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The map label for this gene is cysB [H]

Identifier: 114331184

GI number: 114331184

Start: 1239012

End: 1239941

Strand: Reverse

Name: cysB [H]

Synonym: Neut_1186

Alternate gene names: 114331184

Gene position: 1239941-1239012 (Counterclockwise)

Preceding gene: 114331192

Following gene: 114331183

Centisome position: 46.6

GC content: 45.05

Gene sequence:

>930_bases
ATGAAACTCCAGCAATTGAGATACCTTCGTGAAATTGCAAAACAAAAGCTGAATTTATCCAAGGCTGCGAATGCTCTGCA
TACTTCACAACCTGGGGTCAGTCGACAAATCCAGCTATTGGAACAAGAGTTGGGGGTCGAGATTTTTACACGCCACGGTA
AACGTCTTACCAGTATCACTCAGCCAGGGCTTGCGATCCTGGAAACTGCCCAACGAATGCTGCAGGACGCAGATAACCTG
AAAAAAATTGGAAATGAATTTAATGATAAGGAGAGCGGTACATTTGTTATTGCAACTACTCACACACAAGCACGGTATGT
GCTCCCTCCTGTAATCAAGCGCTTTATCGAACGCCACCCAAAAATCAGATTATCCTTGCGACAGGGAAGCCCTATAGAAA
TATCCACCTGGGTTTCATCTGGTGAAGCCGATTTGGCTATCGCAACCGAAGCCATCGAATTATTCGACGAACTCGTTATG
TTGCCTTGTTATGAATGGAATCGTTGTGTCGTCGTTCCGCCTCGGCATCCACTACTCCAACTTAAGGAGTTAACGCTTGA
ATCCATCGCTCAATACACAATCATTACCTACGATTTTGCTTTTACCGGTCGATCCAAAATCAATCAGGCATTTGAATCCA
GAGGATTAAAGCCCAACGTGGTACTGACAGCACTTGACGCGGATGTAATCAAAACCTATGTAGAAATCGGTTTGGGCGTA
GGCATTCTGGCAAAGATGGCCTTTGATCCGGCTCGCGATAAAAAGTTGCGGGCCATTGATGCCGCACATCTGTTTGAACC
CAGTACTACTCGCATCGGTATCTGTCGCAACAGCTACATCCGCCACTACATTTACGATTTTATTGAGATGTTTGCGACAC
ATCTTGATACAGAAACGATCAATAGGCTGCTCAGAAATCCTGAAAACTAA

Upstream 100 bases:

>100_bases
CATTTTTACATTGCACTTTAAACATATATGCAAGCATAACAATAATATTTATAATATAAAAGAATATTAAATTAATTATA
TATTATAGTTTGTTATATAA

Downstream 100 bases:

>100_bases
CTGCTATTGGTAGTAACAATTGGCATAGACAAAAAGGCTCCCACCCTGTCCGGATTGATGACTAGAACGCTTTCTGGTAG
ACTCCGCCCCTTAAAATTTC

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKLQQLRYLREIAKQKLNLSKAANALHTSQPGVSRQIQLLEQELGVEIFTRHGKRLTSITQPGLAILETAQRMLQDADNL
KKIGNEFNDKESGTFVIATTHTQARYVLPPVIKRFIERHPKIRLSLRQGSPIEISTWVSSGEADLAIATEAIELFDELVM
LPCYEWNRCVVVPPRHPLLQLKELTLESIAQYTIITYDFAFTGRSKINQAFESRGLKPNVVLTALDADVIKTYVEIGLGV
GILAKMAFDPARDKKLRAIDAAHLFEPSTTRIGICRNSYIRHYIYDFIEMFATHLDTETINRLLRNPEN

Sequences:

>Translated_309_residues
MKLQQLRYLREIAKQKLNLSKAANALHTSQPGVSRQIQLLEQELGVEIFTRHGKRLTSITQPGLAILETAQRMLQDADNL
KKIGNEFNDKESGTFVIATTHTQARYVLPPVIKRFIERHPKIRLSLRQGSPIEISTWVSSGEADLAIATEAIELFDELVM
LPCYEWNRCVVVPPRHPLLQLKELTLESIAQYTIITYDFAFTGRSKINQAFESRGLKPNVVLTALDADVIKTYVEIGLGV
GILAKMAFDPARDKKLRAIDAAHLFEPSTTRIGICRNSYIRHYIYDFIEMFATHLDTETINRLLRNPEN
>Mature_309_residues
MKLQQLRYLREIAKQKLNLSKAANALHTSQPGVSRQIQLLEQELGVEIFTRHGKRLTSITQPGLAILETAQRMLQDADNL
KKIGNEFNDKESGTFVIATTHTQARYVLPPVIKRFIERHPKIRLSLRQGSPIEISTWVSSGEADLAIATEAIELFDELVM
LPCYEWNRCVVVPPRHPLLQLKELTLESIAQYTIITYDFAFTGRSKINQAFESRGLKPNVVLTALDADVIKTYVEIGLGV
GILAKMAFDPARDKKLRAIDAAHLFEPSTTRIGICRNSYIRHYIYDFIEMFATHLDTETINRLLRNPEN

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=301, Percent_Identity=52.4916943521595, Blast_Score=345, Evalue=1e-96,
Organism=Escherichia coli, GI1788296, Length=302, Percent_Identity=47.0198675496689, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI157672245, Length=224, Percent_Identity=30.8035714285714, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788887, Length=253, Percent_Identity=30.0395256916996, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790399, Length=263, Percent_Identity=25.4752851711027, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=24.7933884297521, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789204, Length=235, Percent_Identity=26.8085106382979, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790208, Length=305, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=26.4044943820225, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=245, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=234, Percent_Identity=27.3504273504274, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=23.3576642335766, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35126; Mature: 35126

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYLREIAKQKLNLSKAANALHTSQPGVSRQIQLLEQELGVEIFTRHGKRLTSIT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCCHHHHHC
QPGLAILETAQRMLQDADNLKKIGNEFNDKESGTFVIATTHTQARYVLPPVIKRFIERHP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KIRLSLRQGSPIEISTWVSSGEADLAIATEAIELFDELVMLPCYEWNRCVVVPPRHPLLQ
CEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
LKELTLESIAQYTIITYDFAFTGRSKINQAFESRGLKPNVVLTALDADVIKTYVEIGLGV
HHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCH
GILAKMAFDPARDKKLRAIDAAHLFEPSTTRIGICRNSYIRHYIYDFIEMFATHLDTETI
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHH
NRLLRNPEN
HHHHCCCCC
>Mature Secondary Structure
MKLQQLRYLREIAKQKLNLSKAANALHTSQPGVSRQIQLLEQELGVEIFTRHGKRLTSIT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCCHHHHHC
QPGLAILETAQRMLQDADNLKKIGNEFNDKESGTFVIATTHTQARYVLPPVIKRFIERHP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KIRLSLRQGSPIEISTWVSSGEADLAIATEAIELFDELVMLPCYEWNRCVVVPPRHPLLQ
CEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
LKELTLESIAQYTIITYDFAFTGRSKINQAFESRGLKPNVVLTALDADVIKTYVEIGLGV
HHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCH
GILAKMAFDPARDKKLRAIDAAHLFEPSTTRIGICRNSYIRHYIYDFIEMFATHLDTETI
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHH
NRLLRNPEN
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]