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The map label for this gene is lpdA [H]

Identifier: 114332068

GI number: 114332068

Start: 2226096

End: 2227865

Strand: Reverse

Name: lpdA [H]

Synonym: Neut_2103

Alternate gene names: 114332068

Gene position: 2227865-2226096 (Counterclockwise)

Preceding gene: 114332069

Following gene: 114332065

Centisome position: 83.72

GC content: 50.9

Gene sequence:

>1770_bases
ATGACACAATTTACAACAATAGTTTTGCCTGATATTGGCGATTTTAAGGATGTTCCAGTCATAGAAACTCTGGTTAAACC
GGGGGATCGGGTAGAACAGGAAATGCCGCTGTTGGTACTCGAGACCGATAAAGCTTCGATAGAAGTTCCGGCGCCTCAGT
CGGGCATCGTGAAAGAAATGCACGTCAAAGCCGGTGATAAGATTTCACAAGGATCGCTCATTGCCACGCTGGAAGTCAAG
GACGCGGAGGCTGTGCCTTCAGCATCATCCTATGATGAGACTAAAGTTACCGATCAACCCGTTGTTGCAGGTGCAGGCAA
AGTACCTTCCAGGATAGTGGTGCCGTCACCTGAAACTGCGCGTGGCAAGTTACGTGCTGAAGTGGTTGTGCTGGGTGCCG
GTCCGGGAGGGTATACGGCAGCGTTTCGTGCGGCCGATTTGGGTAAACAAGTGGTGTTGATTGAGCGTTATTCCACATTG
GGAGGGGTTTGTCTGAATGTGGGTTGCATTCCCTCCAAGGCACTGTTGCATGCTGCAAAAACGCTTACCGAAGCAAAAGA
GGCGGATCAGTACGGTATTCATTTCGGAAGGCCGGAAATTGATATTGGCAAACTGTGTTCCTGGAAAGAATCGGTTGTGG
GCAAGCTGACCAAGGGATTATCGATGCTGGCTAAGCAACGCAAGGTGACCGTTATTCATGGCGCCGGGAAGTTTGCCAAT
TCGCATCTAATTGAAGTTGAAACATCGGATGGGATCGAAACAGTTTCATTTGAGCATTGCATTATTGCGGCCGGATCCAG
CGCTGCACGCATCCCGATATTACCGGATAACGAATGCATTATTGATTCGACCGGCGCACTGGCATTGACGGAGATCCCGC
CGCGCATGCTTATTGTGGGAGGTGGAGTCATCGGGTTGGAGATGGCTACGGTCTATCATGCACTGGGTAGCAAAATCAGT
ATTGTGGAACAGATGGTGCAGCTTATTCCAGGCGCCGATGCTGATCTGGTTAAACCGCTTCATAAGAGACTGAAAGCTGG
ATGTGAGGCGATTTACCTCAATACCAGTGTCAGTCGGATTGAAGTGGACGGTAAGGAGGTGCAGGTGTTTTTTGAAGGCG
AGCAGGCACCGGAGCCACAACGCTATGATCGAGTGTTGGTTGCGGTTGGGCGTCGACCTAACGGAAAGCTGATCAATGCT
GAGGCAGCGGGTGTGAGCGTGGATGAGCACGGTTTCATCACGGTGGATAAGCAGATGCGAACCAATATTGCTCATATTTT
TGCAATTGGCGATATTGTTGGAGATCCTATGCTGGCACACAAGGCCTCACATGAAGGCAAAATAGCAGCAGAAGTTATTG
CCGGGCACAAGGTGGCATTCGATGCGCGCACCATTCCTTCGGTGGCCTATACCGATCCTGAAGTTGCCTGGATGGGACTG
ACAGAGACCGAAGCCGAAAAACAGGGTATTGCTTATGAGAAAGCAGTATTTCCCTGGGCAGCTAGTGGTCGTGCCATCAC
CCTGGCGCGTGATGAAGGCATGACCAAATTACTCTTTGACAAGACTACCCGAAGAATTCTTGGTGCAGGAATTGTGGGTG
CTCATGCAGGGGAGTTGATCTCGGAAACAGTGCTGGCGCTGGAAATGGGGGCGGATATGCAGGATATCGGGCTGACTATC
CATCCCCATCCCACTTTATCCGAAACCGTCCTTTTTTCCGCAGAACTGGCTGAGGGTACGATCACGGATCTCTACGTGCC
GAAGAAGTGA

Upstream 100 bases:

>100_bases
TCTACAGATAAGTTGCACGCACGTGGCCCAGTGGGGCTGGAAGGACTGACCAGCCAGAAGTTTATCGTGCTGGGTGATGG
CCATATCAGGGAATAAGAAG

Downstream 100 bases:

>100_bases
ATGTAGAGCAGTAACTGGCTGGCTGTTTTTTCTGTTTGGCCAGCCAGATAGAAAATATCCAGAGAATCAGAAATGCAGGA
TAGCACTGGCCGAGAGCAGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTQFTTIVLPDIGDFKDVPVIETLVKPGDRVEQEMPLLVLETDKASIEVPAPQSGIVKEMHVKAGDKISQGSLIATLEVK
DAEAVPSASSYDETKVTDQPVVAGAGKVPSRIVVPSPETARGKLRAEVVVLGAGPGGYTAAFRAADLGKQVVLIERYSTL
GGVCLNVGCIPSKALLHAAKTLTEAKEADQYGIHFGRPEIDIGKLCSWKESVVGKLTKGLSMLAKQRKVTVIHGAGKFAN
SHLIEVETSDGIETVSFEHCIIAAGSSAARIPILPDNECIIDSTGALALTEIPPRMLIVGGGVIGLEMATVYHALGSKIS
IVEQMVQLIPGADADLVKPLHKRLKAGCEAIYLNTSVSRIEVDGKEVQVFFEGEQAPEPQRYDRVLVAVGRRPNGKLINA
EAAGVSVDEHGFITVDKQMRTNIAHIFAIGDIVGDPMLAHKASHEGKIAAEVIAGHKVAFDARTIPSVAYTDPEVAWMGL
TETEAEKQGIAYEKAVFPWAASGRAITLARDEGMTKLLFDKTTRRILGAGIVGAHAGELISETVLALEMGADMQDIGLTI
HPHPTLSETVLFSAELAEGTITDLYVPKK

Sequences:

>Translated_589_residues
MTQFTTIVLPDIGDFKDVPVIETLVKPGDRVEQEMPLLVLETDKASIEVPAPQSGIVKEMHVKAGDKISQGSLIATLEVK
DAEAVPSASSYDETKVTDQPVVAGAGKVPSRIVVPSPETARGKLRAEVVVLGAGPGGYTAAFRAADLGKQVVLIERYSTL
GGVCLNVGCIPSKALLHAAKTLTEAKEADQYGIHFGRPEIDIGKLCSWKESVVGKLTKGLSMLAKQRKVTVIHGAGKFAN
SHLIEVETSDGIETVSFEHCIIAAGSSAARIPILPDNECIIDSTGALALTEIPPRMLIVGGGVIGLEMATVYHALGSKIS
IVEQMVQLIPGADADLVKPLHKRLKAGCEAIYLNTSVSRIEVDGKEVQVFFEGEQAPEPQRYDRVLVAVGRRPNGKLINA
EAAGVSVDEHGFITVDKQMRTNIAHIFAIGDIVGDPMLAHKASHEGKIAAEVIAGHKVAFDARTIPSVAYTDPEVAWMGL
TETEAEKQGIAYEKAVFPWAASGRAITLARDEGMTKLLFDKTTRRILGAGIVGAHAGELISETVLALEMGADMQDIGLTI
HPHPTLSETVLFSAELAEGTITDLYVPKK
>Mature_588_residues
TQFTTIVLPDIGDFKDVPVIETLVKPGDRVEQEMPLLVLETDKASIEVPAPQSGIVKEMHVKAGDKISQGSLIATLEVKD
AEAVPSASSYDETKVTDQPVVAGAGKVPSRIVVPSPETARGKLRAEVVVLGAGPGGYTAAFRAADLGKQVVLIERYSTLG
GVCLNVGCIPSKALLHAAKTLTEAKEADQYGIHFGRPEIDIGKLCSWKESVVGKLTKGLSMLAKQRKVTVIHGAGKFANS
HLIEVETSDGIETVSFEHCIIAAGSSAARIPILPDNECIIDSTGALALTEIPPRMLIVGGGVIGLEMATVYHALGSKISI
VEQMVQLIPGADADLVKPLHKRLKAGCEAIYLNTSVSRIEVDGKEVQVFFEGEQAPEPQRYDRVLVAVGRRPNGKLINAE
AAGVSVDEHGFITVDKQMRTNIAHIFAIGDIVGDPMLAHKASHEGKIAAEVIAGHKVAFDARTIPSVAYTDPEVAWMGLT
ETEAEKQGIAYEKAVFPWAASGRAITLARDEGMTKLLFDKTTRRILGAGIVGAHAGELISETVLALEMGADMQDIGLTIH
PHPTLSETVLFSAELAEGTITDLYVPKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=40.084388185654, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=27.4725274725275, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=27.3127753303965, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=27.7533039647577, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=27.3127753303965, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=27.3127753303965, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=27.3127753303965, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI291045266, Length=473, Percent_Identity=25.7928118393235, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.1659388646288, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI291045268, Length=467, Percent_Identity=24.6252676659529, Blast_Score=107, Evalue=5e-23,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=58.1196581196581, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=30.4157549234136, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=27.6688453159041, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=30.0925925925926, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786305, Length=71, Percent_Identity=57.7464788732394, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32565766, Length=449, Percent_Identity=39.8663697104677, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17557007, Length=484, Percent_Identity=31.404958677686, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=25.5707762557078, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=438, Percent_Identity=25.5707762557078, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=461, Percent_Identity=24.9457700650759, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.8859649122807, Blast_Score=302, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=30.3609341825902, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=447, Percent_Identity=26.3982102908277, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21358499, Length=452, Percent_Identity=42.0353982300885, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=28.39248434238, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=28.39248434238, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=28.4823284823285, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=27.4949083503055, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 62635; Mature: 62504

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFTTIVLPDIGDFKDVPVIETLVKPGDRVEQEMPLLVLETDKASIEVPAPQSGIVKEM
CCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHCCEEEEECCCCEEECCCCCCCCHHHH
HVKAGDKISQGSLIATLEVKDAEAVPSASSYDETKVTDQPVVAGAGKVPSRIVVPSPETA
HHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCC
RGKLRAEVVVLGAGPGGYTAAFRAADLGKQVVLIERYSTLGGVCLNVGCIPSKALLHAAK
CCCEEEEEEEEECCCCCCHHHEEHHHCCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHH
TLTEAKEADQYGIHFGRPEIDIGKLCSWKESVVGKLTKGLSMLAKQRKVTVIHGAGKFAN
HHHHHHHCCCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
SHLIEVETSDGIETVSFEHCIIAAGSSAARIPILPDNECIIDSTGALALTEIPPRMLIVG
CEEEEEECCCCCEEEEECEEEEECCCCCCEEEECCCCCEEEECCCCEEEECCCCEEEEEE
GGVIGLEMATVYHALGSKISIVEQMVQLIPGADADLVKPLHKRLKAGCEAIYLNTSVSRI
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCEEEE
EVDGKEVQVFFEGEQAPEPQRYDRVLVAVGRRPNGKLINAEAAGVSVDEHGFITVDKQMR
EECCCEEEEEECCCCCCCCHHHCEEEEEECCCCCCCEEECCCCCCEECCCCEEEECHHHH
TNIAHIFAIGDIVGDPMLAHKASHEGKIAAEVIAGHKVAFDARTIPSVAYTDPEVAWMGL
CCEEEEEEEHHHHCCCHHHHCCCCCCCEEEEECCCCEEEECCCCCCCEEECCCCEEEEEC
TETEAEKQGIAYEKAVFPWAASGRAITLARDEGMTKLLFDKTTRRILGAGIVGAHAGELI
CCCHHHHCCCEEHHEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHH
SETVLALEMGADMQDIGLTIHPHPTLSETVLFSAELAEGTITDLYVPKK
HHHHHHHHCCCCHHHCCEEECCCCCCCHHEEEEEHHCCCCEEEEEECCC
>Mature Secondary Structure 
TQFTTIVLPDIGDFKDVPVIETLVKPGDRVEQEMPLLVLETDKASIEVPAPQSGIVKEM
CCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHCCEEEEECCCCEEECCCCCCCCHHHH
HVKAGDKISQGSLIATLEVKDAEAVPSASSYDETKVTDQPVVAGAGKVPSRIVVPSPETA
HHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCC
RGKLRAEVVVLGAGPGGYTAAFRAADLGKQVVLIERYSTLGGVCLNVGCIPSKALLHAAK
CCCEEEEEEEEECCCCCCHHHEEHHHCCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHH
TLTEAKEADQYGIHFGRPEIDIGKLCSWKESVVGKLTKGLSMLAKQRKVTVIHGAGKFAN
HHHHHHHCCCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
SHLIEVETSDGIETVSFEHCIIAAGSSAARIPILPDNECIIDSTGALALTEIPPRMLIVG
CEEEEEECCCCCEEEEECEEEEECCCCCCEEEECCCCCEEEECCCCEEEECCCCEEEEEE
GGVIGLEMATVYHALGSKISIVEQMVQLIPGADADLVKPLHKRLKAGCEAIYLNTSVSRI
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCEEEE
EVDGKEVQVFFEGEQAPEPQRYDRVLVAVGRRPNGKLINAEAAGVSVDEHGFITVDKQMR
EECCCEEEEEECCCCCCCCHHHCEEEEEECCCCCCCEEECCCCCCEECCCCEEEECHHHH
TNIAHIFAIGDIVGDPMLAHKASHEGKIAAEVIAGHKVAFDARTIPSVAYTDPEVAWMGL
CCEEEEEEEHHHHCCCHHHHCCCCCCCEEEEECCCCEEEECCCCCCCEEECCCCEEEEEC
TETEAEKQGIAYEKAVFPWAASGRAITLARDEGMTKLLFDKTTRRILGAGIVGAHAGELI
CCCHHHHCCCEEHHEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHH
SETVLALEMGADMQDIGLTIHPHPTLSETVLFSAELAEGTITDLYVPKK
HHHHHHHHCCCCHHHCCEEECCCCCCCHHEEEEEHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]