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The map label for this gene is glyS

Identifier: 114331843

GI number: 114331843

Start: 1979508

End: 1981655

Strand: Reverse

Name: glyS

Synonym: Neut_1868

Alternate gene names: 114331843

Gene position: 1981655-1979508 (Counterclockwise)

Preceding gene: 114331844

Following gene: 114331842

Centisome position: 74.47

GC content: 52.75

Gene sequence:

>2148_bases
ATGACGACTGAAAGTCTGCTAATTGAGTTGCTGACCGAGGAACTGCCTCCCAAATCACTCGTAAAACTGGGCAATGCATT
TGCCACATTAATTGCTGATAGTCTAAAACAACAGTATTTGACCACGCCGGACACGGTGTCGACTGTTTTTGCCTCACCCC
GTCGGCTGGCGGTGCATCTGACTGCTATTTCTGTCCAGGCACCAGATCAGATCGTCACCCTCAAACTGATGCCGGTGGCA
GTCGGGCTGGATGCGCAAGGACAGCCCACTCCGGCGCTGCATAAAAAACTGGCTGCATTGGGAGTGGAAAATGTTGCTGT
CTCCGCGCTCAAGCGGGTACAGGAAGGCAAGGCAGAGATGTTGTTCCTGGAACAGAGCGTTACCGGAATCCTGCTGGCGG
CAGGGTTGCAAAAAGCCGTGGATGAGGCCATTCGCCAGTTGCCGGTTGCCAAGATGATGACCTATCAGCTGGATGATGGC
TGGGAGAATGTGCACTTTATCCGTCCGGCACATGGCCTGATTGCCCTGCATGGACAGCAGATTGTGCCGATCAGCGCTTT
AGGGTTCGCTGCTGGTAACACCACCCGGGGGCATCGTTTTGAAGCAAAACAGGCGGAATTGGTGATTGATCATGCAGATC
ACTATGAATACATCCTGGAAACAGAAGGAACCGTTATTCCGGGATTTGATCGTCGTCGCAGCCGCATCAGGGAAGGGTTG
GAGACTGCCACGCGTGCAGCACAATTGCAGTGTATCGAGGATGCAGCTTTGCTGGATGAGGTGACGGCGCTGGTAGAGCG
CCCCAATGTTCTGATGGGAACATTTTCAGCAGATTTTCTGGAAGTGCCGCAGGAATGTCTGATCTCCACCATGAAAATTA
ACCAGAAATATTTCCCCCTGCTGGATGCTGATGGAAAACTGACCAATCGGTTTTTGATTGTTTCCAATATCACGCCGGCT
GATCCAGGGCAGATCATTGCGGGTAACGAGCGCGTCATCCGTTCCAGACTGGCGGATGCAAAATTTTTCTTTGATCACGA
TCGCAAACGAACATTGGCTTCACGTCTGCCCGATCTGGACAAGGTGATCTACCATCATCAACTCGGTTCACAGGGCGAAC
GTACCCGATATGTACGTGCCTTGGCGCAAATTATCGGACAGTTGCTGGACAACGGCGACTTGCCTGCACAGGCGAATCAG
GCTGCGATGCTGGCCAAGGCCGATCTACTGACTGATATGGTTGGAGAATTTCCCGAGCTGCAAGGCATTATGGGGCGTTA
TTACGCTCAGCGCGAAGGTGTGGATGAAACCATTGCCTTCGCTATTGAGGATCACTACAAACCGCGCTTTGCCGGGGATG
CATTACCACGCAGCATGACGGGTATCTGTGTGGCACTGGCTGACAAACTGGAAACGCTGATCAGCCTGTTCAGTATTGGC
CAATTCCCGACAGGGGACAAGGATCCTTATGCCCTGCGGCGACATGCACTGGGTGTAATCCGCATCCTTATCGAGAAAGA
TCTACCGATCGGACTGGATGTGCTGATTTCACGCGCAGCTGGTGTACTGCGGGATGAAATCAGCGGGGAACAAGGCCCAG
AATCAAGCCATGCCCGTCCGGTAACACCACAACTGACAGAACAGTTGCAGGATTTCTTTTACGATCGTCTTGCCAGCAGC
CTGCGTGATCAAGGCTACACTGTTCAGGAGGTAGAATCTGTCCTGAATCTGCGGCCCTCCCTGTTGTGTGAAATCCCCAG
GAGACTAGCTGCTGTGCGGGCATTTACTGCCTTGCCTGAAGCAGCCAGTCTGGCAGCTGCCAACAAGCGGGTCAGCAACA
TCCTCAAAAAATCGGAATTTGATGCCACGGTCAGCATTGATAAGGCCTGCTTGCAGGCGCCGGCTGAAATTGCTCTGTAT
CGTGCGTTGTCTGAAATAGAATCCGATGCCCGGCACGCCTTCCAGAAGGGTGATTATGTTACTGCCCTGCAAATGCTCGC
TGCCTTGAAAGCGCCGATTGATGCTTTCTTTGACCAGGTGATGGTGAATGATGAAAATGAAGTGTTGCGGCGTAACCGTC
TGGCCCTGTTGGCAGCCTTACAGGCAACGATGAATCGTGTAGCAGATATTTCCCGGCTAGCTGCCTGA

Upstream 100 bases:

>100_bases
TGGCACAAGCCTACTACGACAGTCGCTTGCGTCTGCAGCCGCCATTTCCATTGGCACCACGTGAATGGGTTGCTCAAACC
CTGGATCAGGTAGGATCAGC

Downstream 100 bases:

>100_bases
ATGACTTGAGAATGCATAGTAAATAAATGAAGTTGATTATTCTCGACCAGAATGGTGTCATTAATCAGCGGGGTGATACG
CTTATCAAGACGCCTGATGA

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MTTESLLIELLTEELPPKSLVKLGNAFATLIADSLKQQYLTTPDTVSTVFASPRRLAVHLTAISVQAPDQIVTLKLMPVA
VGLDAQGQPTPALHKKLAALGVENVAVSALKRVQEGKAEMLFLEQSVTGILLAAGLQKAVDEAIRQLPVAKMMTYQLDDG
WENVHFIRPAHGLIALHGQQIVPISALGFAAGNTTRGHRFEAKQAELVIDHADHYEYILETEGTVIPGFDRRRSRIREGL
ETATRAAQLQCIEDAALLDEVTALVERPNVLMGTFSADFLEVPQECLISTMKINQKYFPLLDADGKLTNRFLIVSNITPA
DPGQIIAGNERVIRSRLADAKFFFDHDRKRTLASRLPDLDKVIYHHQLGSQGERTRYVRALAQIIGQLLDNGDLPAQANQ
AAMLAKADLLTDMVGEFPELQGIMGRYYAQREGVDETIAFAIEDHYKPRFAGDALPRSMTGICVALADKLETLISLFSIG
QFPTGDKDPYALRRHALGVIRILIEKDLPIGLDVLISRAAGVLRDEISGEQGPESSHARPVTPQLTEQLQDFFYDRLASS
LRDQGYTVQEVESVLNLRPSLLCEIPRRLAAVRAFTALPEAASLAAANKRVSNILKKSEFDATVSIDKACLQAPAEIALY
RALSEIESDARHAFQKGDYVTALQMLAALKAPIDAFFDQVMVNDENEVLRRNRLALLAALQATMNRVADISRLAA

Sequences:

>Translated_715_residues
MTTESLLIELLTEELPPKSLVKLGNAFATLIADSLKQQYLTTPDTVSTVFASPRRLAVHLTAISVQAPDQIVTLKLMPVA
VGLDAQGQPTPALHKKLAALGVENVAVSALKRVQEGKAEMLFLEQSVTGILLAAGLQKAVDEAIRQLPVAKMMTYQLDDG
WENVHFIRPAHGLIALHGQQIVPISALGFAAGNTTRGHRFEAKQAELVIDHADHYEYILETEGTVIPGFDRRRSRIREGL
ETATRAAQLQCIEDAALLDEVTALVERPNVLMGTFSADFLEVPQECLISTMKINQKYFPLLDADGKLTNRFLIVSNITPA
DPGQIIAGNERVIRSRLADAKFFFDHDRKRTLASRLPDLDKVIYHHQLGSQGERTRYVRALAQIIGQLLDNGDLPAQANQ
AAMLAKADLLTDMVGEFPELQGIMGRYYAQREGVDETIAFAIEDHYKPRFAGDALPRSMTGICVALADKLETLISLFSIG
QFPTGDKDPYALRRHALGVIRILIEKDLPIGLDVLISRAAGVLRDEISGEQGPESSHARPVTPQLTEQLQDFFYDRLASS
LRDQGYTVQEVESVLNLRPSLLCEIPRRLAAVRAFTALPEAASLAAANKRVSNILKKSEFDATVSIDKACLQAPAEIALY
RALSEIESDARHAFQKGDYVTALQMLAALKAPIDAFFDQVMVNDENEVLRRNRLALLAALQATMNRVADISRLAA
>Mature_714_residues
TTESLLIELLTEELPPKSLVKLGNAFATLIADSLKQQYLTTPDTVSTVFASPRRLAVHLTAISVQAPDQIVTLKLMPVAV
GLDAQGQPTPALHKKLAALGVENVAVSALKRVQEGKAEMLFLEQSVTGILLAAGLQKAVDEAIRQLPVAKMMTYQLDDGW
ENVHFIRPAHGLIALHGQQIVPISALGFAAGNTTRGHRFEAKQAELVIDHADHYEYILETEGTVIPGFDRRRSRIREGLE
TATRAAQLQCIEDAALLDEVTALVERPNVLMGTFSADFLEVPQECLISTMKINQKYFPLLDADGKLTNRFLIVSNITPAD
PGQIIAGNERVIRSRLADAKFFFDHDRKRTLASRLPDLDKVIYHHQLGSQGERTRYVRALAQIIGQLLDNGDLPAQANQA
AMLAKADLLTDMVGEFPELQGIMGRYYAQREGVDETIAFAIEDHYKPRFAGDALPRSMTGICVALADKLETLISLFSIGQ
FPTGDKDPYALRRHALGVIRILIEKDLPIGLDVLISRAAGVLRDEISGEQGPESSHARPVTPQLTEQLQDFFYDRLASSL
RDQGYTVQEVESVLNLRPSLLCEIPRRLAAVRAFTALPEAASLAAANKRVSNILKKSEFDATVSIDKACLQAPAEIALYR
ALSEIESDARHAFQKGDYVTALQMLAALKAPIDAFFDQVMVNDENEVLRRNRLALLAALQATMNRVADISRLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=713, Percent_Identity=39.4109396914446, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_NITEC (Q0AEY2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748065.1
- ProteinModelPortal:   Q0AEY2
- STRING:   Q0AEY2
- GeneID:   4272330
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1868
- NMPDR:   fig|335283.3.peg.2334
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q0AEY2
- ProtClustDB:   PRK01233
- BioCyc:   NEUT335283:NEUT_1868-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 78566; Mature: 78435

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTESLLIELLTEELPPKSLVKLGNAFATLIADSLKQQYLTTPDTVSTVFASPRRLAVHL
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEE
TAISVQAPDQIVTLKLMPVAVGLDAQGQPTPALHKKLAALGVENVAVSALKRVQEGKAEM
EEEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
LFLEQSVTGILLAAGLQKAVDEAIRQLPVAKMMTYQLDDGWENVHFIRPAHGLIALHGQQ
EEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCEEEECCCCCEEEECCCE
IVPISALGFAAGNTTRGHRFEAKQAELVIDHADHYEYILETEGTVIPGFDRRRSRIREGL
ECEEHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCEEEEECCCCEECCCHHHHHHHHHHH
ETATRAAQLQCIEDAALLDEVTALVERPNVLMGTFSADFLEVPQECLISTMKINQKYFPL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCHHHHHHHHHCCCCCCCE
LDADGKLTNRFLIVSNITPADPGQIIAGNERVIRSRLADAKFFFDHDRKRTLASRLPDLD
ECCCCCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
KVIYHHQLGSQGERTRYVRALAQIIGQLLDNGDLPAQANQAAMLAKADLLTDMVGEFPEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
QGIMGRYYAQREGVDETIAFAIEDHYKPRFAGDALPRSMTGICVALADKLETLISLFSIG
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
QFPTGDKDPYALRRHALGVIRILIEKDLPIGLDVLISRAAGVLRDEISGEQGPESSHARP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VTPQLTEQLQDFFYDRLASSLRDQGYTVQEVESVLNLRPSLLCEIPRRLAAVRAFTALPE
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCH
AASLAAANKRVSNILKKSEFDATVSIDKACLQAPAEIALYRALSEIESDARHAFQKGDYV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHH
TALQMLAALKAPIDAFFDQVMVNDENEVLRRNRLALLAALQATMNRVADISRLAA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTESLLIELLTEELPPKSLVKLGNAFATLIADSLKQQYLTTPDTVSTVFASPRRLAVHL
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEE
TAISVQAPDQIVTLKLMPVAVGLDAQGQPTPALHKKLAALGVENVAVSALKRVQEGKAEM
EEEEEECCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
LFLEQSVTGILLAAGLQKAVDEAIRQLPVAKMMTYQLDDGWENVHFIRPAHGLIALHGQQ
EEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCEEEECCCCCEEEECCCE
IVPISALGFAAGNTTRGHRFEAKQAELVIDHADHYEYILETEGTVIPGFDRRRSRIREGL
ECEEHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCEEEEECCCCEECCCHHHHHHHHHHH
ETATRAAQLQCIEDAALLDEVTALVERPNVLMGTFSADFLEVPQECLISTMKINQKYFPL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCHHHHHHHHHCCCCCCCE
LDADGKLTNRFLIVSNITPADPGQIIAGNERVIRSRLADAKFFFDHDRKRTLASRLPDLD
ECCCCCCCCEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
KVIYHHQLGSQGERTRYVRALAQIIGQLLDNGDLPAQANQAAMLAKADLLTDMVGEFPEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
QGIMGRYYAQREGVDETIAFAIEDHYKPRFAGDALPRSMTGICVALADKLETLISLFSIG
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
QFPTGDKDPYALRRHALGVIRILIEKDLPIGLDVLISRAAGVLRDEISGEQGPESSHARP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VTPQLTEQLQDFFYDRLASSLRDQGYTVQEVESVLNLRPSLLCEIPRRLAAVRAFTALPE
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCH
AASLAAANKRVSNILKKSEFDATVSIDKACLQAPAEIALYRALSEIESDARHAFQKGDYV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHH
TALQMLAALKAPIDAFFDQVMVNDENEVLRRNRLALLAALQATMNRVADISRLAA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA