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The map label for this gene is nemA [H]

Identifier: 114332147

GI number: 114332147

Start: 2313529

End: 2314650

Strand: Direct

Name: nemA [H]

Synonym: Neut_2182

Alternate gene names: 114332147

Gene position: 2313529-2314650 (Clockwise)

Preceding gene: 114332143

Following gene: 114332148

Centisome position: 86.94

GC content: 53.39

Gene sequence:

>1122_bases
ATGACTACCACACTTTTTGATCCGTTTGATCTTTCCGGTCTGCAACTGGCTAACCGTATTGTCATGGCGCCGCTGACACG
CAACCGTTCACCCGGTGCTGTCCCGCAGCCCATCACTGCCACCTACTACGCACAGCGCGCCAGTGCCGGTCTGATCATCA
CCGAAGCAACGGCAATCTCTGCGCAAGGGCAAGGTTACGCCAACGTTCCTGGGCTTTATACGCAGCAAGCACTGGATGGC
TGGCGCACCATAACCGATATCGTACACACCAGGGGTGGCAAAATTATTGTACAGCTGTGGCACGTTGGCAGAATTTCACA
CAATAGCCTTCAGCCGGATAACCAGCCGCCAGTAGCACCTTCCGCCATTCGGGCACAGAGCACAACCTACTTAATTGATG
CCAATGGGCAGGGTAGTTTCGTACCCACTTCGCTACCGCGCGCACTGGCAGCCAGCGAACTGCCCGGCATTATTGATGAT
TACCGTCGTGCTGCGCGGGCAGCCATTGAAGTTGGCTTTGATGGTGTAGAGATTCATGCCGCCAACGGCTATCTGATTGA
TCAATTCCTGCGTTTGAGTTCCAACCAGCGCAGCGACAATTATGGCGGCAGCATTGAAAACCGTGCACGCTTCCTACTTG
CAGTGGTTGAAGGGGTCATGGAAGAAGTGGGTGCAACGTGCCCTGCTGGGATCCGGCTATCACCCGTCACTCCCGTTTGT
GATGTATTTGATCCTGAACCGCAACCACTATTTGAATATGTGGTACGCGGCCTTGCCAGGTATGCACTGTCTTATATCCA
CATTATTGAAGGAGCGACTGGCGGCGCCCGCGATCATCAACAGGGCAATACACCATTCGACTATGCCACCCTAAAGCAAG
TTTACCGCGATGCCGGTGGCAAAGCCGCCTGGATGCTCAATAACAACTACGATCCTGCACTTGCCCGTCAGGCGATTGAA
ACCGGCCAGGCAGATCTGATCTCTTTTGGCCGCCTTTTCATCAGCAACCCTGATCTGGTTGAACGACTACGGAAAAATTC
ACCCTTAACCACACCCGACACCAGTACTTTCTATGGCGGCAATGAACATGGTTATACGGATTATCCTTTTCTGGACGCGT
AG

Upstream 100 bases:

>100_bases
AACGTACACCCGTCTCCAGACGTAGAATACTAAGAAATATATCGATAGCCTTCTTCAACCGCCTGCTGATGTTTTAATTC
CCCCATCTCCGAGAAAAACC

Downstream 100 bases:

>100_bases
ATTATCAAGAGAGTTAAACTGTAGAAGATCAAACCTGTAACACAAGCACATAATTTCGTAGCAAAAGCGCGCAAACAGGC
GGATAGGGAAGAAACAGAGC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTTTLFDPFDLSGLQLANRIVMAPLTRNRSPGAVPQPITATYYAQRASAGLIITEATAISAQGQGYANVPGLYTQQALDG
WRTITDIVHTRGGKIIVQLWHVGRISHNSLQPDNQPPVAPSAIRAQSTTYLIDANGQGSFVPTSLPRALAASELPGIIDD
YRRAARAAIEVGFDGVEIHAANGYLIDQFLRLSSNQRSDNYGGSIENRARFLLAVVEGVMEEVGATCPAGIRLSPVTPVC
DVFDPEPQPLFEYVVRGLARYALSYIHIIEGATGGARDHQQGNTPFDYATLKQVYRDAGGKAAWMLNNNYDPALARQAIE
TGQADLISFGRLFISNPDLVERLRKNSPLTTPDTSTFYGGNEHGYTDYPFLDA

Sequences:

>Translated_373_residues
MTTTLFDPFDLSGLQLANRIVMAPLTRNRSPGAVPQPITATYYAQRASAGLIITEATAISAQGQGYANVPGLYTQQALDG
WRTITDIVHTRGGKIIVQLWHVGRISHNSLQPDNQPPVAPSAIRAQSTTYLIDANGQGSFVPTSLPRALAASELPGIIDD
YRRAARAAIEVGFDGVEIHAANGYLIDQFLRLSSNQRSDNYGGSIENRARFLLAVVEGVMEEVGATCPAGIRLSPVTPVC
DVFDPEPQPLFEYVVRGLARYALSYIHIIEGATGGARDHQQGNTPFDYATLKQVYRDAGGKAAWMLNNNYDPALARQAIE
TGQADLISFGRLFISNPDLVERLRKNSPLTTPDTSTFYGGNEHGYTDYPFLDA
>Mature_372_residues
TTTLFDPFDLSGLQLANRIVMAPLTRNRSPGAVPQPITATYYAQRASAGLIITEATAISAQGQGYANVPGLYTQQALDGW
RTITDIVHTRGGKIIVQLWHVGRISHNSLQPDNQPPVAPSAIRAQSTTYLIDANGQGSFVPTSLPRALAASELPGIIDDY
RRAARAAIEVGFDGVEIHAANGYLIDQFLRLSSNQRSDNYGGSIENRARFLLAVVEGVMEEVGATCPAGIRLSPVTPVCD
VFDPEPQPLFEYVVRGLARYALSYIHIIEGATGGARDHQQGNTPFDYATLKQVYRDAGGKAAWMLNNNYDPALARQAIET
GQADLISFGRLFISNPDLVERLRKNSPLTTPDTSTFYGGNEHGYTDYPFLDA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=373, Percent_Identity=45.5764075067024, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1789463, Length=344, Percent_Identity=31.9767441860465, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=269, Percent_Identity=27.8810408921933, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=273, Percent_Identity=25.2747252747253, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=153, Percent_Identity=32.0261437908497, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=149, Percent_Identity=30.8724832214765, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=139, Percent_Identity=33.8129496402878, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=170, Percent_Identity=27.0588235294118, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17566914, Length=276, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=37.0179948586118, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=35.73264781491, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40500; Mature: 40369

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTLFDPFDLSGLQLANRIVMAPLTRNRSPGAVPQPITATYYAQRASAGLIITEATAIS
CCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECEEEE
AQGQGYANVPGLYTQQALDGWRTITDIVHTRGGKIIVQLWHVGRISHNSLQPDNQPPVAP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
SAIRAQSTTYLIDANGQGSFVPTSLPRALAASELPGIIDDYRRAARAAIEVGFDGVEIHA
HHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEE
ANGYLIDQFLRLSSNQRSDNYGGSIENRARFLLAVVEGVMEEVGATCPAGIRLSPVTPVC
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHH
DVFDPEPQPLFEYVVRGLARYALSYIHIIEGATGGARDHQQGNTPFDYATLKQVYRDAGG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
KAAWMLNNNYDPALARQAIETGQADLISFGRLFISNPDLVERLRKNSPLTTPDTSTFYGG
CEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
NEHGYTDYPFLDA
CCCCCCCCCCCCC
>Mature Secondary Structure 
TTTLFDPFDLSGLQLANRIVMAPLTRNRSPGAVPQPITATYYAQRASAGLIITEATAIS
CCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECEEEE
AQGQGYANVPGLYTQQALDGWRTITDIVHTRGGKIIVQLWHVGRISHNSLQPDNQPPVAP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
SAIRAQSTTYLIDANGQGSFVPTSLPRALAASELPGIIDDYRRAARAAIEVGFDGVEIHA
HHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEE
ANGYLIDQFLRLSSNQRSDNYGGSIENRARFLLAVVEGVMEEVGATCPAGIRLSPVTPVC
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHH
DVFDPEPQPLFEYVVRGLARYALSYIHIIEGATGGARDHQQGNTPFDYATLKQVYRDAGG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
KAAWMLNNNYDPALARQAIETGQADLISFGRLFISNPDLVERLRKNSPLTTPDTSTFYGG
CEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
NEHGYTDYPFLDA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]