCCAACCAGAAGGGAG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNLEKQLHEGLEAISGLSADVAHLSSRLLRYIELITKWNSTHNLTSVRNPESMITRHMLDSLVVLPHISGPNIVDVGSGA
GLPGIPIALARPEWQVVMVESNQKKAVFMQQVVVELRLQNVSIRQERVEKIKPGNKVDTVISRAFSSLERFMRLSKHLCE
NNVDHCRFIAMKGAFPDMELMQLPPEFSVEQIIPVAVPGLRAKRHLVVMRCHSE

Sequences:

>Translated_214_residues
MNLEKQLHEGLEAISGLSADVAHLSSRLLRYIELITKWNSTHNLTSVRNPESMITRHMLDSLVVLPHISGPNIVDVGSGA
GLPGIPIALARPEWQVVMVESNQKKAVFMQQVVVELRLQNVSIRQERVEKIKPGNKVDTVISRAFSSLERFMRLSKHLCE
NNVDHCRFIAMKGAFPDMELMQLPPEFSVEQIIPVAVPGLRAKRHLVVMRCHSE
>Mature_214_residues
MNLEKQLHEGLEAISGLSADVAHLSSRLLRYIELITKWNSTHNLTSVRNPESMITRHMLDSLVVLPHISGPNIVDVGSGA
GLPGIPIALARPEWQVVMVESNQKKAVFMQQVVVELRLQNVSIRQERVEKIKPGNKVDTVISRAFSSLERFMRLSKHLCE
NNVDHCRFIAMKGAFPDMELMQLPPEFSVEQIIPVAVPGLRAKRHLVVMRCHSE

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=190, Percent_Identity=42.1052631578947, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_NITEC (Q0ADD7)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748610.1
- ProteinModelPortal:   Q0ADD7
- SMR:   Q0ADD7
- STRING:   Q0ADD7
- GeneID:   4273515
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2436
- NMPDR:   fig|335283.3.peg.1284
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   YRSAQHA
- PhylomeDB:   Q0ADD7
- ProtClustDB:   PRK00107
- BioCyc:   NEUT335283:NEUT_2436-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 24097; Mature: 24097

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKQLHEGLEAISGLSADVAHLSSRLLRYIELITKWNSTHNLTSVRNPESMITRHMLD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
SLVVLPHISGPNIVDVGSGAGLPGIPIALARPEWQVVMVESNQKKAVFMQQVVVELRLQN
HHHHEECCCCCCEEECCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHC
VSIRQERVEKIKPGNKVDTVISRAFSSLERFMRLSKHLCENNVDHCRFIAMKGAFPDMEL
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
MQLPPEFSVEQIIPVAVPGLRAKRHLVVMRCHSE
HHCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNLEKQLHEGLEAISGLSADVAHLSSRLLRYIELITKWNSTHNLTSVRNPESMITRHMLD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
SLVVLPHISGPNIVDVGSGAGLPGIPIALARPEWQVVMVESNQKKAVFMQQVVVELRLQN
HHHHEECCCCCCEEECCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHC
VSIRQERVEKIKPGNKVDTVISRAFSSLERFMRLSKHLCENNVDHCRFIAMKGAFPDMEL
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
MQLPPEFSVEQIIPVAVPGLRAKRHLVVMRCHSE
HHCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA