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The map label for this gene is pilT [H]

Identifier: 114332298

GI number: 114332298

Start: 2465042

End: 2466085

Strand: Reverse

Name: pilT [H]

Synonym: Neut_2338

Alternate gene names: 114332298

Gene position: 2466085-2465042 (Counterclockwise)

Preceding gene: 114332300

Following gene: 114332297

Centisome position: 92.67

GC content: 49.43

Gene sequence:

>1044_bases
ATGAACATAGTAGAACTACTCTCATTTGTGGTCAAGAATAGCGCTTCTGATCTGCATTTATCTGCTGGCATGCCACCCAT
GATTCGGGTGCATGGAGAGATTCGGCGTATCAATCTTCCCGCACTGGAGCACAAGGATGTGCATGACATGGTGTACGACA
TCATGAACGATAGCCAGCGTAAAGCTTATGAAGAACATCTGGAATGTGATTTTTCATTTGCTATTTCTGGTCTAGCTCGT
TTCCGAGTCAATGCCTTCAACACCCAACGGGGAGCAGCTTCGGTGCTGCGTACCATTCCATCCAAAGTACTAACGCTTGA
AGAGCTGAAAGCACCTAAGATATTTGCCGAGATCGCGCAACAGCCGCGTGGCGTGGTGTTGGTTACGGGGCCAACCGGAT
CTGGCAAGTCAACTACGCTTGCAGCCATGATCAATGATATTAACGAAAATCAGTATGGTCATATCCTGACTCTGGAAGAT
CCGATCGAATTTGTGCATGAAAGCAAAAAATGTCTGATTAACCAGCGTGAAGTCGGGCGAGATACGCACAGTTTCAGCAA
TGCACTGCGCTCTGCCTTACGGGAAGATCCAGATATTATTCTGGTGGGGGAAATGCGGGATTTGGAGACTATCCGCTTGG
CGATGACTGCTGCGGAAACCGGTCATCTGGTTTTCGGCACGCTACATACCAGTTCGGCTGCCAAGACGATTGACCGGATT
ATTGACGTGTTTCCTGCTGAAGAAAAGGAAATGGTACGTGCGATGTTGTCTGAATCGTTGCGTGCAGTTATTTCCCAGGC
GCTGCTGAAGACAAAGGATGGCAAAGGGCGAGTGGCCGCACACGAGATCATGATCGGTACACCGGCGATCCGTAACCTTA
TCCGGGAAGGGAAAGTGGCACAGATGTATTCAGCCATCCAGACCGGGCAGGGAGTCGGGATGCAGACACTTGATCAGAAT
CTGACAGATCTGGTGAAACGCGGTGTTATTTCGATAGCGGAAGCGCGCACCAAAGCGATGAACAAAGATAATTTCCGGGG
TTAA

Upstream 100 bases:

>100_bases
TGATCCGTTCTAGATTGGATCGGCCCGGTGTGGCCGGAAATGACATCCAACGATTGTCATTGACCAACCAATATTCCACG
AGGTAGAAGGAACACATCGG

Downstream 100 bases:

>100_bases
GGGTAGAACATGATGGAAAAAGAACAGGCACTTAAATTTATGCATGATTTATTGCGACTGATGCTAAGCAAGAAGGCTTC
AGACTTGTTCATCACAGCAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNIVELLSFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQRKAYEEHLECDFSFAISGLAR
FRVNAFNTQRGAASVLRTIPSKVLTLEELKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQGVGMQTLDQN
LTDLVKRGVISIAEARTKAMNKDNFRG

Sequences:

>Translated_347_residues
MNIVELLSFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQRKAYEEHLECDFSFAISGLAR
FRVNAFNTQRGAASVLRTIPSKVLTLEELKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQGVGMQTLDQN
LTDLVKRGVISIAEARTKAMNKDNFRG
>Mature_347_residues
MNIVELLSFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQRKAYEEHLECDFSFAISGLAR
FRVNAFNTQRGAASVLRTIPSKVLTLEELKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQGVGMQTLDQN
LTDLVKRGVISIAEARTKAMNKDNFRG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=328, Percent_Identity=47.8658536585366, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1789723, Length=344, Percent_Identity=31.3953488372093, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1786296, Length=309, Percent_Identity=31.7152103559871, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38323; Mature: 38323

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVELLSFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHCCHHH
KAYEEHLECDFSFAISGLARFRVNAFNTQRGAASVLRTIPSKVLTLEELKAPKIFAEIAQ
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLEDPIEFVHESKKCLINQREVGR
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHCCC
DTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEECCHHHHHHHHCCHHH
QMYSAIQTGQGVGMQTLDQNLTDLVKRGVISIAEARTKAMNKDNFRG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNIVELLSFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHCCHHH
KAYEEHLECDFSFAISGLARFRVNAFNTQRGAASVLRTIPSKVLTLEELKAPKIFAEIAQ
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLEDPIEFVHESKKCLINQREVGR
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHCCC
DTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEECCHHHHHHHHCCHHH
QMYSAIQTGQGVGMQTLDQNLTDLVKRGVISIAEARTKAMNKDNFRG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]