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The map label for this gene is tuf1 [H]

Identifier: 114331779

GI number: 114331779

Start: 1911851

End: 1913041

Strand: Reverse

Name: tuf1 [H]

Synonym: Neut_1802

Alternate gene names: 114331779

Gene position: 1913041-1911851 (Counterclockwise)

Preceding gene: 114331780

Following gene: 114331778

Centisome position: 71.89

GC content: 48.28

Gene sequence:

>1191_bases
ATGGCGAAAAGTAAATTCGAGCGGGTGAAGCCGCATATAAATGTAGGGACGATAGGCCATGTGGATCATGGGAAAACGAC
GTTAACGGCGGCGATCACGACCATCCTGACCAGGAAATTTGGCGGAGAAGCCAAGTCATATGCTCAGATAGATTCGGCAC
CTGAGGAGCGTGCACGTGGGATTACTATTAACACATCCCATGTTGAATACGAAACAGATAAACGCCATTACGCGCACGTT
GATTGCCCGGGTCACGCCGACTATGTGAAGAATATGATCACAGGAGCGGCGCAGATGGATGGCGCTATTCTGGTGGTATC
AGCAGCTGATGGACCCATGCCGCAGACACGCGAGCATATTCTGCTGGCGCGGCAGGTGGGAGTGCCTTATATCATTGTCT
TCATGAATAAGGCTGACATGGTAGACGATGCTGAGTTACTTGAGCTCGTCGAAATGGAAATACGTGAACTGTTATCCAAG
TATGATTTTCCAGGGGATGATACACCTATTATTATTGGTTCAGCACTGAAAGCGCTGGAAGGGGATAAAGGTGACATCGG
TGAAGCAGCTATTCTGAAGCTGGCGGAAGTGCTGGATTCATACATCCCAGAGCCACAAAGAGCGATTGATGGGGCTTTTA
TTATGCCTGTTGAAGATGTGTTCTCAATCTCCGGGCGTGGAACTGTTGTTACAGGGCGTGTAGAGCGCGGTATAGTCAAG
GTTGGTGATGAGATAGAAATTGTGGGATTGCGACCTACAATCAAAACAACATGTACCGGTGTCGAGATGTTTCGGAAGTT
GCTGGATCAAGGGCAGGCCGGAGACAACGTTGGTATATTATTAAGAGGTACCAAGCGAGAAGAGGTGGAGCGTGGCCAGG
TTCTGGCTAAGCCGGGCAGTATACTGCCACACACCAAATTTTCTGCGGAGATATATGTGTTGAGCAAAGAAGAGGGTGGT
CGGCATACACCATTTTTTGCAGGATATCGCCCACAGTTTTATTTCAGAACAACTGATGTAACAGGCTCCATTGAACTGCC
GGCAGGCGTCGAAATGGTGATGCCAGGTGATAATATATCAGTTAATGTCAATTTGATTGCACCAATAGCAATGAGTGATG
GATTACGTTTTGCTATTCGTGAAGGTGGACGTACCGTCGGCGCTGGTGTGGTCGCCAAAGTAATCGAGTAA

Upstream 100 bases:

>100_bases
GGAGAGGTCGCCAGTTCAATCCTGGCCATGGGCACCAAGTTATGCTGCAGCCAGGTTATTAGATTACTGATACAAGCATT
AATAAAAGGAAACAGACGTA

Downstream 100 bases:

>100_bases
AATAAGCTGATAAATTTGTAGTCTGCAATTTTTTGTTTGGAAGATAGGCCAGTAGCTCAATTGGCAGAGCGTCGGTCTCC
AAAACCGAAGGTTGGGGGTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKSKFERVKPHINVGTIGHVDHGKTTLTAAITTILTRKFGGEAKSYAQIDSAPEERARGITINTSHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVFMNKADMVDDAELLELVEMEIRELLSK
YDFPGDDTPIIIGSALKALEGDKGDIGEAAILKLAEVLDSYIPEPQRAIDGAFIMPVEDVFSISGRGTVVTGRVERGIVK
VGDEIEIVGLRPTIKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREEVERGQVLAKPGSILPHTKFSAEIYVLSKEEGG
RHTPFFAGYRPQFYFRTTDVTGSIELPAGVEMVMPGDNISVNVNLIAPIAMSDGLRFAIREGGRTVGAGVVAKVIE

Sequences:

>Translated_396_residues
MAKSKFERVKPHINVGTIGHVDHGKTTLTAAITTILTRKFGGEAKSYAQIDSAPEERARGITINTSHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVFMNKADMVDDAELLELVEMEIRELLSK
YDFPGDDTPIIIGSALKALEGDKGDIGEAAILKLAEVLDSYIPEPQRAIDGAFIMPVEDVFSISGRGTVVTGRVERGIVK
VGDEIEIVGLRPTIKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREEVERGQVLAKPGSILPHTKFSAEIYVLSKEEGG
RHTPFFAGYRPQFYFRTTDVTGSIELPAGVEMVMPGDNISVNVNLIAPIAMSDGLRFAIREGGRTVGAGVVAKVIE
>Mature_395_residues
AKSKFERVKPHINVGTIGHVDHGKTTLTAAITTILTRKFGGEAKSYAQIDSAPEERARGITINTSHVEYETDKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVFMNKADMVDDAELLELVEMEIRELLSKY
DFPGDDTPIIIGSALKALEGDKGDIGEAAILKLAEVLDSYIPEPQRAIDGAFIMPVEDVFSISGRGTVVTGRVERGIVKV
GDEIEIVGLRPTIKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREEVERGQVLAKPGSILPHTKFSAEIYVLSKEEGGR
HTPFFAGYRPQFYFRTTDVTGSIELPAGVEMVMPGDNISVNVNLIAPIAMSDGLRFAIREGGRTVGAGVVAKVIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.1065989847716, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.5239179954442, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.5239179954442, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=30.6843267108168, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=31.0961968680089, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=31.3432835820896, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=31.3432835820896, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=31.3432835820896, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=31.3664596273292, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI54607086, Length=347, Percent_Identity=29.6829971181556, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=29.5880149812734, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=81.0126582278481, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=81.0126582278481, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=29.6875, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=30.9090909090909, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789108, Length=333, Percent_Identity=29.1291291291291, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=147, Percent_Identity=34.0136054421769, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=54.3859649122807, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=44.6753246753247, Blast_Score=337, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=31.0267857142857, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=31.0267857142857, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=29.3577981651376, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=433, Percent_Identity=29.3302540415704, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=28.9044289044289, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=27.6548672566372, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=29, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=164, Percent_Identity=36.5853658536585, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=299, Percent_Identity=28.7625418060201, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=27.8969957081545, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=64.572864321608, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=31.6964285714286, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=31.6964285714286, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.1666666666667, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=467, Percent_Identity=25.6959314775161, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=28.7769784172662, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=195, Percent_Identity=31.2820512820513, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=29.7071129707113, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.4885496183206, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.4885496183206, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=53.1088082901554, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=30.2013422818792, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=30.2013422818792, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=29.5302013422819, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=29.5302013422819, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=29.5302013422819, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=29.5302013422819, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=31.9526627218935, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.4754098360656, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=30.8605341246291, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=29.3906810035842, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=29.3906810035842, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42925; Mature: 42794

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERVKPHINVGTIGHVDHGKTTLTAAITTILTRKFGGEAKSYAQIDSAPEERARG
CCCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHCC
ITINTSHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFMNKADMVDDAELLELVEMEIRELLSKYDFPGDDTPIIIGSALKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHC
GDKGDIGEAAILKLAEVLDSYIPEPQRAIDGAFIMPVEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEEHHHHHCCCCCCEEEECHHCCCEEE
VGDEIEIVGLRPTIKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREEVERGQVLAKPGS
ECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCC
ILPHTKFSAEIYVLSKEEGGRHTPFFAGYRPQFYFRTTDVTGSIELPAGVEMVMPGDNIS
CCCCCCCCEEEEEEECCCCCCCCCEECCCCCEEEEEEECCCEEEECCCCEEEEECCCCEE
VNVNLIAPIAMSDGLRFAIREGGRTVGAGVVAKVIE
EEEEEEEEEEECCCCEEEEECCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKSKFERVKPHINVGTIGHVDHGKTTLTAAITTILTRKFGGEAKSYAQIDSAPEERARG
CCCHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHCC
ITINTSHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFMNKADMVDDAELLELVEMEIRELLSKYDFPGDDTPIIIGSALKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHC
GDKGDIGEAAILKLAEVLDSYIPEPQRAIDGAFIMPVEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEEHHHHHCCCCCCEEEECHHCCCEEE
VGDEIEIVGLRPTIKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREEVERGQVLAKPGS
ECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCC
ILPHTKFSAEIYVLSKEEGGRHTPFFAGYRPQFYFRTTDVTGSIELPAGVEMVMPGDNIS
CCCCCCCCEEEEEEECCCCCCCCCEECCCCCEEEEEEECCCEEEECCCCEEEEECCCCEE
VNVNLIAPIAMSDGLRFAIREGGRTVGAGVVAKVIE
EEEEEEEEEEECCCCEEEEECCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA