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The map label for this gene is pilT [H]

Identifier: 114332297

GI number: 114332297

Start: 2463893

End: 2465029

Strand: Reverse

Name: pilT [H]

Synonym: Neut_2337

Alternate gene names: 114332297

Gene position: 2465029-2463893 (Counterclockwise)

Preceding gene: 114332298

Following gene: 114332295

Centisome position: 92.63

GC content: 46.44

Gene sequence:

>1137_bases
ATGGAAAAAGAACAGGCACTTAAATTTATGCATGATTTATTGCGACTGATGCTAAGCAAGAAGGCTTCAGACTTGTTCAT
CACAGCAGGCTATCCTCCGGCGATGAAAATAGATGGAAAAATGACACCGGTCACGCAACAGGCGCTCTCTCCTATACATA
CCGAAGCTCTGGCACGGGTCATCATGAATAATAAGCAGGCGGCAGAGTTTGAGACCAGTAAGGAATGCAATTTTGCGATT
AATCCTGAGGGGATCGGGCGTTTTCGTGTTAATACCTTTGTCCAGCAACAGCGGACGGGCATCGTTTTGCGGACAATTAC
CACAAAGATCCCGGATTTCGATGATCTCGGGTTGCCACCGGTTCTGAAAGAGGTTGTCATGAGCAAACGCGGTCTGGTGG
TTTTTGTCGGTGGAACCGGTTCGGGAAAATCTACCTCGATGGCGGCGTTGATCGGCTATCGCAACAAAAGCAGCTATGGA
CACATCATTACCATTGAAGATCCGGTTGAATTTGTGCATGAGCATATCAACTGCGTCATTACTCAGCGGGAAGTCGGGGT
TGATACGGAATCCTGGGAAGCTGCGCTGAAGAATACGTTACGGCAGGCGCCTGATGTTATTTTGATTGGTGAAATCCGGG
ATCGGGAAACAATGGAACATGCCATTGCCTTTGCCGAGACCGGGCATTTATGCATGGGTACCTTGCACGCAAACAGTGCT
AATCAGGCGTTGGATCGCATTATCAACTTCTTTCCGGAAGAGCGGCGTGCGCAGTTGCTGATGGATTTATCACTGAATAT
GCGGGCATTGGTTGCACAAAGGTTAATTCCCAAAAAATCAGGCAGCGGGCGGGTTGCGGCAATCGAGATAATGCTCAATT
CTCCGTTGATTTCCGATCTGATATTCAAGGGAAATGTGCATGAAATCAAGGAAATCATGAAAAAATCAAGTGAATTGGGG
ATGCAGACCTTTGATATGGCGCTATTTGAACTTTACGAGAGTGGTGTGATTTCCTACGAAGATGCATTACGTAATGCTGA
TTCCGTGAATGAACTGCGACTGCAAATCAAACTGAACGGAGCAGAAGCAAAACGTATACATACAGAGACAAACATCGAAA
ATTTAACCATTACCTGA

Upstream 100 bases:

>100_bases
GAATCTGACAGATCTGGTGAAACGCGGTGTTATTTCGATAGCGGAAGCGCGCACCAAAGCGATGAACAAAGATAATTTCC
GGGGTTAAGGGTAGAACATG

Downstream 100 bases:

>100_bases
TTACACACTGTCGGTAATGATTCCTCCTCCCAGGCAGACTTTGCTTTCATAAATTACTACAGATTGCCCGGGAGTGACTC
CCCATTGCGGCGCTGCAAAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQALSPIHTEALARVIMNNKQAAEFETSKECNFAI
NPEGIGRFRVNTFVQQQRTGIVLRTITTKIPDFDDLGLPPVLKEVVMSKRGLVVFVGGTGSGKSTSMAALIGYRNKSSYG
HIITIEDPVEFVHEHINCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRVAAIEIMLNSPLISDLIFKGNVHEIKEIMKKSSELG
MQTFDMALFELYESGVISYEDALRNADSVNELRLQIKLNGAEAKRIHTETNIENLTIT

Sequences:

>Translated_378_residues
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQALSPIHTEALARVIMNNKQAAEFETSKECNFAI
NPEGIGRFRVNTFVQQQRTGIVLRTITTKIPDFDDLGLPPVLKEVVMSKRGLVVFVGGTGSGKSTSMAALIGYRNKSSYG
HIITIEDPVEFVHEHINCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRVAAIEIMLNSPLISDLIFKGNVHEIKEIMKKSSELG
MQTFDMALFELYESGVISYEDALRNADSVNELRLQIKLNGAEAKRIHTETNIENLTIT
>Mature_378_residues
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQALSPIHTEALARVIMNNKQAAEFETSKECNFAI
NPEGIGRFRVNTFVQQQRTGIVLRTITTKIPDFDDLGLPPVLKEVVMSKRGLVVFVGGTGSGKSTSMAALIGYRNKSSYG
HIITIEDPVEFVHEHINCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRVAAIEIMLNSPLISDLIFKGNVHEIKEIMKKSSELG
MQTFDMALFELYESGVISYEDALRNADSVNELRLQIKLNGAEAKRIHTETNIENLTIT

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=34.8909657320872, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=27.7777777777778, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789723, Length=155, Percent_Identity=38.7096774193548, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42136; Mature: 42136

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQALSPIHTEALARV
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCHHHHHHCHHHHHHHHHH
IMNNKQAAEFETSKECNFAINPEGIGRFRVNTFVQQQRTGIVLRTITTKIPDFDDLGLPP
HHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCH
VLKEVVMSKRGLVVFVGGTGSGKSTSMAALIGYRNKSSYGHIITIEDPVEFVHEHINCVI
HHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHEEE
TQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
EEHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCH
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRVAAIEIMLNSPLISDL
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHH
IFKGNVHEIKEIMKKSSELGMQTFDMALFELYESGVISYEDALRNADSVNELRLQIKLNG
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEEECC
AEAKRIHTETNIENLTIT
CCCCEEECCCCCCEEEEC
>Mature Secondary Structure
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQALSPIHTEALARV
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCHHHHHHCHHHHHHHHHH
IMNNKQAAEFETSKECNFAINPEGIGRFRVNTFVQQQRTGIVLRTITTKIPDFDDLGLPP
HHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCH
VLKEVVMSKRGLVVFVGGTGSGKSTSMAALIGYRNKSSYGHIITIEDPVEFVHEHINCVI
HHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHEEE
TQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
EEHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCH
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRVAAIEIMLNSPLISDL
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHH
IFKGNVHEIKEIMKKSSELGMQTFDMALFELYESGVISYEDALRNADSVNELRLQIKLNG
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEEECC
AEAKRIHTETNIENLTIT
CCCCEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]