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The map label for this gene is pilD [H]

Identifier: 114331043

GI number: 114331043

Start: 1082363

End: 1083223

Strand: Direct

Name: pilD [H]

Synonym: Neut_1044

Alternate gene names: 114331043

Gene position: 1082363-1083223 (Clockwise)

Preceding gene: 114331042

Following gene: 114331044

Centisome position: 40.67

GC content: 44.95

Gene sequence:

>861_bases
ATGAATATTTTTAATACCCTGCAAGCTTCACCAGCTTTTTTTATTTCATTTATCTCGCTTATTGGCCTGATTCTGGGAAG
TTTCCTGAATGTAGTGATTTACAGGTTGCCCGTGATGTTAAAACGTGAATGGATGCAACAATGTGCTGAGTTGCGTGGGG
AGCAGGTAAAAGTACTGCCGGCGTATAACCTGGCTATACCAGGCTCCGGTTGTTCACAGTGTGGTCATAAAATTTCCGTC
CTCGAAAATATTCCGATTATCAGCTACCTTCTTTTGCGCGGACACTGTTCAAGTTGCCAGGCTCGTATTCCATTTGATTA
TCCACTGGTTGAGGCTCTGACAGCTATTCTAAGCGGTTTTGCTGCGTGGCATTTTGGTTTTAGTGGGATTTTACTTGCTG
CGCTGATTTTTATTTGGGCAATGATTATTTTGACATTTATTGATCTGAATACCCAGCTTTTACCAGATAGTATTACACAG
CCGCTGTTATGGGCAGGCCTGATCGTGAATCTGCATAATGGTTTTGTAGATATCCATTCAGCTGTTATCGGTGCGGTTGC
AGGTTACCTGACACTCTGGAGTGTGTACTGGCTGTTCAAACTGATTACCGGTAAGGAAGGCATGGGGTATGGTGATTTCA
AGTTGCTGGCTGCAGTCGGTGCCTGGTTGGGTTGGCAATTACTGCCACTGGTAATCTTGCTTTCTTCTGTGATGGGGGCT
GCTATTGGTACCATCCTGATCCTGGCCGCTAATCGTTCAAAAGGTACAACACTTCCCTTTGGTCCTTATCTGGCAGGAGG
AGGATTAATTGCTTTGTTCTGGGGCAGTCAGATCAATCAGGCATACCTGGCCATGTTTTGA

Upstream 100 bases:

>100_bases
GGAATGGTGGTCGCCATGTATCTGCCTATTTTTAAAATGGGGCAGGTGGTCGGATAACCAGATAGTGCCATTTTCACGAC
TGAATCAGGACAGGCAGTGC

Downstream 100 bases:

>100_bases
CGTATGGGATTCATTATCGGGCTAACGGGTGGAATCGGCAGTGGCAAGACCAGTGTGGCTGATCTGTTCCAGGAGCTGGG
TATTGAAATTATTGATACTG

Product: prepilin peptidase

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNIFNTLQASPAFFISFISLIGLILGSFLNVVIYRLPVMLKREWMQQCAELRGEQVKVLPAYNLAIPGSGCSQCGHKISV
LENIPIISYLLLRGHCSSCQARIPFDYPLVEALTAILSGFAAWHFGFSGILLAALIFIWAMIILTFIDLNTQLLPDSITQ
PLLWAGLIVNLHNGFVDIHSAVIGAVAGYLTLWSVYWLFKLITGKEGMGYGDFKLLAAVGAWLGWQLLPLVILLSSVMGA
AIGTILILAANRSKGTTLPFGPYLAGGGLIALFWGSQINQAYLAMF

Sequences:

>Translated_286_residues
MNIFNTLQASPAFFISFISLIGLILGSFLNVVIYRLPVMLKREWMQQCAELRGEQVKVLPAYNLAIPGSGCSQCGHKISV
LENIPIISYLLLRGHCSSCQARIPFDYPLVEALTAILSGFAAWHFGFSGILLAALIFIWAMIILTFIDLNTQLLPDSITQ
PLLWAGLIVNLHNGFVDIHSAVIGAVAGYLTLWSVYWLFKLITGKEGMGYGDFKLLAAVGAWLGWQLLPLVILLSSVMGA
AIGTILILAANRSKGTTLPFGPYLAGGGLIALFWGSQINQAYLAMF
>Mature_286_residues
MNIFNTLQASPAFFISFISLIGLILGSFLNVVIYRLPVMLKREWMQQCAELRGEQVKVLPAYNLAIPGSGCSQCGHKISV
LENIPIISYLLLRGHCSSCQARIPFDYPLVEALTAILSGFAAWHFGFSGILLAALIFIWAMIILTFIDLNTQLLPDSITQ
PLLWAGLIVNLHNGFVDIHSAVIGAVAGYLTLWSVYWLFKLITGKEGMGYGDFKLLAAVGAWLGWQLLPLVILLSSVMGA
AIGTILILAANRSKGTTLPFGPYLAGGGLIALFWGSQINQAYLAMF

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=273, Percent_Identity=40.6593406593407, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789732, Length=159, Percent_Identity=49.0566037735849, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31139; Mature: 31139

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFNTLQASPAFFISFISLIGLILGSFLNVVIYRLPVMLKREWMQQCAELRGEQVKVLP
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AYNLAIPGSGCSQCGHKISVLENIPIISYLLLRGHCSSCQARIPFDYPLVEALTAILSGF
EECEECCCCCHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
AAWHFGFSGILLAALIFIWAMIILTFIDLNTQLLPDSITQPLLWAGLIVNLHNGFVDIHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
AVIGAVAGYLTLWSVYWLFKLITGKEGMGYGDFKLLAAVGAWLGWQLLPLVILLSSVMGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGTILILAANRSKGTTLPFGPYLAGGGLIALFWGSQINQAYLAMF
HHHHHHHEEECCCCCCCCCCCCHHHCCCEEEEEECCCCCHHHHHCC
>Mature Secondary Structure
MNIFNTLQASPAFFISFISLIGLILGSFLNVVIYRLPVMLKREWMQQCAELRGEQVKVLP
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AYNLAIPGSGCSQCGHKISVLENIPIISYLLLRGHCSSCQARIPFDYPLVEALTAILSGF
EECEECCCCCHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
AAWHFGFSGILLAALIFIWAMIILTFIDLNTQLLPDSITQPLLWAGLIVNLHNGFVDIHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
AVIGAVAGYLTLWSVYWLFKLITGKEGMGYGDFKLLAAVGAWLGWQLLPLVILLSSVMGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGTILILAANRSKGTTLPFGPYLAGGGLIALFWGSQINQAYLAMF
HHHHHHHEEECCCCCCCCCCCCHHHCCCEEEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]