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The map label for this gene is dusB [H]

Identifier: 114331206

GI number: 114331206

Start: 1263057

End: 1264070

Strand: Direct

Name: dusB [H]

Synonym: Neut_1208

Alternate gene names: 114331206

Gene position: 1263057-1264070 (Clockwise)

Preceding gene: 114331205

Following gene: 114331207

Centisome position: 47.46

GC content: 48.72

Gene sequence:

>1014_bases
ATGCAAATTGGTTCCTATACGCTTAAAAATAAATTGATTGTTGCCCCCATGGCAGGAGTGACAGATCGACCTTTCCGACA
ATTGTGCAAGCGGATGGGCGCTGCGATGGCGGTGTCTGAAATGATTTCCAGTAACTCCCTGTTATATGGCTCTGAAAAAA
CCCTGCGCCGTGCTCGTCATGAAGGAGAGGTGAAACCAGTTTCGGTACAGATTGCAGGCGCTGATCCAGCCATGATGGCA
CAAGCTGCGCGCCACAACGCGGATCATGGTGCCGAAATCATTGATATCAACATGGGGTGTCCAGCCAAAAAAATATGCAA
TGTCATGGCAGGATCAGCTCTGCTGAAAGATGAAGATCTGGTCAGCAAAATTCTTGGGGCAGTCGTGCAGGCTGTGGATA
TTCCAGTCACCCTGAAAATCAGAACCGGCTGGGATACGCAGCATAAAAATGCAGTTGCAGTTGCCCGAATTGCTGAAAGT
GCAGGTATCCAAGCACTGGCTATACACGGGCGTACACGTGCTTGTGCCTATCGGGGGCAGGCTGAGTATGACACTATCGC
GACCGTCAAAGCAGCCATCAATATTCCTGTGATTGCCAATGGCGATATCAATACACCGGAGAAAGCTAGAGAAGTACTTG
AATACACGGGAGTGGATGCTGTCATGATAGGTCGTGCTGCCCAGGGTAGACCTTGGATTTTTCGGGAAATCAATCATTAT
CTGGCAACAGGCAATTTTTTACCTTTACCTGAAGTAGCTGAAATCCATCGTGTTTTGATTGACCATCTGCAAGAGCTTTA
TCAGTTCTACGGTGAATATTCAGGCGTGCGAATCGCCCGCAAACATATCTCCTGGTATACCCGTGGGTTGGCAGGTTCTG
CTGTTTTTCGCCATGCCATGAACCGGCTTGCATCGGTTGAGGAACAATTGGCAGCCACCCATCAGTTTTTCCATGAGCAG
GCTGATCGGAGCCAAACACTTACCTATATTGACGAAGAGATCCTTGAGGCATGA

Upstream 100 bases:

>100_bases
AATTTGGATCTGATTTTTCAATAATTTTCATTAACAGCCAAAACCAGCTAGCAGCTATGGAATTTTTCTTCTGACGTTTT
TAACCTGGTTTTACCTGTTT

Downstream 100 bases:

>100_bases
CTGTTATTAATGAAAATGAAATTGCACTCTGTGTTCGTAAAGCAGTAGAGGCTTACTTTCGTGATCTGGAGGGTGAAAAA
CCATGCCCTATTTATGAAAT

Product: putative TIM-barrel protein, nifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQIGSYTLKNKLIVAPMAGVTDRPFRQLCKRMGAAMAVSEMISSNSLLYGSEKTLRRARHEGEVKPVSVQIAGADPAMMA
QAARHNADHGAEIIDINMGCPAKKICNVMAGSALLKDEDLVSKILGAVVQAVDIPVTLKIRTGWDTQHKNAVAVARIAES
AGIQALAIHGRTRACAYRGQAEYDTIATVKAAINIPVIANGDINTPEKAREVLEYTGVDAVMIGRAAQGRPWIFREINHY
LATGNFLPLPEVAEIHRVLIDHLQELYQFYGEYSGVRIARKHISWYTRGLAGSAVFRHAMNRLASVEEQLAATHQFFHEQ
ADRSQTLTYIDEEILEA

Sequences:

>Translated_337_residues
MQIGSYTLKNKLIVAPMAGVTDRPFRQLCKRMGAAMAVSEMISSNSLLYGSEKTLRRARHEGEVKPVSVQIAGADPAMMA
QAARHNADHGAEIIDINMGCPAKKICNVMAGSALLKDEDLVSKILGAVVQAVDIPVTLKIRTGWDTQHKNAVAVARIAES
AGIQALAIHGRTRACAYRGQAEYDTIATVKAAINIPVIANGDINTPEKAREVLEYTGVDAVMIGRAAQGRPWIFREINHY
LATGNFLPLPEVAEIHRVLIDHLQELYQFYGEYSGVRIARKHISWYTRGLAGSAVFRHAMNRLASVEEQLAATHQFFHEQ
ADRSQTLTYIDEEILEA
>Mature_337_residues
MQIGSYTLKNKLIVAPMAGVTDRPFRQLCKRMGAAMAVSEMISSNSLLYGSEKTLRRARHEGEVKPVSVQIAGADPAMMA
QAARHNADHGAEIIDINMGCPAKKICNVMAGSALLKDEDLVSKILGAVVQAVDIPVTLKIRTGWDTQHKNAVAVARIAES
AGIQALAIHGRTRACAYRGQAEYDTIATVKAAINIPVIANGDINTPEKAREVLEYTGVDAVMIGRAAQGRPWIFREINHY
LATGNFLPLPEVAEIHRVLIDHLQELYQFYGEYSGVRIARKHISWYTRGLAGSAVFRHAMNRLASVEEQLAATHQFFHEQ
ADRSQTLTYIDEEILEA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=34.453781512605, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=34.453781512605, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=30.9917355371901, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=30.9734513274336, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=30.4, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=58.2554517133956, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1788462, Length=252, Percent_Identity=31.3492063492063, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI145693211, Length=241, Percent_Identity=28.2157676348548, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=247, Percent_Identity=32.3886639676113, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=217, Percent_Identity=30.8755760368664, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=264, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=253, Percent_Identity=29.2490118577075, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=258, Percent_Identity=31.7829457364341, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=30.0429184549356, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=27.5555555555556, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585320, Length=237, Percent_Identity=34.5991561181435, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24580595, Length=232, Percent_Identity=31.8965517241379, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920448, Length=232, Percent_Identity=31.8965517241379, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45549423, Length=283, Percent_Identity=29.3286219081272, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=242, Percent_Identity=28.099173553719, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37013; Mature: 37013

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGSYTLKNKLIVAPMAGVTDRPFRQLCKRMGAAMAVSEMISSNSLLYGSEKTLRRARH
CCCCCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHH
EGEVKPVSVQIAGADPAMMAQAARHNADHGAEIIDINMGCPAKKICNVMAGSALLKDEDL
CCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHCCHHHH
VSKILGAVVQAVDIPVTLKIRTGWDTQHKNAVAVARIAESAGIQALAIHGRTRACAYRGQ
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHCCCC
AEYDTIATVKAAINIPVIANGDINTPEKAREVLEYTGVDAVMIGRAAQGRPWIFREINHY
CCHHHHHHHHHHEECCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
LATGNFLPLPEVAEIHRVLIDHLQELYQFYGEYSGVRIARKHISWYTRGLAGSAVFRHAM
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
NRLASVEEQLAATHQFFHEQADRSQTLTYIDEEILEA
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGSYTLKNKLIVAPMAGVTDRPFRQLCKRMGAAMAVSEMISSNSLLYGSEKTLRRARH
CCCCCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHH
EGEVKPVSVQIAGADPAMMAQAARHNADHGAEIIDINMGCPAKKICNVMAGSALLKDEDL
CCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHCCHHHH
VSKILGAVVQAVDIPVTLKIRTGWDTQHKNAVAVARIAESAGIQALAIHGRTRACAYRGQ
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHCCCC
AEYDTIATVKAAINIPVIANGDINTPEKAREVLEYTGVDAVMIGRAAQGRPWIFREINHY
CCHHHHHHHHHHEECCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
LATGNFLPLPEVAEIHRVLIDHLQELYQFYGEYSGVRIARKHISWYTRGLAGSAVFRHAM
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
NRLASVEEQLAATHQFFHEQADRSQTLTYIDEEILEA
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]