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The map label for this gene is metK

Identifier: 114331868

GI number: 114331868

Start: 2008832

End: 2009995

Strand: Reverse

Name: metK

Synonym: Neut_1893

Alternate gene names: 114331868

Gene position: 2009995-2008832 (Counterclockwise)

Preceding gene: 114331872

Following gene: 114331867

Centisome position: 75.53

GC content: 49.57

Gene sequence:

>1164_bases
ATGAGTAACTATCTTTTTACGTCTGAATCCGTATCTGAAGGCCACCCTGATAAGGTGGCCGACCAGATATCGGATGCAAT
CCTTGATGCAATTCTGCAACAGGATCCTCATGCGCGTGTAGCCTGTGAAACCATGTGCAGTACAGGGCTGATCGTTTTGT
CAGGTGAAATAACGACCCATGCTACCATTGATTACAACGTCATCCCACGAGATACGGTGCGTGAAATCGGCTATACCAGT
TCCGAGATTGGCTTTGATGCAAGCACCTGCGCGGTGCTGACCGCTTTTAACAAACAGTCTCCCGATATTGCTCTGGGGGT
GAATCGCAGCAAGGAAGAGGAGATGGATCAGGGGGCGGGAGATCAGGGACTGATGTTCGGTTATGCCTGTGATGAAACGC
CGCAACTAATGCCGCTGCCGATTTACTATGCACATCGTTTGGTTGAACAGCAGGCAAAATTGAGAAAAAGCGGCCAGCTG
TCATGGCTGCGTCCGGATGCCAAATCCCAGGTTTCAGTACGCTATATTGACGGGAAACCGAAAAACATAGAAACAGTCGT
TATTTCAACCCAGCATGATCCGGATATTTCCAATGGCGATTTGTTTGAAGGAGTTGTCGAGGAAATCATCAAACCTGTAT
TGCCTGCAGAAATGCTGAGTAGTGATATTCGGTATCTGGTCAATCCTACCGGTCGTTTCGTGGTGGGCGGGCCGATGGGC
GATTGTGGACTAACCGGGCGCAAGATCATCGTGGATACTTACGGTGGCACAGCCCATCATGGTGGCGGTGCTTTTTCCGG
TAAGGATCCTTCCAAGGTGGATCGCTCAGCAGCTTATGCTGCCCGTTATGTCGCCAAAAATATTGTAGCTGCCGGCCTGG
CACGCAAATGTGAAGTTCAGGTGGCTTATGCAATTGGCGTGGCTAAGCCGGTTTCATTAATGTTGGAAACTTTTGGTACC
GGTAAAGTTTCAGATGAAAAACTCGCCGAGCTGATCACCCGGAATTTTGATCTGCGCCCCAGAGCGATCATTCATGAACT
GAATCTGCTGCGTCCGATTTATAGCAAAACGGCTGCGTATGGCCATTTTGGTCGGGAAGAACCCAGTTTCACCTGGGAGA
AAACCGACATTGCAGAGCAGCTTATAGCGGATGCAGGTATTTGA

Upstream 100 bases:

>100_bases
TAATAAATAACTGCAGTCTTTGGCCTCAAATCCGGAATTGGTTTTACTCTTGCCTGAGTATTATCTCATTCATATCCAAA
TTTAATTAACAAAACAAACT

Downstream 100 bases:

>100_bases
ATATTTTTCATCAACATCATTCAGCGTGAATGATGTTCCATTTGTACCGAGGAGCGCTGCAACGGTCTATCTCGTGAGGC
TCGGACAAATGAACAACCCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSNYLFTSESVSEGHPDKVADQISDAILDAILQQDPHARVACETMCSTGLIVLSGEITTHATIDYNVIPRDTVREIGYTS
SEIGFDASTCAVLTAFNKQSPDIALGVNRSKEEEMDQGAGDQGLMFGYACDETPQLMPLPIYYAHRLVEQQAKLRKSGQL
SWLRPDAKSQVSVRYIDGKPKNIETVVISTQHDPDISNGDLFEGVVEEIIKPVLPAEMLSSDIRYLVNPTGRFVVGGPMG
DCGLTGRKIIVDTYGGTAHHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARKCEVQVAYAIGVAKPVSLMLETFGT
GKVSDEKLAELITRNFDLRPRAIIHELNLLRPIYSKTAAYGHFGREEPSFTWEKTDIAEQLIADAGI

Sequences:

>Translated_387_residues
MSNYLFTSESVSEGHPDKVADQISDAILDAILQQDPHARVACETMCSTGLIVLSGEITTHATIDYNVIPRDTVREIGYTS
SEIGFDASTCAVLTAFNKQSPDIALGVNRSKEEEMDQGAGDQGLMFGYACDETPQLMPLPIYYAHRLVEQQAKLRKSGQL
SWLRPDAKSQVSVRYIDGKPKNIETVVISTQHDPDISNGDLFEGVVEEIIKPVLPAEMLSSDIRYLVNPTGRFVVGGPMG
DCGLTGRKIIVDTYGGTAHHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARKCEVQVAYAIGVAKPVSLMLETFGT
GKVSDEKLAELITRNFDLRPRAIIHELNLLRPIYSKTAAYGHFGREEPSFTWEKTDIAEQLIADAGI
>Mature_386_residues
SNYLFTSESVSEGHPDKVADQISDAILDAILQQDPHARVACETMCSTGLIVLSGEITTHATIDYNVIPRDTVREIGYTSS
EIGFDASTCAVLTAFNKQSPDIALGVNRSKEEEMDQGAGDQGLMFGYACDETPQLMPLPIYYAHRLVEQQAKLRKSGQLS
WLRPDAKSQVSVRYIDGKPKNIETVVISTQHDPDISNGDLFEGVVEEIIKPVLPAEMLSSDIRYLVNPTGRFVVGGPMGD
CGLTGRKIIVDTYGGTAHHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARKCEVQVAYAIGVAKPVSLMLETFGTG
KVSDEKLAELITRNFDLRPRAIIHELNLLRPIYSKTAAYGHFGREEPSFTWEKTDIAEQLIADAGI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=57.4866310160428, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=57.3333333333333, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=67.7002583979328, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=58.2446808510638, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=57.4468085106383, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=377, Percent_Identity=57.8249336870027, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=377, Percent_Identity=56.2334217506631, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=375, Percent_Identity=52.2666666666667, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=55.6231003039514, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=329, Percent_Identity=55.3191489361702, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=54.9668874172185, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=63.4146341463415, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=82.051282051282, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=376, Percent_Identity=57.4468085106383, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=57.4468085106383, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=55.7029177718833, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=55.7029177718833, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=55.7029177718833, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=55.7029177718833, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=55.7029177718833, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=55.7029177718833, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=377, Percent_Identity=55.7029177718833, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=377, Percent_Identity=55.7029177718833, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=53.7234042553192, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=61.3445378151261, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_NITEC (Q0AEV7)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748090.1
- ProteinModelPortal:   Q0AEV7
- SMR:   Q0AEV7
- STRING:   Q0AEV7
- GeneID:   4274139
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1893
- NMPDR:   fig|335283.3.peg.1017
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q0AEV7
- ProtClustDB:   PRK05250
- BioCyc:   NEUT335283:NEUT_1893-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42057; Mature: 41926

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYLFTSESVSEGHPDKVADQISDAILDAILQQDPHARVACETMCSTGLIVLSGEITTH
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCEEE
ATIDYNVIPRDTVREIGYTSSEIGFDASTCAVLTAFNKQSPDIALGVNRSKEEEMDQGAG
EEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHEEECCCCCCCEEEECCCCHHHHHHCCCC
DQGLMFGYACDETPQLMPLPIYYAHRLVEQQAKLRKSGQLSWLRPDAKSQVSVRYIDGKP
CCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCC
KNIETVVISTQHDPDISNGDLFEGVVEEIIKPVLPAEMLSSDIRYLVNPTGRFVVGGPMG
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGTAHHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARKCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VAYAIGVAKPVSLMLETFGTGKVSDEKLAELITRNFDLRPRAIIHELNLLRPIYSKTAAY
EEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPSFTWEKTDIAEQLIADAGI
CCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNYLFTSESVSEGHPDKVADQISDAILDAILQQDPHARVACETMCSTGLIVLSGEITTH
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCEEE
ATIDYNVIPRDTVREIGYTSSEIGFDASTCAVLTAFNKQSPDIALGVNRSKEEEMDQGAG
EEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHEEECCCCCCCEEEECCCCHHHHHHCCCC
DQGLMFGYACDETPQLMPLPIYYAHRLVEQQAKLRKSGQLSWLRPDAKSQVSVRYIDGKP
CCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCC
KNIETVVISTQHDPDISNGDLFEGVVEEIIKPVLPAEMLSSDIRYLVNPTGRFVVGGPMG
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGTAHHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARKCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VAYAIGVAKPVSLMLETFGTGKVSDEKLAELITRNFDLRPRAIIHELNLLRPIYSKTAAY
EEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPSFTWEKTDIAEQLIADAGI
CCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA