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The map label for this gene is gloB

Identifier: 114332143

GI number: 114332143

Start: 2307338

End: 2308105

Strand: Direct

Name: gloB

Synonym: Neut_2178

Alternate gene names: 114332143

Gene position: 2307338-2308105 (Clockwise)

Preceding gene: 114332139

Following gene: 114332147

Centisome position: 86.71

GC content: 48.83

Gene sequence:

>768_bases
ATGATCGATGTTTTCCCCGTCCGCGCCTTTAAAGACAATTATATCTGGATCGTTCACAATCAACAGTTTGCACTCATCAT
AGACCCTGGTGATGCAACACCAGTTCTCGCCTGGATAAGCCAACAGAACTTGCAACCAATTGCCATACTCTGCACGCATC
ACCATCATGATCACACGGGTGGCATTCCCTCACTGGTGCAGGAATTTGCGATACCTGTCTACGGCCCTGCCAACGAAAAA
ATACCGGGAATGACCCATCCATTAACGGGAGGAGATACACTCAGCTTTCCGGAATTGTCGCTGGAATTCAGCGTGCTGGA
TATTCCCGGACATACTGCTGGTCACATTGCTTACTATGGCCAGAATTACCTGTTTTGTGGAGATACACTGTTTGCATGCG
GCTGTGGGCGAATCTTTGAAGGCAGCGCGCAACAAATGCTTGCTTCTTTACAGAAACTGGCCAGTCTGCCGGATGAAACC
CTGGTTTATTGCGCTCATGAATACACGCTGGCCAATGTCCGGTTTGCCAGGGTACTCGACCCAGACAACCCTGATTTAAT
CAAACTGGAAAGCACTGTGGAAGAAAAACTGAAGCAGAATATACCCACACTCCCCTCCAGTCTGGCTGTCGAAAAAGCCA
CTAATCCGTTTTTGCGCTGTGATCAGCCAGCTATTATCCACAGCGCCAGCCAGCACGTTGGCCGGCATCTTAATGACCCA
GTCAGTGTTTTTGCTGCAATCCGTGACTGGAAAAATAACTTTCAGTAG

Upstream 100 bases:

>100_bases
AACCACTGTTGCATATTTTGCTGCAATGCAGAATCAAACATATTGTCAGAATGATTAGTTATGATGATGATTCGCTTGTC
ATTTACTGTAAGGAAAAAAA

Downstream 100 bases:

>100_bases
ACACCCCGTCATCGCGGTCACGAAGGCAATGATTACGTGGTGGCGAACTGCTTCATACCACCTTTTACAAAGGTAATTTA
AGCCAAAATATTTCCGCCTT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MIDVFPVRAFKDNYIWIVHNQQFALIIDPGDATPVLAWISQQNLQPIAILCTHHHHDHTGGIPSLVQEFAIPVYGPANEK
IPGMTHPLTGGDTLSFPELSLEFSVLDIPGHTAGHIAYYGQNYLFCGDTLFACGCGRIFEGSAQQMLASLQKLASLPDET
LVYCAHEYTLANVRFARVLDPDNPDLIKLESTVEEKLKQNIPTLPSSLAVEKATNPFLRCDQPAIIHSASQHVGRHLNDP
VSVFAAIRDWKNNFQ

Sequences:

>Translated_255_residues
MIDVFPVRAFKDNYIWIVHNQQFALIIDPGDATPVLAWISQQNLQPIAILCTHHHHDHTGGIPSLVQEFAIPVYGPANEK
IPGMTHPLTGGDTLSFPELSLEFSVLDIPGHTAGHIAYYGQNYLFCGDTLFACGCGRIFEGSAQQMLASLQKLASLPDET
LVYCAHEYTLANVRFARVLDPDNPDLIKLESTVEEKLKQNIPTLPSSLAVEKATNPFLRCDQPAIIHSASQHVGRHLNDP
VSVFAAIRDWKNNFQ
>Mature_255_residues
MIDVFPVRAFKDNYIWIVHNQQFALIIDPGDATPVLAWISQQNLQPIAILCTHHHHDHTGGIPSLVQEFAIPVYGPANEK
IPGMTHPLTGGDTLSFPELSLEFSVLDIPGHTAGHIAYYGQNYLFCGDTLFACGCGRIFEGSAQQMLASLQKLASLPDET
LVYCAHEYTLANVRFARVLDPDNPDLIKLESTVEEKLKQNIPTLPSSLAVEKATNPFLRCDQPAIIHSASQHVGRHLNDP
VSVFAAIRDWKNNFQ

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=265, Percent_Identity=36.9811320754717, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI94538320, Length=265, Percent_Identity=36.9811320754717, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI116642887, Length=228, Percent_Identity=38.5964912280702, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI21703352, Length=228, Percent_Identity=38.5964912280702, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI14150041, Length=264, Percent_Identity=34.0909090909091, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI46361987, Length=192, Percent_Identity=38.0208333333333, Blast_Score=120, Evalue=9e-28,
Organism=Escherichia coli, GI1786406, Length=248, Percent_Identity=47.1774193548387, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787158, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17536925, Length=267, Percent_Identity=34.0823970037453, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324614, Length=263, Percent_Identity=30.4182509505703, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320478, Length=248, Percent_Identity=28.6290322580645, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356335, Length=266, Percent_Identity=34.5864661654135, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24667711, Length=266, Percent_Identity=34.5864661654135, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24667703, Length=266, Percent_Identity=34.5864661654135, Blast_Score=158, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_NITEC (Q0AE32)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748365.1
- ProteinModelPortal:   Q0AE32
- SMR:   Q0AE32
- STRING:   Q0AE32
- GeneID:   4272775
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2178
- NMPDR:   fig|335283.3.peg.229
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q0AE32
- ProtClustDB:   CLSK585179
- BioCyc:   NEUT335283:NEUT_2178-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28233; Mature: 28233

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVFPVRAFKDNYIWIVHNQQFALIIDPGDATPVLAWISQQNLQPIAILCTHHHHDHTG
CCCEEEEEEECCCEEEEEECCEEEEEECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
GIPSLVQEFAIPVYGPANEKIPGMTHPLTGGDTLSFPELSLEFSVLDIPGHTAGHIAYYG
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEC
QNYLFCGDTLFACGCGRIFEGSAQQMLASLQKLASLPDETLVYCAHEYTLANVRFARVLD
CCEEEECCHHHHHCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEECEEEEEEEC
PDNPDLIKLESTVEEKLKQNIPTLPSSLAVEKATNPFLRCDQPAIIHSASQHVGRHLNDP
CCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCH
VSVFAAIRDWKNNFQ
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDVFPVRAFKDNYIWIVHNQQFALIIDPGDATPVLAWISQQNLQPIAILCTHHHHDHTG
CCCEEEEEEECCCEEEEEECCEEEEEECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
GIPSLVQEFAIPVYGPANEKIPGMTHPLTGGDTLSFPELSLEFSVLDIPGHTAGHIAYYG
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEC
QNYLFCGDTLFACGCGRIFEGSAQQMLASLQKLASLPDETLVYCAHEYTLANVRFARVLD
CCEEEECCHHHHHCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEECEEEEEEEC
PDNPDLIKLESTVEEKLKQNIPTLPSSLAVEKATNPFLRCDQPAIIHSASQHVGRHLNDP
CCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCH
VSVFAAIRDWKNNFQ
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA