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The map label for this gene is hisH [H]

Identifier: 114331882

GI number: 114331882

Start: 2021753

End: 2022391

Strand: Direct

Name: hisH [H]

Synonym: Neut_1907

Alternate gene names: 114331882

Gene position: 2021753-2022391 (Clockwise)

Preceding gene: 114331881

Following gene: 114331883

Centisome position: 75.98

GC content: 48.2

Gene sequence:

>639_bases
ATGAACTCGATTGCCGTTGTAGATTATGGGATGGGCAACCTGAGATCGGTATCCAAAGCACTGGAATATGTCGATTCATC
TGCGGTTGTCACGGTTACCAATGATCCGGAGGTTATCCGATCTGCCTCACGCGTTGTTGTTCCGGGCCAGGGTGCGATGC
CTCATTGCATGGAGGCTCTGGATGATCAGGGTTTGCGTGAAGTAGTCGTCGAAGCAGCTCAAAATAAACCTTTTCTCGGC
ATTTGCCTCGGACTGCAAATGTTGTTCAAGGAAAGTGAGGAAGGCGATACCTCAGCACTGGGTATTTTGCCAGGCAGTGT
GAAAAAATTAGACGTGCGTGAGACCGCCGGTAACAACACCCCAAAACTCAAGATTCCTCACATGGGCTGGAACCAGGTTC
ACCAGGTTATGGCGCATCCACTGTGGGATAGAATTGATACCGGCACACGCTTTTACTTTGTTCACAGCTATTACGTAGCT
ACAGATGAACCAGGAATCGTGGCGGGTAGTACCAACTATCCAACCCCATTCACATGTGCAGTTGCCAGAGACAATATTTT
TGCCACCCAGTTTCACCCGGAGAAAAGTCATTCCGCAGGATTGGCATTATTGAGTAATTTCCTAAAATGGATACCATAA

Upstream 100 bases:

>100_bases
TGCGTATGGCTGTAACAATTGATCCACGCTGCGATGACCTTATTCCATCCACCAAAGGCGTGCTGTAGGTAAAAAAACGC
TCAGAACATCTGATACGAAC

Downstream 100 bases:

>100_bases
CAACATTCCAGCATTATCCTATCCAGCATTTCCATTGTATTATCAATTTTTTTCAAGTCTGATCATTAACCCCGATAACT
TCGTATGCTGATTATTCCTG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNSIAVVDYGMGNLRSVSKALEYVDSSAVVTVTNDPEVIRSASRVVVPGQGAMPHCMEALDDQGLREVVVEAAQNKPFLG
ICLGLQMLFKESEEGDTSALGILPGSVKKLDVRETAGNNTPKLKIPHMGWNQVHQVMAHPLWDRIDTGTRFYFVHSYYVA
TDEPGIVAGSTNYPTPFTCAVARDNIFATQFHPEKSHSAGLALLSNFLKWIP

Sequences:

>Translated_212_residues
MNSIAVVDYGMGNLRSVSKALEYVDSSAVVTVTNDPEVIRSASRVVVPGQGAMPHCMEALDDQGLREVVVEAAQNKPFLG
ICLGLQMLFKESEEGDTSALGILPGSVKKLDVRETAGNNTPKLKIPHMGWNQVHQVMAHPLWDRIDTGTRFYFVHSYYVA
TDEPGIVAGSTNYPTPFTCAVARDNIFATQFHPEKSHSAGLALLSNFLKWIP
>Mature_212_residues
MNSIAVVDYGMGNLRSVSKALEYVDSSAVVTVTNDPEVIRSASRVVVPGQGAMPHCMEALDDQGLREVVVEAAQNKPFLG
ICLGLQMLFKESEEGDTSALGILPGSVKKLDVRETAGNNTPKLKIPHMGWNQVHQVMAHPLWDRIDTGTRFYFVHSYYVA
TDEPGIVAGSTNYPTPFTCAVARDNIFATQFHPEKSHSAGLALLSNFLKWIP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=37.0192307692308, Blast_Score=130, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=220, Percent_Identity=31.3636363636364, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23146; Mature: 23146

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIAVVDYGMGNLRSVSKALEYVDSSAVVTVTNDPEVIRSASRVVVPGQGAMPHCMEAL
CCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHCCCEEEECCCCCCHHHHHHH
DDQGLREVVVEAAQNKPFLGICLGLQMLFKESEEGDTSALGILPGSVKKLDVRETAGNNT
CCCCHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEHHHCCCCC
PKLKIPHMGWNQVHQVMAHPLWDRIDTGTRFYFVHSYYVATDEPGIVAGSTNYPTPFTCA
CEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEECCCCCCCCEEEE
VARDNIFATQFHPEKSHSAGLALLSNFLKWIP
EECCCEEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSIAVVDYGMGNLRSVSKALEYVDSSAVVTVTNDPEVIRSASRVVVPGQGAMPHCMEAL
CCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHCCCEEEECCCCCCHHHHHHH
DDQGLREVVVEAAQNKPFLGICLGLQMLFKESEEGDTSALGILPGSVKKLDVRETAGNNT
CCCCHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEHHHCCCCC
PKLKIPHMGWNQVHQVMAHPLWDRIDTGTRFYFVHSYYVATDEPGIVAGSTNYPTPFTCA
CEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEECCCCCCCCEEEE
VARDNIFATQFHPEKSHSAGLALLSNFLKWIP
EECCCEEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]