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The map label for this gene is queF

Identifier: 114332088

GI number: 114332088

Start: 2251148

End: 2251567

Strand: Direct

Name: queF

Synonym: Neut_2123

Alternate gene names: 114332088

Gene position: 2251148-2251567 (Clockwise)

Preceding gene: 114332086

Following gene: 114332089

Centisome position: 84.6

GC content: 45.71

Gene sequence:

>420_bases
ATGACCACACAGCCTTCAAAGCAACTGGAAACCTTTGAAAACCCTATTCAGACACGCGATTACCGGATTCACATGGAAAT
ACCGGAGTTTACCTGCTTGTGCCCAAAAACCGGGCAACCAGATTTTGCGCGACTAACGCTGGATTACATTCCGGATAAAA
AATGTATCGAACTCAAGAGTCTCAAGCTCTATATCTGGTCGTATCGCAATGAGGGTACTTTTCATGAAGCAGTCACCAAC
CAGATTCTGGATGATCTGGTTATTGCCATGAAACCCCGCTTTATTCGGCTGACCTCAAAATTTTACGTCCGTGGCGGAAT
TTTTACCAATGTCGTTGCAGAACATCGCAAGAAAGGCTGGCACCCTCAGCCTCCGGTATTTCTGGAATCCTTCGATGAAC
AGTTCAATACCCGTAGCTAA

Upstream 100 bases:

>100_bases
AATTTGGAAGGCAAGAGGTTATAATTTCAGGTTGCCTGGTTATTTTAAATGATTTACCTGTCATAAAGAAGAACCGAAAA
TCGGGAGAACTGAGGAAACT

Downstream 100 bases:

>100_bases
TCGCTTTCTTTCATACAACTCGTCAATCTCTCTCGGGAAATAGCCATAATGGATTCGTATCGCGAAGAACATGATGCAAT
GGGTACGGTGCAAGTACCTG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MTTQPSKQLETFENPIQTRDYRIHMEIPEFTCLCPKTGQPDFARLTLDYIPDKKCIELKSLKLYIWSYRNEGTFHEAVTN
QILDDLVIAMKPRFIRLTSKFYVRGGIFTNVVAEHRKKGWHPQPPVFLESFDEQFNTRS

Sequences:

>Translated_139_residues
MTTQPSKQLETFENPIQTRDYRIHMEIPEFTCLCPKTGQPDFARLTLDYIPDKKCIELKSLKLYIWSYRNEGTFHEAVTN
QILDDLVIAMKPRFIRLTSKFYVRGGIFTNVVAEHRKKGWHPQPPVFLESFDEQFNTRS
>Mature_138_residues
TTQPSKQLETFENPIQTRDYRIHMEIPEFTCLCPKTGQPDFARLTLDYIPDKKCIELKSLKLYIWSYRNEGTFHEAVTNQ
ILDDLVIAMKPRFIRLTSKFYVRGGIFTNVVAEHRKKGWHPQPPVFLESFDEQFNTRS

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_NITEC (Q0AE87)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748310.1
- ProteinModelPortal:   Q0AE87
- SMR:   Q0AE87
- STRING:   Q0AE87
- GeneID:   4274165
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2123
- NMPDR:   fig|335283.3.peg.149
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- BioCyc:   NEUT335283:NEUT_2123-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 16429; Mature: 16298

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQPSKQLETFENPIQTRDYRIHMEIPEFTCLCPKTGQPDFARLTLDYIPDKKCIELKS
CCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCCEEHEE
LKLYIWSYRNEGTFHEAVTNQILDDLVIAMKPRFIRLTSKFYVRGGIFTNVVAEHRKKGW
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCCC
HPQPPVFLESFDEQFNTRS
CCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTQPSKQLETFENPIQTRDYRIHMEIPEFTCLCPKTGQPDFARLTLDYIPDKKCIELKS
CCCCHHHHHHHCCCCCCCCEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCCEEHEE
LKLYIWSYRNEGTFHEAVTNQILDDLVIAMKPRFIRLTSKFYVRGGIFTNVVAEHRKKGW
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCCC
HPQPPVFLESFDEQFNTRS
CCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA