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The map label for this gene is hsdM [H]

Identifier: 114331916

GI number: 114331916

Start: 2055692

End: 2056042

Strand: Direct

Name: hsdM [H]

Synonym: Neut_1942

Alternate gene names: 114331916

Gene position: 2055692-2056042 (Clockwise)

Preceding gene: 114331914

Following gene: 114331917

Centisome position: 77.25

GC content: 50.14

Gene sequence:

>351_bases
ATGCTGTCCAAACACAAAACTGACACCACCACCCAGTTTATGGATGCCAACGGCCTGTTTAAAAAAGAAACCAATAACAA
TACCCTCACCGACGCGCACATCGAACAGATCATGCAGGTATTTGGCAGCAAAGCGAATGTAGAGCATGTTGCCCAGTCGG
TACCGTTTGAGAAGGTTGCCGACGACGACTATAACCTGCCAGTATGCAGCTACGTTGACGCCAAAGACAACCGCGAAGTG
GTGGGCATTGCCAAGCTCAATGCAGAGCTGAAAACCACCGTTGCCAGAATCGGCCAACTGCGCCAAGATATTGACGCGAT
AGTTTCAGAAATAGAGGGGGCTGACGCATGA

Upstream 100 bases:

>100_bases
GTTGGTAGCAAAACCACGAATTAATCAGCATCAAATGATTTAACAGCATCCGAATATACAATCAGTGTAATACATGCTCT
TACCCTGATTGTATATTCGG

Downstream 100 bases:

>100_bases
TCCTGGAAAACAAGCTCAATATCACCGATCAAATCGAATTGGCCAAAGCCGAAGAAAAGATCAGCAAACAAAAAGCCAGG
CAACTGTTTGATTCTGGCGA

Product: type I site-specific deoxyribonuclease HsdM

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MLSKHKTDTTTQFMDANGLFKKETNNNTLTDAHIEQIMQVFGSKANVEHVAQSVPFEKVADDDYNLPVCSYVDAKDNREV
VGIAKLNAELKTTVARIGQLRQDIDAIVSEIEGADA

Sequences:

>Translated_116_residues
MLSKHKTDTTTQFMDANGLFKKETNNNTLTDAHIEQIMQVFGSKANVEHVAQSVPFEKVADDDYNLPVCSYVDAKDNREV
VGIAKLNAELKTTVARIGQLRQDIDAIVSEIEGADA
>Mature_116_residues
MLSKHKTDTTTQFMDANGLFKKETNNNTLTDAHIEQIMQVFGSKANVEHVAQSVPFEKVADDDYNLPVCSYVDAKDNREV
VGIAKLNAELKTTVARIGQLRQDIDAIVSEIEGADA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 12796; Mature: 12796

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKHKTDTTTQFMDANGLFKKETNNNTLTDAHIEQIMQVFGSKANVEHVAQSVPFEKVA
CCCCCCCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHC
DDDYNLPVCSYVDAKDNREVVGIAKLNAELKTTVARIGQLRQDIDAIVSEIEGADA
CCCCCCCEEEECCCCCCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSKHKTDTTTQFMDANGLFKKETNNNTLTDAHIEQIMQVFGSKANVEHVAQSVPFEKVA
CCCCCCCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHC
DDDYNLPVCSYVDAKDNREVVGIAKLNAELKTTVARIGQLRQDIDAIVSEIEGADA
CCCCCCCEEEECCCCCCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]