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The map label for this gene is bepF [H]

Identifier: 114331919

GI number: 114331919

Start: 2058495

End: 2059559

Strand: Direct

Name: bepF [H]

Synonym: Neut_1946

Alternate gene names: 114331919

Gene position: 2058495-2059559 (Clockwise)

Preceding gene: 114331918

Following gene: 114331920

Centisome position: 77.36

GC content: 45.07

Gene sequence:

>1065_bases
ATGGTTACGCCGGTTATTCAGGAAAAAGTCGCTAAAATTCATGAACTGGTGGGTCGTACCGCCGCACTCAGAACGGTAAA
TCTAGTGGCTCGTGTGCAAGGCTTTCTGGAAAGACGGAATTTCATAGAAGGAGATAGCGTTAAAGAGGGTGATATTTTAT
ACGTGATAGAGCAAGCGCCTTATCAAATCGAAGTTAAGGCTGCACAAGCGAAAGTAGCCGAAGCTAAAGCTACTCTACAG
AATGCTAAATCTTATTTGCAACGATTAAAAACGGTGCGCCAAGGCGGAGTGTCACAAATGGACCTTGATAAAGCCAGCAG
TGATTTTCTTAGCGCACAAGCGCTGTTAACCAGTGCCCAGGCCATGTTGGACCGAGCTAAATTGAATCTCAGTTACACTG
AAATACGTTCTCCGATCAATGGGCGCATCGGTCGTAATTCTATCAGCACGGGAAACCTTGTCTCACCAAACTCCGGCATT
TTAGCAACAATTGTGCTAATCGACCCCCTGTATGTACTTTTTACGGTGAGCGAAGTTGATTTCCTTACCGAGCTTCAAAA
CCAGCTGGAGAAGGGAGAGTCGACTATATTTGTTCCTAAAATTCAACTTGCCAATGGAACCATTTATCCTTTTGAAGGAA
AATCGGATTTTATCAGCCCCATCGTGGATGAAAAGACTGGAACTATCACGATTCGAGCAATTTTCCCAAACCCCGATACA
AAAGAATTACTCGGTCAGACAAATACGCCACAAAGCCGCCGCTTACTGATGCCGGGACAATTTGTTAAAGTTTTGGTAAG
ACGAGATGATGTAGAGGAAGAACATGTTATTCCCCAGTCGGCTATGCAGGAAGATCAAAGTGGGAAATATGTGCTGGTTG
TTGATGCTGACAACCGCGTGCAAAAACGCTCAGTGACAGTCGGTGACAAAATCGGCGTAAATTGGATCATTAAGCGAGGT
TTGCAGGTGGGCGAATTAGTGATATCTGAGGGTGCGCAAAAGGTTCGCCCCGGCATTACTGTACAAACTACCATAGCGGT
TCCTTCTCAACCGATCGAGAAATAA

Upstream 100 bases:

>100_bases
TACTTCTCTTCTAGGTCTTTCTTTGTTGGTCATTGTTTCTGGCTGTCAAAATGATGATAGTAATTCTGCAAGCCAACTAA
AACAGCCTGCCCCGGCAGTA

Downstream 100 bases:

>100_bases
CCAGTGTTTAGTAATTTTTTTATCGAGCGTCCCCGCTTCGCTTTCGTCATTTCTATTGTCATTGTACTTGCTGGCCTGAT
ATCCCTGTTAAAACTGCCTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MVTPVIQEKVAKIHELVGRTAALRTVNLVARVQGFLERRNFIEGDSVKEGDILYVIEQAPYQIEVKAAQAKVAEAKATLQ
NAKSYLQRLKTVRQGGVSQMDLDKASSDFLSAQALLTSAQAMLDRAKLNLSYTEIRSPINGRIGRNSISTGNLVSPNSGI
LATIVLIDPLYVLFTVSEVDFLTELQNQLEKGESTIFVPKIQLANGTIYPFEGKSDFISPIVDEKTGTITIRAIFPNPDT
KELLGQTNTPQSRRLLMPGQFVKVLVRRDDVEEEHVIPQSAMQEDQSGKYVLVVDADNRVQKRSVTVGDKIGVNWIIKRG
LQVGELVISEGAQKVRPGITVQTTIAVPSQPIEK

Sequences:

>Translated_354_residues
MVTPVIQEKVAKIHELVGRTAALRTVNLVARVQGFLERRNFIEGDSVKEGDILYVIEQAPYQIEVKAAQAKVAEAKATLQ
NAKSYLQRLKTVRQGGVSQMDLDKASSDFLSAQALLTSAQAMLDRAKLNLSYTEIRSPINGRIGRNSISTGNLVSPNSGI
LATIVLIDPLYVLFTVSEVDFLTELQNQLEKGESTIFVPKIQLANGTIYPFEGKSDFISPIVDEKTGTITIRAIFPNPDT
KELLGQTNTPQSRRLLMPGQFVKVLVRRDDVEEEHVIPQSAMQEDQSGKYVLVVDADNRVQKRSVTVGDKIGVNWIIKRG
LQVGELVISEGAQKVRPGITVQTTIAVPSQPIEK
>Mature_354_residues
MVTPVIQEKVAKIHELVGRTAALRTVNLVARVQGFLERRNFIEGDSVKEGDILYVIEQAPYQIEVKAAQAKVAEAKATLQ
NAKSYLQRLKTVRQGGVSQMDLDKASSDFLSAQALLTSAQAMLDRAKLNLSYTEIRSPINGRIGRNSISTGNLVSPNSGI
LATIVLIDPLYVLFTVSEVDFLTELQNQLEKGESTIFVPKIQLANGTIYPFEGKSDFISPIVDEKTGTITIRAIFPNPDT
KELLGQTNTPQSRRLLMPGQFVKVLVRRDDVEEEHVIPQSAMQEDQSGKYVLVVDADNRVQKRSVTVGDKIGVNWIIKRG
LQVGELVISEGAQKVRPGITVQTTIAVPSQPIEK

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=336, Percent_Identity=35.7142857142857, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789665, Length=347, Percent_Identity=34.2939481268012, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1786668, Length=334, Percent_Identity=35.3293413173653, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI87082036, Length=347, Percent_Identity=28.5302593659942, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87081796, Length=345, Percent_Identity=28.695652173913, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081951, Length=139, Percent_Identity=29.4964028776978, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789637, Length=147, Percent_Identity=30.6122448979592, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38961; Mature: 38961

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTPVIQEKVAKIHELVGRTAALRTVNLVARVQGFLERRNFIEGDSVKEGDILYVIEQAP
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
YQIEVKAAQAKVAEAKATLQNAKSYLQRLKTVRQGGVSQMDLDKASSDFLSAQALLTSAQ
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AMLDRAKLNLSYTEIRSPINGRIGRNSISTGNLVSPNSGILATIVLIDPLYVLFTVSEVD
HHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEECCCCCCEEHHHHHCCHHHHHHHHHHH
FLTELQNQLEKGESTIFVPKIQLANGTIYPFEGKSDFISPIVDEKTGTITIRAIFPNPDT
HHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCHHHCHHHCCCCCEEEEEEECCCCCH
KELLGQTNTPQSRRLLMPGQFVKVLVRRDDVEEEHVIPQSAMQEDQSGKYVLVVDADNRV
HHHCCCCCCCCCCEEECCHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCEEEEEECCCCE
QKRSVTVGDKIGVNWIIKRGLQVGELVISEGAQKVRPGITVQTTIAVPSQPIEK
ECCEECCCCCCCHHHHHHCCCHHHHHHHHCCHHHHCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MVTPVIQEKVAKIHELVGRTAALRTVNLVARVQGFLERRNFIEGDSVKEGDILYVIEQAP
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
YQIEVKAAQAKVAEAKATLQNAKSYLQRLKTVRQGGVSQMDLDKASSDFLSAQALLTSAQ
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AMLDRAKLNLSYTEIRSPINGRIGRNSISTGNLVSPNSGILATIVLIDPLYVLFTVSEVD
HHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEECCCCCCEEHHHHHCCHHHHHHHHHHH
FLTELQNQLEKGESTIFVPKIQLANGTIYPFEGKSDFISPIVDEKTGTITIRAIFPNPDT
HHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCHHHCHHHCCCCCEEEEEEECCCCCH
KELLGQTNTPQSRRLLMPGQFVKVLVRRDDVEEEHVIPQSAMQEDQSGKYVLVVDADNRV
HHHCCCCCCCCCCEEECCHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCEEEEEECCCCE
QKRSVTVGDKIGVNWIIKRGLQVGELVISEGAQKVRPGITVQTTIAVPSQPIEK
ECCEECCCCCCCHHHHHHCCCHHHHHHHHCCHHHHCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]