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The map label for this gene is hisF

Identifier: 114331884

GI number: 114331884

Start: 2023312

End: 2024088

Strand: Direct

Name: hisF

Synonym: Neut_1909

Alternate gene names: 114331884

Gene position: 2023312-2024088 (Clockwise)

Preceding gene: 114331883

Following gene: 114331885

Centisome position: 76.03

GC content: 50.84

Gene sequence:

>777_bases
ATGGGATTAGCCAAACGTATTATTCCCTGCCTTGATATTAAGGACGGGCGCGTCGTCAAAGGTGTCAATTTTGTCTCTCT
GCGGGATGCAGGAGATCCGGTTGAAATTGCTCGCAGCTATAATGAGCAGGGAGCGGATGAACTCGTCTTTCTCGATATTA
CTGCAAGCGTGGAGAATCGCGATTTAATCCTGCATATTGTGGAAAAGGTCGCCTCACAGGTTTTCATCCCACTAACGGTG
GGAGGAGGCGTCTGCAAGGCCGAGGATGTGCGCCGGCTGCTGAACGCGGGGGCTGACAAGGTCAGCATCAACACTTCCGC
AGTATTGAATCCGGAACTGATCAAGGAATCAGCAGATCATTACGGTTCTCAGTGTATCGTAATCGCCATTGATGCCAGGC
AAACATCTGACGGAAACCCTGAATCTCCGCGCTGGGAAGTATTCACCCATGGCGGGCGCAAGCCAACCGGGCTTGATGCG
ATTGAATGGGCTCAAAAGATACAATCCTTGGGTGCCGGAGAAATTCTGCTGACCAGCATGGATCGTGATGGTACTCGCTC
CGGATTTGATCTTGTTCTGACACGCACAATCTCCGATGCAGTAGACTTACCCGTTATTGCCAGCGGTGGTGTAGGTAACC
TGGATCATCTGGTGGACGGTATTTTGCAAGGCCATGCTGATGCAGTGCTGGCAGCCAGTATTTTTCATTATGGCGAATTC
AGTATCCGTCAGGCCAAGGAATATCTGTTATCACACGGCATAGAAGTCCGCCTGTAA

Upstream 100 bases:

>100_bases
CAGCAAAGTGATTGACATCACTCACTCACAGAAACTCACTGGCTTTGTGCTTTTTATCCCCTTCTTTTTTTATCTAATTT
ATTTCTGATAACTTTGACCA

Downstream 100 bases:

>100_bases
GAAAGTGGAACACGGTCTTTACTTCTGAGTCGCCACAATTACCATGGAGGCCGTTTTTCCTACGGTATTCCCACATTTTG
CAAGTATTCCTGAAACTGTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGLAKRIIPCLDIKDGRVVKGVNFVSLRDAGDPVEIARSYNEQGADELVFLDITASVENRDLILHIVEKVASQVFIPLTV
GGGVCKAEDVRRLLNAGADKVSINTSAVLNPELIKESADHYGSQCIVIAIDARQTSDGNPESPRWEVFTHGGRKPTGLDA
IEWAQKIQSLGAGEILLTSMDRDGTRSGFDLVLTRTISDAVDLPVIASGGVGNLDHLVDGILQGHADAVLAASIFHYGEF
SIRQAKEYLLSHGIEVRL

Sequences:

>Translated_258_residues
MGLAKRIIPCLDIKDGRVVKGVNFVSLRDAGDPVEIARSYNEQGADELVFLDITASVENRDLILHIVEKVASQVFIPLTV
GGGVCKAEDVRRLLNAGADKVSINTSAVLNPELIKESADHYGSQCIVIAIDARQTSDGNPESPRWEVFTHGGRKPTGLDA
IEWAQKIQSLGAGEILLTSMDRDGTRSGFDLVLTRTISDAVDLPVIASGGVGNLDHLVDGILQGHADAVLAASIFHYGEF
SIRQAKEYLLSHGIEVRL
>Mature_257_residues
GLAKRIIPCLDIKDGRVVKGVNFVSLRDAGDPVEIARSYNEQGADELVFLDITASVENRDLILHIVEKVASQVFIPLTVG
GGVCKAEDVRRLLNAGADKVSINTSAVLNPELIKESADHYGSQCIVIAIDARQTSDGNPESPRWEVFTHGGRKPTGLDAI
EWAQKIQSLGAGEILLTSMDRDGTRSGFDLVLTRTISDAVDLPVIASGGVGNLDHLVDGILQGHADAVLAASIFHYGEFS
IRQAKEYLLSHGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=42.3076923076923, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082028, Length=246, Percent_Identity=28.8617886178862, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=319, Percent_Identity=35.7366771159875, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_NITEC (Q0AEU1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748106.1
- ProteinModelPortal:   Q0AEU1
- SMR:   Q0AEU1
- STRING:   Q0AEU1
- GeneID:   4273693
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1909
- NMPDR:   fig|335283.3.peg.1033
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q0AEU1
- ProtClustDB:   PRK02083
- BioCyc:   NEUT335283:NEUT_1909-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 2.4.2.-

Molecular weight: Translated: 27749; Mature: 27618

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAKRIIPCLDIKDGRVVKGVNFVSLRDAGDPVEIARSYNEQGADELVFLDITASVENR
CCCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILHIVEKVASQVFIPLTVGGGVCKAEDVRRLLNAGADKVSINTSAVLNPELIKESADH
HHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCEECCHHHHHHHHHH
YGSQCIVIAIDARQTSDGNPESPRWEVFTHGGRKPTGLDAIEWAQKIQSLGAGEILLTSM
CCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEC
DRDGTRSGFDLVLTRTISDAVDLPVIASGGVGNLDHLVDGILQGHADAVLAASIFHYGEF
CCCCCCCCCEEEEEEHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCH
SIRQAKEYLLSHGIEVRL
HHHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
GLAKRIIPCLDIKDGRVVKGVNFVSLRDAGDPVEIARSYNEQGADELVFLDITASVENR
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILHIVEKVASQVFIPLTVGGGVCKAEDVRRLLNAGADKVSINTSAVLNPELIKESADH
HHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCEECCHHHHHHHHHH
YGSQCIVIAIDARQTSDGNPESPRWEVFTHGGRKPTGLDAIEWAQKIQSLGAGEILLTSM
CCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEC
DRDGTRSGFDLVLTRTISDAVDLPVIASGGVGNLDHLVDGILQGHADAVLAASIFHYGEF
CCCCCCCCCEEEEEEHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCH
SIRQAKEYLLSHGIEVRL
HHHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA