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The map label for this gene is cysJ [H]

Identifier: 114331183

GI number: 114331183

Start: 1236632

End: 1238467

Strand: Reverse

Name: cysJ [H]

Synonym: Neut_1185

Alternate gene names: 114331183

Gene position: 1238467-1236632 (Counterclockwise)

Preceding gene: 114331184

Following gene: 114331182

Centisome position: 46.54

GC content: 48.8

Gene sequence:

>1836_bases
ATGACGGTATCACAATTCTCAGGAGCCGCTCTGACTGCAGAACAGTGGCGTCTTGTCGAAGCACTAAGCTCTTCTCTTAC
ACCTGAACATGCGTATTGGATCAGCGGTTACTTTGCTGGCATTGGGTCAGCCTTGTTACACAAGGGTACTACTGCGGCTC
AATCTCCAGATACACGCATTTCCACAGCAGTAGTACAAGCAGAACCAGTTGCAGCTCGTTCACTTACAATCCTTCATATC
AGTGAAACCGGTAACAGCACCGAACTGGCAATCCGTTTGGCAGCACTGGCTATTGAACAAGGACTCAACCCGACATTGGT
TGACATAGCTGATTACAAAGTTCGCAAACTCAAAGAAGAGCAGGATCTTCTGATCATCACCTCCACCCATGGAGAAGGAG
ACCCACCTCAGTCTGGAATGGAGTTTTTTGAATTAATCGAAAGCCGCAAGGCGCCAGCACTACCTGAACTACGTTACGCC
ATACTTGCGCTGGGCGACATGAGCTATGAACATTTTTGTGGCGCCGGCAAACGTCTTGATGAGCGTTTCGAGGAACTGGG
CGCAAAACGGCTGCAGCCACGCATTGACTGTGATGTGGATTATGAAGAACTGGCTGCTGCATGGAGTAGCAATATACTGG
CGCTACTCACCGCCGAGAAAAGAGCGGCGACATCATCCGCCGCATCACAACTAGAGACAACAGGAAGTACTCAGCAAGCT
GATAATTCAGCTTACGGTAAACGAAACCCCTTCCCTGCAACTGTCATTGACAACATTGTTCTCTCGGGTCGCGGCTCTAC
CCGGGAAACACACCACATCGAAATATCACTTGCTGATTCCGCTTTAACCTATGAGCCGGGCGATGCGTTGGGCATCGTGG
CGAACAACGATCCTGTGATGGTTGAGGCACTACTGACCACGCTCGACATGAATCCAGGCACATCGCTGACCGTAAAGGGT
CAGGTAACAACACTCTTTGAAGCGCTTAGCAAATTATTCGAAATTACCACAGCAACACTGCGTTTTCTTGATCACTGGGG
TGAATTGACTGGCACAACCGATATTTTTACTGCAATGGATAATGTCGAACGTGCGGCATTCCTTCATGAGCATCATATTA
TTGATATTATTCGCGCTTTTCCGCTCAAAGGTCTTGATCCACAGCGTTTTATCGACGGGTTGCGCCCTCTGCAACCGCGC
CTCTACTCTATTTCCTCCAGCCTGGCGATTTGCCCTGATGAGGCACATATCACTGTCGCACCAGTACGTTATAAGCTGCA
TGGAGAAATTCGTTCAGGTGTTGCATCCAGCCACCTTGCTGATCGTGCAAACGCTGATTCCATATTACCGGTCTACATTC
AGAGTAATCCATACTTTCGCCTGCCACCCAATGGCGAACCCATCATCATGATCGGCGCTGGTACCGGTGTGGCACCTTAC
CGTGCCTTTATGCAAGAACGCGAAGCAAATGGCGGTGGCCGTTCATGGTTATTTTTTGGAGAACGCAATTTCCGTGTGGA
TTTTCTTTATCAGACCGAGTGGCAGGACTGGTTAAAAAATAGCATTCTGACAAAAATGGATGTCGCTTTCTCACGAGATA
ACGCAGAGAAAACTTATGTTCAGCACCGTATTCATGAACATGCACACGATGTCTATGCTTGGTTGGAAGAAGGTGCACAT
ATTTATATATGTGGAGATGCGTTACGCCTGGCGCCTGATGTCCACAATACATTGGTCACCGTAATCGAAGAACAAGCAAG
CGTTCAGCGCGAAGAAGCAGAAGAGTATCTTCGCAACCTGCAACGCGATGGGCGCTATCAGCGCGACGTTTATTGA

Upstream 100 bases:

>100_bases
TTTTAATTGATTTGCCATATCTACAAAATTTCAACCACCAGCTGGATATCTCATTTCTACGTATTTCTACGTGTGAAAAA
CTTCCAAAATATAAAATAAA

Downstream 100 bases:

>100_bases
GAATAGAAATGGCAGACACACTCCCTCCCACTGATCGCAGCTGCGATATTTCCCAACCACTTGAGCGCCTAAGCCCGGAT
GAGGCACTGAAGGCTGGAAG

Product: sulfite reductase (NADPH) flavoprotein, alpha chain

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MTVSQFSGAALTAEQWRLVEALSSSLTPEHAYWISGYFAGIGSALLHKGTTAAQSPDTRISTAVVQAEPVAARSLTILHI
SETGNSTELAIRLAALAIEQGLNPTLVDIADYKVRKLKEEQDLLIITSTHGEGDPPQSGMEFFELIESRKAPALPELRYA
ILALGDMSYEHFCGAGKRLDERFEELGAKRLQPRIDCDVDYEELAAAWSSNILALLTAEKRAATSSAASQLETTGSTQQA
DNSAYGKRNPFPATVIDNIVLSGRGSTRETHHIEISLADSALTYEPGDALGIVANNDPVMVEALLTTLDMNPGTSLTVKG
QVTTLFEALSKLFEITTATLRFLDHWGELTGTTDIFTAMDNVERAAFLHEHHIIDIIRAFPLKGLDPQRFIDGLRPLQPR
LYSISSSLAICPDEAHITVAPVRYKLHGEIRSGVASSHLADRANADSILPVYIQSNPYFRLPPNGEPIIMIGAGTGVAPY
RAFMQEREANGGGRSWLFFGERNFRVDFLYQTEWQDWLKNSILTKMDVAFSRDNAEKTYVQHRIHEHAHDVYAWLEEGAH
IYICGDALRLAPDVHNTLVTVIEEQASVQREEAEEYLRNLQRDGRYQRDVY

Sequences:

>Translated_611_residues
MTVSQFSGAALTAEQWRLVEALSSSLTPEHAYWISGYFAGIGSALLHKGTTAAQSPDTRISTAVVQAEPVAARSLTILHI
SETGNSTELAIRLAALAIEQGLNPTLVDIADYKVRKLKEEQDLLIITSTHGEGDPPQSGMEFFELIESRKAPALPELRYA
ILALGDMSYEHFCGAGKRLDERFEELGAKRLQPRIDCDVDYEELAAAWSSNILALLTAEKRAATSSAASQLETTGSTQQA
DNSAYGKRNPFPATVIDNIVLSGRGSTRETHHIEISLADSALTYEPGDALGIVANNDPVMVEALLTTLDMNPGTSLTVKG
QVTTLFEALSKLFEITTATLRFLDHWGELTGTTDIFTAMDNVERAAFLHEHHIIDIIRAFPLKGLDPQRFIDGLRPLQPR
LYSISSSLAICPDEAHITVAPVRYKLHGEIRSGVASSHLADRANADSILPVYIQSNPYFRLPPNGEPIIMIGAGTGVAPY
RAFMQEREANGGGRSWLFFGERNFRVDFLYQTEWQDWLKNSILTKMDVAFSRDNAEKTYVQHRIHEHAHDVYAWLEEGAH
IYICGDALRLAPDVHNTLVTVIEEQASVQREEAEEYLRNLQRDGRYQRDVY
>Mature_610_residues
TVSQFSGAALTAEQWRLVEALSSSLTPEHAYWISGYFAGIGSALLHKGTTAAQSPDTRISTAVVQAEPVAARSLTILHIS
ETGNSTELAIRLAALAIEQGLNPTLVDIADYKVRKLKEEQDLLIITSTHGEGDPPQSGMEFFELIESRKAPALPELRYAI
LALGDMSYEHFCGAGKRLDERFEELGAKRLQPRIDCDVDYEELAAAWSSNILALLTAEKRAATSSAASQLETTGSTQQAD
NSAYGKRNPFPATVIDNIVLSGRGSTRETHHIEISLADSALTYEPGDALGIVANNDPVMVEALLTTLDMNPGTSLTVKGQ
VTTLFEALSKLFEITTATLRFLDHWGELTGTTDIFTAMDNVERAAFLHEHHIIDIIRAFPLKGLDPQRFIDGLRPLQPRL
YSISSSLAICPDEAHITVAPVRYKLHGEIRSGVASSHLADRANADSILPVYIQSNPYFRLPPNGEPIIMIGAGTGVAPYR
AFMQEREANGGGRSWLFFGERNFRVDFLYQTEWQDWLKNSILTKMDVAFSRDNAEKTYVQHRIHEHAHDVYAWLEEGAHI
YICGDALRLAPDVHNTLVTVIEEQASVQREEAEEYLRNLQRDGRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=623, Percent_Identity=29.2134831460674, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=28.5953177257525, Blast_Score=204, Evalue=3e-52,
Organism=Homo sapiens, GI10835173, Length=717, Percent_Identity=26.2203626220363, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI7657393, Length=605, Percent_Identity=26.9421487603306, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI40254422, Length=716, Percent_Identity=26.2569832402235, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI221316705, Length=614, Percent_Identity=26.5472312703583, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI221316709, Length=598, Percent_Identity=27.0903010033445, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI221316707, Length=526, Percent_Identity=28.1368821292776, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI169790956, Length=257, Percent_Identity=34.2412451361868, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI169790958, Length=257, Percent_Identity=34.2412451361868, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI1789123, Length=604, Percent_Identity=40.2317880794702, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1789154, Length=108, Percent_Identity=34.2592592592593, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554134, Length=599, Percent_Identity=28.5475792988314, Blast_Score=212, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17566446, Length=608, Percent_Identity=25.3289473684211, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531441, Length=397, Percent_Identity=24.9370277078086, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321832, Length=663, Percent_Identity=28.3559577677225, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321143, Length=371, Percent_Identity=27.2237196765499, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325305, Length=634, Percent_Identity=22.397476340694, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322597, Length=150, Percent_Identity=32.6666666666667, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17137192, Length=612, Percent_Identity=30.2287581699346, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24582192, Length=521, Percent_Identity=29.9424184261036, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI78706872, Length=658, Percent_Identity=27.0516717325228, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24583543, Length=658, Percent_Identity=27.0516717325228, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI78706876, Length=658, Percent_Identity=27.0516717325228, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24660907, Length=599, Percent_Identity=22.0367278797997, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24660903, Length=599, Percent_Identity=22.0367278797997, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647438, Length=433, Percent_Identity=24.9422632794457, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 67666; Mature: 67535

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSQFSGAALTAEQWRLVEALSSSLTPEHAYWISGYFAGIGSALLHKGTTAAQSPDTRI
CCCCCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
STAVVQAEPVAARSLTILHISETGNSTELAIRLAALAIEQGLNPTLVDIADYKVRKLKEE
EEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCC
QDLLIITSTHGEGDPPQSGMEFFELIESRKAPALPELRYAILALGDMSYEHFCGAGKRLD
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHH
ERFEELGAKRLQPRIDCDVDYEELAAAWSSNILALLTAEKRAATSSAASQLETTGSTQQA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCC
DNSAYGKRNPFPATVIDNIVLSGRGSTRETHHIEISLADSALTYEPGDALGIVANNDPVM
CCCCCCCCCCCCHHHHHHEEEECCCCCCCEEEEEEEEECCEEEECCCCEEEEEECCCCHH
VEALLTTLDMNPGTSLTVKGQVTTLFEALSKLFEITTATLRFLDHWGELTGTTDIFTAMD
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NVERAAFLHEHHIIDIIRAFPLKGLDPQRFIDGLRPLQPRLYSISSSLAICPDEAHITVA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHCCCEEECCCCCEEEEE
PVRYKLHGEIRSGVASSHLADRANADSILPVYIQSNPYFRLPPNGEPIIMIGAGTGVAPY
EEEEEEECHHHCCHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCEEEEEECCCCCHHH
RAFMQEREANGGGRSWLFFGERNFRVDFLYQTEWQDWLKNSILTKMDVAFSRDNAEKTYV
HHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHEECCCCHHHHHH
QHRIHEHAHDVYAWLEEGAHIYICGDALRLAPDVHNTLVTVIEEQASVQREEAEEYLRNL
HHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRDGRYQRDVY
HHCCCCCCCCC
>Mature Secondary Structure 
TVSQFSGAALTAEQWRLVEALSSSLTPEHAYWISGYFAGIGSALLHKGTTAAQSPDTRI
CCCCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
STAVVQAEPVAARSLTILHISETGNSTELAIRLAALAIEQGLNPTLVDIADYKVRKLKEE
EEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCC
QDLLIITSTHGEGDPPQSGMEFFELIESRKAPALPELRYAILALGDMSYEHFCGAGKRLD
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHH
ERFEELGAKRLQPRIDCDVDYEELAAAWSSNILALLTAEKRAATSSAASQLETTGSTQQA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCC
DNSAYGKRNPFPATVIDNIVLSGRGSTRETHHIEISLADSALTYEPGDALGIVANNDPVM
CCCCCCCCCCCCHHHHHHEEEECCCCCCCEEEEEEEEECCEEEECCCCEEEEEECCCCHH
VEALLTTLDMNPGTSLTVKGQVTTLFEALSKLFEITTATLRFLDHWGELTGTTDIFTAMD
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NVERAAFLHEHHIIDIIRAFPLKGLDPQRFIDGLRPLQPRLYSISSSLAICPDEAHITVA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHCCCEEECCCCCEEEEE
PVRYKLHGEIRSGVASSHLADRANADSILPVYIQSNPYFRLPPNGEPIIMIGAGTGVAPY
EEEEEEECHHHCCHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCEEEEEECCCCCHHH
RAFMQEREANGGGRSWLFFGERNFRVDFLYQTEWQDWLKNSILTKMDVAFSRDNAEKTYV
HHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHEECCCCHHHHHH
QHRIHEHAHDVYAWLEEGAHIYICGDALRLAPDVHNTLVTVIEEQASVQREEAEEYLRNL
HHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRDGRYQRDVY
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]