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The map label for this gene is cysG

Identifier: 114331004

GI number: 114331004

Start: 1032865

End: 1034295

Strand: Direct

Name: cysG

Synonym: Neut_1002

Alternate gene names: 114331004

Gene position: 1032865-1034295 (Clockwise)

Preceding gene: 114331003

Following gene: 114331019

Centisome position: 38.81

GC content: 50.24

Gene sequence:

>1431_bases
ATGGATTACTTTCCAGTTTTTCTCAACATTAAACAGCGAAATTGTCTGATTGTTGGCGGAGGAAGTGTTGCAGCCCGCAA
GGCGAAGTTGCTACTGCGAGCAGGGGCGCATATCCATGTCGTTTCCCCCACAATTAATGCGGAATTTACTGGTTTGTTGC
AGCAATTCTCTTCAATCTCGCATAACGCTGAAACCTTCCGCCCGGATCACTTGCAGGGTAGGGTGCTGGTTATTGCCGCT
ACGCATGATCGAGCAACCAACCAGGCTGTCTCGGTTGCAGCCCGGAAAGCAGGTATTCCAGTAAACGTTGTCGATAATCC
GGATTTATGCACCTTTATCATGCCTTCCATTTTGGATCGCTCTCCGATTATCGTTGCGGTATCAAGCGGTGGCGGTTCTC
CAGTACTGGCAAGGCTGTTGCGTGCACGACTGGAAGCATTAATCCCCGCTGCTTATGGTCGACTGGCGACTTATGCTGCG
CAGTTTCGCGGGCAAGTCAGGCAGCATTTTTCTCGTCAGGAAAATCGTCGCTTCTTTTGGGAAAAAATGCTTCAAGGGCC
GTTTGCAGAAATGGTATTTGCCGGCAAGGATCAGGCTGCCCAGGATTATTTATTAGAAACCTTGAATAATAGTGTGGATC
AACCTCCGATCGGGGAAGTTTATCTGGTGGGAGCAGGCCCGGGAGACCCGGATCTGTTGACTTTCCGCGCTATGCGCCTG
ATGCAGCAGGCAGACGTGGTGATTTATGATCGGCTGGTTGCTCCGGCCATTTTGGATATGGTCAGACAGGATGCTGACCG
TATCTATGCCGGTAAGGAGCGTAATCGACATACTTTGCCGCAGACATCAATCAATAACCTGTTGATCAAGCTGGCGCAAG
AAGGTAAACGTGTGCTGCGGTTGAAAGGTGGCGATCCTTTTATTTTTGGACGAGGGGGCGAGGAAATCGAAACTTTGTCA
CAACATCAGATCCCATTCCAGGTTGTTCCCGGAATTACAGCCGCATCCGGTGTAGCTAGTTATGCAGGTATCCCTTTGAC
CCACCGGGATTACGCGCATTCCTGCGTTTTTGTAACCGGACATCTGAAAGACAACACTGTACAGCTTGACTGGTCAGCGC
TGGCGCGTCCTAACCAGACGATTGTCGTCTACATGGGATTGCTGGGCGTCTCCGAGTTATGCCGACAGTTGATTGCTCAT
GGTCTGCCAAAAACGACACCAGCTGCTATTATTCAGCAGGGTACAACCCCCAATCAGTGTGTTCTCACCGGCACACTTGC
AACGTTGCCGACAATCATTCAGGAAAATCCATTGAAGCCGCCAACACTCATTATTGTGGGAGAAGTGGTCAAGCTTCGTC
AGAGACTTGCCTGGTTCAATTCGACTTCAGAATCTTTGGGAAACACACCTGGCTACTCGAAACACCCTTAG

Upstream 100 bases:

>100_bases
AACCGGATATGTAATTCCGGAATAAGAGGTTATTACATCGTTCTGATTAACCTTTAACAGGCCAGACGGCTCCTTGTTTA
TTAAGCCCTATTTTGTCTGT

Downstream 100 bases:

>100_bases
CCTCAAACCTCAGCTGATTTGGTGACGGGCGTTATCTTAACGGCGGATCAAATAACGAATAGTGGGACCATCCTGCTGAA
TCTCCAGAACCTCGTAGCCA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDYFPVFLNIKQRNCLIVGGGSVAARKAKLLLRAGAHIHVVSPTINAEFTGLLQQFSSISHNAETFRPDHLQGRVLVIAA
THDRATNQAVSVAARKAGIPVNVVDNPDLCTFIMPSILDRSPIIVAVSSGGGSPVLARLLRARLEALIPAAYGRLATYAA
QFRGQVRQHFSRQENRRFFWEKMLQGPFAEMVFAGKDQAAQDYLLETLNNSVDQPPIGEVYLVGAGPGDPDLLTFRAMRL
MQQADVVIYDRLVAPAILDMVRQDADRIYAGKERNRHTLPQTSINNLLIKLAQEGKRVLRLKGGDPFIFGRGGEEIETLS
QHQIPFQVVPGITAASGVASYAGIPLTHRDYAHSCVFVTGHLKDNTVQLDWSALARPNQTIVVYMGLLGVSELCRQLIAH
GLPKTTPAAIIQQGTTPNQCVLTGTLATLPTIIQENPLKPPTLIIVGEVVKLRQRLAWFNSTSESLGNTPGYSKHP

Sequences:

>Translated_476_residues
MDYFPVFLNIKQRNCLIVGGGSVAARKAKLLLRAGAHIHVVSPTINAEFTGLLQQFSSISHNAETFRPDHLQGRVLVIAA
THDRATNQAVSVAARKAGIPVNVVDNPDLCTFIMPSILDRSPIIVAVSSGGGSPVLARLLRARLEALIPAAYGRLATYAA
QFRGQVRQHFSRQENRRFFWEKMLQGPFAEMVFAGKDQAAQDYLLETLNNSVDQPPIGEVYLVGAGPGDPDLLTFRAMRL
MQQADVVIYDRLVAPAILDMVRQDADRIYAGKERNRHTLPQTSINNLLIKLAQEGKRVLRLKGGDPFIFGRGGEEIETLS
QHQIPFQVVPGITAASGVASYAGIPLTHRDYAHSCVFVTGHLKDNTVQLDWSALARPNQTIVVYMGLLGVSELCRQLIAH
GLPKTTPAAIIQQGTTPNQCVLTGTLATLPTIIQENPLKPPTLIIVGEVVKLRQRLAWFNSTSESLGNTPGYSKHP
>Mature_476_residues
MDYFPVFLNIKQRNCLIVGGGSVAARKAKLLLRAGAHIHVVSPTINAEFTGLLQQFSSISHNAETFRPDHLQGRVLVIAA
THDRATNQAVSVAARKAGIPVNVVDNPDLCTFIMPSILDRSPIIVAVSSGGGSPVLARLLRARLEALIPAAYGRLATYAA
QFRGQVRQHFSRQENRRFFWEKMLQGPFAEMVFAGKDQAAQDYLLETLNNSVDQPPIGEVYLVGAGPGDPDLLTFRAMRL
MQQADVVIYDRLVAPAILDMVRQDADRIYAGKERNRHTLPQTSINNLLIKLAQEGKRVLRLKGGDPFIFGRGGEEIETLS
QHQIPFQVVPGITAASGVASYAGIPLTHRDYAHSCVFVTGHLKDNTVQLDWSALARPNQTIVVYMGLLGVSELCRQLIAH
GLPKTTPAAIIQQGTTPNQCVLTGTLATLPTIIQENPLKPPTLIIVGEVVKLRQRLAWFNSTSESLGNTPGYSKHP

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=463, Percent_Identity=50.9719222462203, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=33.0543933054393, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG_NITEC (Q0AHC1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747226.1
- ProteinModelPortal:   Q0AHC1
- SMR:   Q0AHC1
- STRING:   Q0AHC1
- GeneID:   4274154
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1002
- NMPDR:   fig|335283.3.peg.2152
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- OMA:   MCRRDAE
- PhylomeDB:   Q0AHC1
- ProtClustDB:   CLSK585370
- BioCyc:   NEUT335283:NEUT_1002-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 52003; Mature: 52003

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFPVFLNIKQRNCLIVGGGSVAARKAKLLLRAGAHIHVVSPTINAEFTGLLQQFSSIS
CCCEEEEEEEECCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHC
HNAETFRPDHLQGRVLVIAATHDRATNQAVSVAARKAGIPVNVVDNPDLCTFIMPSILDR
CCCHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCC
SPIIVAVSSGGGSPVLARLLRARLEALIPAAYGRLATYAAQFRGQVRQHFSRQENRRFFW
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKMLQGPFAEMVFAGKDQAAQDYLLETLNNSVDQPPIGEVYLVGAGPGDPDLLTFRAMRL
HHHHCCCHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
MQQADVVIYDRLVAPAILDMVRQDADRIYAGKERNRHTLPQTSINNLLIKLAQEGKRVLR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
LKGGDPFIFGRGGEEIETLSQHQIPFQVVPGITAASGVASYAGIPLTHRDYAHSCVFVTG
EECCCEEEECCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEE
HLKDNTVQLDWSALARPNQTIVVYMGLLGVSELCRQLIAHGLPKTTPAAIIQQGTTPNQC
ECCCCEEEEEHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCE
VLTGTLATLPTIIQENPLKPPTLIIVGEVVKLRQRLAWFNSTSESLGNTPGYSKHP
EEEEHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MDYFPVFLNIKQRNCLIVGGGSVAARKAKLLLRAGAHIHVVSPTINAEFTGLLQQFSSIS
CCCEEEEEEEECCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHC
HNAETFRPDHLQGRVLVIAATHDRATNQAVSVAARKAGIPVNVVDNPDLCTFIMPSILDR
CCCHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCC
SPIIVAVSSGGGSPVLARLLRARLEALIPAAYGRLATYAAQFRGQVRQHFSRQENRRFFW
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKMLQGPFAEMVFAGKDQAAQDYLLETLNNSVDQPPIGEVYLVGAGPGDPDLLTFRAMRL
HHHHCCCHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
MQQADVVIYDRLVAPAILDMVRQDADRIYAGKERNRHTLPQTSINNLLIKLAQEGKRVLR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
LKGGDPFIFGRGGEEIETLSQHQIPFQVVPGITAASGVASYAGIPLTHRDYAHSCVFVTG
EECCCEEEECCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEE
HLKDNTVQLDWSALARPNQTIVVYMGLLGVSELCRQLIAHGLPKTTPAAIIQQGTTPNQC
ECCCCEEEEEHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCE
VLTGTLATLPTIIQENPLKPPTLIIVGEVVKLRQRLAWFNSTSESLGNTPGYSKHP
EEEEHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA