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The map label for this gene is speB [H]

Identifier: 114332152

GI number: 114332152

Start: 2319470

End: 2320414

Strand: Direct

Name: speB [H]

Synonym: Neut_2187

Alternate gene names: 114332152

Gene position: 2319470-2320414 (Clockwise)

Preceding gene: 114332151

Following gene: 114332153

Centisome position: 87.16

GC content: 50.58

Gene sequence:

>945_bases
ATGAATACTTGGGATAACGGGTTTCGTGGCAAACCCGGACAGGGGATGTCGCATACTCTGAGTTTCGGGGGTGCAGCCAG
TTTTTTTCGCGTGCCTTACCAGGCAGATATCACAGGAGTTGATGTGGTCGTAACCGGAGTGCCGTTGGATATCGCCACCA
CCAATCGATCCGGCGCGCGACTGGGGCCTCGCGCCATTCGAAATGCATCGTTATCGCTGGCATGGGAGCGTCCATGGCCT
TGGAATATAGACCCGATGACTGCATTGCGTGTAATTGATTGTGGTGATTGCGAACCCGTTACTGACGATTTGTTGAGCGT
AGCGCATATCGAGGCACATATCGCCAATATTATCGGGGCAGGAGCAGCAGCCTTGGTGCTCGGTGGAGATCATTTTGTCG
CTTACCCAAGCCTGCGGGCACACGCCGCTGTGCATGGTCCTATCAGCCTGATTCATTTTGATGCCCATACTGATACTTGG
CCTTCCTCCGGTAATGGTATTAATCACGGCACTATGTTTTATCAGGCAACACATGAGGGAATTGTAAATCCAGCATCATC
CACACAAATTGGCATTCGCACCACTAATGATGACACACTGGGTTTTCATATCGTTTCCGCAGCAGATGTGCATCAAACGA
CCGGTCGCGACATAGCCAGCCAGATACACGAGCGAGTTGGTGAAAAACCGGTGTATATCACCTTTGATATCGATTGCTTG
GACCCAGCTTTTGCACCCGGCACTGGCACACCTGTACCGGGTGGACTGTCATCCTTCCAGGCACTGAGTATTATTCGTGA
ACTAAAAGGCATCAATCTGATAGGCATGGATGTCGTCGAAGTGGCGCCCGCGTATGATCATGCTGAAATTACTGCGCTAG
CGGCTGCACAGATTGCAGTTGAATTGTTATGTCTTTACGCTGAAAGTCACAGACAAAGAAATTGA

Upstream 100 bases:

>100_bases
GTGAAACCGGTTAGCTGGGAGTCCGAAGTTTCATCAAATGAAAACATGAAATCGAAAACAAAGATGGAATTACCGCGAAC
ACGCTATAGCAAGGAGATTT

Downstream 100 bases:

>100_bases
AAGAGTAATCGATGACCAATGAAGCAGTAGCAAAACACGATTGGTCCTGTGAGGATTCGCAGGCACTTTATGCCATGAGT
AAATGGGGAGATGGCTTTTA

Product: putative agmatinase

Products: NA

Alternate protein names: Agmatine ureohydrolase; AUH [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNTWDNGFRGKPGQGMSHTLSFGGAASFFRVPYQADITGVDVVVTGVPLDIATTNRSGARLGPRAIRNASLSLAWERPWP
WNIDPMTALRVIDCGDCEPVTDDLLSVAHIEAHIANIIGAGAAALVLGGDHFVAYPSLRAHAAVHGPISLIHFDAHTDTW
PSSGNGINHGTMFYQATHEGIVNPASSTQIGIRTTNDDTLGFHIVSAADVHQTTGRDIASQIHERVGEKPVYITFDIDCL
DPAFAPGTGTPVPGGLSSFQALSIIRELKGINLIGMDVVEVAPAYDHAEITALAAAQIAVELLCLYAESHRQRN

Sequences:

>Translated_314_residues
MNTWDNGFRGKPGQGMSHTLSFGGAASFFRVPYQADITGVDVVVTGVPLDIATTNRSGARLGPRAIRNASLSLAWERPWP
WNIDPMTALRVIDCGDCEPVTDDLLSVAHIEAHIANIIGAGAAALVLGGDHFVAYPSLRAHAAVHGPISLIHFDAHTDTW
PSSGNGINHGTMFYQATHEGIVNPASSTQIGIRTTNDDTLGFHIVSAADVHQTTGRDIASQIHERVGEKPVYITFDIDCL
DPAFAPGTGTPVPGGLSSFQALSIIRELKGINLIGMDVVEVAPAYDHAEITALAAAQIAVELLCLYAESHRQRN
>Mature_314_residues
MNTWDNGFRGKPGQGMSHTLSFGGAASFFRVPYQADITGVDVVVTGVPLDIATTNRSGARLGPRAIRNASLSLAWERPWP
WNIDPMTALRVIDCGDCEPVTDDLLSVAHIEAHIANIIGAGAAALVLGGDHFVAYPSLRAHAAVHGPISLIHFDAHTDTW
PSSGNGINHGTMFYQATHEGIVNPASSTQIGIRTTNDDTLGFHIVSAADVHQTTGRDIASQIHERVGEKPVYITFDIDCL
DPAFAPGTGTPVPGGLSSFQALSIIRELKGINLIGMDVVEVAPAYDHAEITALAAAQIAVELLCLYAESHRQRN

Specific function: Catalyzes the formation of putrescine from agmatine [H]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family. Agmatinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=291, Percent_Identity=41.9243986254296, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI10947139, Length=285, Percent_Identity=29.4736842105263, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4502215, Length=308, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789306, Length=287, Percent_Identity=51.219512195122, Blast_Score=273, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: =3.5.3.11 [H]

Molecular weight: Translated: 33412; Mature: 33412

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTWDNGFRGKPGQGMSHTLSFGGAASFFRVPYQADITGVDVVVTGVPLDIATTNRSGAR
CCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCEEEEEECCCEEEEECCCCCCC
LGPRAIRNASLSLAWERPWPWNIDPMTALRVIDCGDCEPVTDDLLSVAHIEAHIANIIGA
CCHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
GAAALVLGGDHFVAYPSLRAHAAVHGPISLIHFDAHTDTWPSSGNGINHGTMFYQATHEG
CEEEEEECCCEEEECCCHHHCEEECCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCC
IVNPASSTQIGIRTTNDDTLGFHIVSAADVHQTTGRDIASQIHERVGEKPVYITFDIDCL
CCCCCCCCEEEEEECCCCCEEEEEEEECHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEEC
DPAFAPGTGTPVPGGLSSFQALSIIRELKGINLIGMDVVEVAPAYDHAEITALAAAQIAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHCCCCCCHHHHHHHHHHHHH
ELLCLYAESHRQRN
HHHHHHHHHHCCCC
>Mature Secondary Structure
MNTWDNGFRGKPGQGMSHTLSFGGAASFFRVPYQADITGVDVVVTGVPLDIATTNRSGAR
CCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCEEEEEECCCEEEEECCCCCCC
LGPRAIRNASLSLAWERPWPWNIDPMTALRVIDCGDCEPVTDDLLSVAHIEAHIANIIGA
CCHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
GAAALVLGGDHFVAYPSLRAHAAVHGPISLIHFDAHTDTWPSSGNGINHGTMFYQATHEG
CEEEEEECCCEEEECCCHHHCEEECCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCC
IVNPASSTQIGIRTTNDDTLGFHIVSAADVHQTTGRDIASQIHERVGEKPVYITFDIDCL
CCCCCCCCEEEEEECCCCCEEEEEEEECHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEEC
DPAFAPGTGTPVPGGLSSFQALSIIRELKGINLIGMDVVEVAPAYDHAEITALAAAQIAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHCCCCCCHHHHHHHHHHHHH
ELLCLYAESHRQRN
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]