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The map label for this gene is purN [H]

Identifier: 114332238

GI number: 114332238

Start: 2404773

End: 2405405

Strand: Direct

Name: purN [H]

Synonym: Neut_2278

Alternate gene names: 114332238

Gene position: 2404773-2405405 (Clockwise)

Preceding gene: 114332237

Following gene: 114332239

Centisome position: 90.37

GC content: 49.45

Gene sequence:

>633_bases
ATGAAATCAATGGTGATCCTTATTTCCGGTCGCGGTAGCAATATGCAAGCGTTACTGAAGGCCGGGCTACCAGTTGCAGC
AGTAATCAGCAATAATCCGACAGCAGAAGGATTGGCGTTTGCCCGCGAACACGGTATTCCAGCCCATGCGATTGATCATC
ATGCTTTCCCTGATCGGAAAACTTTTGATAATGCTCTGGCGGAAATCATTGATTCATACCAGCCTCACCTGGTTGCGCTC
GCCGGCTTCATGCGTATCCTGTCAGAAACATTTGTTGACCATTACCAGGGCAGGCTGATTAATATTCACCCCTCTCTGTT
GCCAGCATTCCCCGGACTGGATACCCATACACGAGCTCTGCAGGAAGGAGTCAAAATTCATGGTTGCACAGTTCATTTCG
TCACCTCGCAACTGGATCATGGCCCCATCATTATCCAAGCCGCCATACCGGTACTAGCTGATGATACGCCAGCAACACTG
GCGGCGAGAGTGCTGACGCAGGAACACCGCATTTATCCGCAAGCAGCAAACTGGTTTCTGCAGGGACAATTGACCTTGAC
AGAAAATCATGTTGAAACTGGAATGGCCTGTGATAGTCAGAAAGTTCTATATTCTCCCGGGATCACGTCATGA

Upstream 100 bases:

>100_bases
ATATCCTGCGCAGCGGTGGTGAAACCGTGTGGCAAATCGGTGATATCAAATCGCGTAAACCTGGAACGCCATCTATCGTC
ATTACCTGAAGGAAAGGATC

Downstream 100 bases:

>100_bases
AATTTTTATTACCCATAATCCTGTTCTGGATAAGCAGTATCACTTGCGCTGCTGATTTGCCTGAACGTATTGATATCGAC
TATGTACTCAACGGTCTTAT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKSMVILISGRGSNMQALLKAGLPVAAVISNNPTAEGLAFAREHGIPAHAIDHHAFPDRKTFDNALAEIIDSYQPHLVAL
AGFMRILSETFVDHYQGRLINIHPSLLPAFPGLDTHTRALQEGVKIHGCTVHFVTSQLDHGPIIIQAAIPVLADDTPATL
AARVLTQEHRIYPQAANWFLQGQLTLTENHVETGMACDSQKVLYSPGITS

Sequences:

>Translated_210_residues
MKSMVILISGRGSNMQALLKAGLPVAAVISNNPTAEGLAFAREHGIPAHAIDHHAFPDRKTFDNALAEIIDSYQPHLVAL
AGFMRILSETFVDHYQGRLINIHPSLLPAFPGLDTHTRALQEGVKIHGCTVHFVTSQLDHGPIIIQAAIPVLADDTPATL
AARVLTQEHRIYPQAANWFLQGQLTLTENHVETGMACDSQKVLYSPGITS
>Mature_210_residues
MKSMVILISGRGSNMQALLKAGLPVAAVISNNPTAEGLAFAREHGIPAHAIDHHAFPDRKTFDNALAEIIDSYQPHLVAL
AGFMRILSETFVDHYQGRLINIHPSLLPAFPGLDTHTRALQEGVKIHGCTVHFVTSQLDHGPIIIQAAIPVLADDTPATL
AARVLTQEHRIYPQAANWFLQGQLTLTENHVETGMACDSQKVLYSPGITS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=192, Percent_Identity=44.2708333333333, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=192, Percent_Identity=44.2708333333333, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=192, Percent_Identity=44.2708333333333, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=51.8134715025907, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1787483, Length=156, Percent_Identity=38.4615384615385, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=178, Percent_Identity=43.8202247191011, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=210, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=44.4444444444444, Blast_Score=158, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22776; Mature: 22776

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMVILISGRGSNMQALLKAGLPVAAVISNNPTAEGLAFAREHGIPAHAIDHHAFPDRK
CCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCCCCCH
TFDNALAEIIDSYQPHLVALAGFMRILSETFVDHYQGRLINIHPSLLPAFPGLDTHTRAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCHHHHHH
QEGVKIHGCTVHFVTSQLDHGPIIIQAAIPVLADDTPATLAARVLTQEHRIYPQAANWFL
HCCCEEECEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHCEEE
QGQLTLTENHVETGMACDSQKVLYSPGITS
EEEEEEECCCHHCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MKSMVILISGRGSNMQALLKAGLPVAAVISNNPTAEGLAFAREHGIPAHAIDHHAFPDRK
CCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCCCCCH
TFDNALAEIIDSYQPHLVALAGFMRILSETFVDHYQGRLINIHPSLLPAFPGLDTHTRAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCHHHHHH
QEGVKIHGCTVHFVTSQLDHGPIIIQAAIPVLADDTPATLAARVLTQEHRIYPQAANWFL
HCCCEEECEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHCEEE
QGQLTLTENHVETGMACDSQKVLYSPGITS
EEEEEEECCCHHCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]