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The map label for this gene is ipk

Identifier: 114331137

GI number: 114331137

Start: 1178299

End: 1179135

Strand: Direct

Name: ipk

Synonym: Neut_1139

Alternate gene names: 114331137

Gene position: 1178299-1179135 (Clockwise)

Preceding gene: 114331136

Following gene: 114331138

Centisome position: 44.28

GC content: 48.98

Gene sequence:

>837_bases
ATGAACACGTTTCCAGCACCGGCTAAACTTAACCTGTTTTTGCATGTAATCGGACGGCGAGATGACGGTTACCATTTGCT
GCAGACAGTTTTCCGTTTTATCGGCTATTCAGATCAACTGGGCTTTGATGTTACCAATGATGGCGTTATCAGACACCTGA
ATCTGGTTCCCGGCCTGACAGATACGGATGATCTATGTGTTCGGGCGGCAAAACTACTGCAGAAGAGATCCGGTAAAGAA
ATGCTCGGAGTCGGAATTCATCTGAGCAAGAATATTCCACTTGGAGGAGGATTGGGGGGGGGAAGTTCTGACGCTGCTAC
AACATTAATCGTTCTGAACAGATTGTGGGGAATTAACTGGGGGCGAGAACGTTTGATGGCATTGGGGCTGGAACTGGGCG
CTGATGTTCCGATATTTATCTATGGCAGGAACGCATTTGCTGAAGGAGTTGGTGAAAAACTGGAGGTGATTAACTTGCCA
CCCGCATGGTATGTCGTTCTGACGCCACCTGCTCCGATTTCCACAGCGGCAGTTTTTGCCAGCAGGGAGTTGACACGAAA
CACGATTCCCATCAAAATGGCGGCCTTTTCCATGGCGCAGGGACATAATGATCTTGAGCTTGTCGCGATGCGTTTGCAGC
CTGTTATCGCCGAATGGCTGGATTGGTTAAAAGGGCGGCATGGATCAACAAAAGTGGCAATGAGCGGTTCCGGATCATGT
GTGTTTGCAGAGTTTCCTTCCGAATCTGCTGCGCGAGAGGTACTCAGACAATTGCCTGATAGTATGAGTGGTTTTATCGC
ACCAGGGCTTGCAAGACATCCTTTGTCGGATTTTTAG

Upstream 100 bases:

>100_bases
AAAGATGAAGCTGATTGTTGATCAATGGGAAAAAGAGGCAAAGGGCGTTACTGGAGTGGACAGGAAGCTGCCGTAACAGT
TTTTAATTCTCATATAGGGT

Downstream 100 bases:

>100_bases
AATATTTTTTGGGGAGTCGCCAAGTGGTAAGGCACCGGATTTTGATTCCGGCATTCGTAGGTTCGATCCCTACCTCCCCA
GCCAAATCTCAGTGAATCTG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNTFPAPAKLNLFLHVIGRRDDGYHLLQTVFRFIGYSDQLGFDVTNDGVIRHLNLVPGLTDTDDLCVRAAKLLQKRSGKE
MLGVGIHLSKNIPLGGGLGGGSSDAATTLIVLNRLWGINWGRERLMALGLELGADVPIFIYGRNAFAEGVGEKLEVINLP
PAWYVVLTPPAPISTAAVFASRELTRNTIPIKMAAFSMAQGHNDLELVAMRLQPVIAEWLDWLKGRHGSTKVAMSGSGSC
VFAEFPSESAAREVLRQLPDSMSGFIAPGLARHPLSDF

Sequences:

>Translated_278_residues
MNTFPAPAKLNLFLHVIGRRDDGYHLLQTVFRFIGYSDQLGFDVTNDGVIRHLNLVPGLTDTDDLCVRAAKLLQKRSGKE
MLGVGIHLSKNIPLGGGLGGGSSDAATTLIVLNRLWGINWGRERLMALGLELGADVPIFIYGRNAFAEGVGEKLEVINLP
PAWYVVLTPPAPISTAAVFASRELTRNTIPIKMAAFSMAQGHNDLELVAMRLQPVIAEWLDWLKGRHGSTKVAMSGSGSC
VFAEFPSESAAREVLRQLPDSMSGFIAPGLARHPLSDF
>Mature_278_residues
MNTFPAPAKLNLFLHVIGRRDDGYHLLQTVFRFIGYSDQLGFDVTNDGVIRHLNLVPGLTDTDDLCVRAAKLLQKRSGKE
MLGVGIHLSKNIPLGGGLGGGSSDAATTLIVLNRLWGINWGRERLMALGLELGADVPIFIYGRNAFAEGVGEKLEVINLP
PAWYVVLTPPAPISTAAVFASRELTRNTIPIKMAAFSMAQGHNDLELVAMRLQPVIAEWLDWLKGRHGSTKVAMSGSGSC
VFAEFPSESAAREVLRQLPDSMSGFIAPGLARHPLSDF

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=277, Percent_Identity=45.1263537906137, Blast_Score=210, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_NITEC (Q0AGY8)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747359.1
- ProteinModelPortal:   Q0AGY8
- SMR:   Q0AGY8
- STRING:   Q0AGY8
- GeneID:   4274104
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1139
- NMPDR:   fig|335283.3.peg.978
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   Q0AGY8
- ProtClustDB:   PRK00343
- BioCyc:   NEUT335283:NEUT_1139-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30129; Mature: 30129

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFPAPAKLNLFLHVIGRRDDGYHLLQTVFRFIGYSDQLGFDVTNDGVIRHLNLVPGLT
CCCCCCCHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCEEEEECCCCCC
DTDDLCVRAAKLLQKRSGKEMLGVGIHLSKNIPLGGGLGGGSSDAATTLIVLNRLWGINW
CHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
GRERLMALGLELGADVPIFIYGRNAFAEGVGEKLEVINLPPAWYVVLTPPAPISTAAVFA
CHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHHHH
SRELTRNTIPIKMAAFSMAQGHNDLELVAMRLQPVIAEWLDWLKGRHGSTKVAMSGSGSC
HHHHHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
VFAEFPSESAAREVLRQLPDSMSGFIAPGLARHPLSDF
EEEECCCHHHHHHHHHHCCHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure
MNTFPAPAKLNLFLHVIGRRDDGYHLLQTVFRFIGYSDQLGFDVTNDGVIRHLNLVPGLT
CCCCCCCHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCEEEEECCCCCC
DTDDLCVRAAKLLQKRSGKEMLGVGIHLSKNIPLGGGLGGGSSDAATTLIVLNRLWGINW
CHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
GRERLMALGLELGADVPIFIYGRNAFAEGVGEKLEVINLPPAWYVVLTPPAPISTAAVFA
CHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHHHH
SRELTRNTIPIKMAAFSMAQGHNDLELVAMRLQPVIAEWLDWLKGRHGSTKVAMSGSGSC
HHHHHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
VFAEFPSESAAREVLRQLPDSMSGFIAPGLARHPLSDF
EEEECCCHHHHHHHHHHCCHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA