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The map label for this gene is 114330957

Identifier: 114330957

GI number: 114330957

Start: 984940

End: 986250

Strand: Reverse

Name: 114330957

Synonym: Neut_0954

Alternate gene names: NA

Gene position: 986250-984940 (Counterclockwise)

Preceding gene: 114330971

Following gene: 114330956

Centisome position: 37.06

GC content: 47.29

Gene sequence:

>1311_bases
ATGCCGGTACCCCATCTCACCAATACCCTGAATGCACCCACCTTTGATCTGGAAAAACGTATTCTGACAGCCAAGCCAAC
TATCGAACACTGGTTTCGCAAACAATGGCAACTCCATACCGTGCCTTTTTATTGTTCGGTAGATCTGCGCAACAGCGGTT
TCAAACTGGCACCGGTTGATACCAACTTGTTTCCTGGTGGATTCAACAACCTTAATCCGACTTTCATCCCGCTATGCATT
CAGGCGATGATGAATGCCGTCGAAAAAATCTGCCCGGACGCACATAATGTTTTGCTGATTCCAGAAAATCACACCCGCAA
TATTTTCTATCTGCAAAATATTGCCATGTTGCAGTCCATCATGCAGCAGGCTGGTATGAATATCCGCATCGGCTCACTAT
TGCCAGGAATTGATCATCCTGTATCCGTACCACTACCTGGCGGTGAGCCTTTGTTACTGGAGCCAGTACAGCGCAAGGGG
AACAAACTGTTTGTTCAGGATTTTGACCCTTGTGCCATACTTCTGAATAACGATCTATCCAGCGGAGTACCTGAAATCCT
GCAAAATCTGGATCAGACAATTATCCCACCTCTCCACGCAGGCTGGGCCACTCGCCGTAAATCCTGGCATTTCAGCGCCT
ACGACAACGTAGCACAGGATTTCGCAGAGCTGATTGATATTGATCCCTGGCTTATTAACCCGTATTTTACCGCTTGCGGA
AAAGTTGATTTTCAAGAAAAAGAGGGACTGGATTGTGTCGCTGCAGCCGTAGATGAAATTCTGAACCAGATCAGGGAAAA
ATACACACAATATGGAATCAGTGAAGAGCCGTTTGTTATCGTCAAGGCTGATTCAGGCACTTACGGCATGGGAATCATGA
GTATCAAAGATGGTACTGAAGTCTACAAACTTAACCGCAAACAGCGCAACAAAATGACGACAATCAAGGAGGGGCTGGTT
GTGAAAAACGTACTGGTGCAAGAAGGAGTGCATACATTTGAGAACATTCACCAGGCCGTAGCGGAGCCCGTTATCTACAT
GATTGATCATCACGTAGTGGGAGGATTCTACCGGGTCCATACCAGTCGGGGTGTTAATGAAAACCTGAATGCACCAGGCA
TGCATTTTGTACCATTGGCATTTGATACCACCTGTATGCAGACCGATCCCTCCGCTCCTCCGGATGCAATCCCTAATCGT
TTTTATACCTATGGTGTAATCGCCAGGCTGGCACTACTTGCCTCTGCCATCGAGCTGGAACGGTATATTCAAACCCCATT
GTCCACACTGGCAATGGCCGAAACCCGATGA

Upstream 100 bases:

>100_bases
ACTCTTTCAAGTGTGCTTATTGCCTTTTTTATAGACAGAATCTGCCCATTCAGGTGCGGTGTTATAATCAGCCGGTTTAT
TTCCAAGTAATGGATCTCTC

Downstream 100 bases:

>100_bases
AACTGGCTTTTATTCTAGACCCGCTCGACAGTATCAAAATTGGCAAGGATTCAAGCTATGCCATGATGTGTGAAGCGGCG
TCCCGTCACCATCAGCTGTA

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPVPHLTNTLNAPTFDLEKRILTAKPTIEHWFRKQWQLHTVPFYCSVDLRNSGFKLAPVDTNLFPGGFNNLNPTFIPLCI
QAMMNAVEKICPDAHNVLLIPENHTRNIFYLQNIAMLQSIMQQAGMNIRIGSLLPGIDHPVSVPLPGGEPLLLEPVQRKG
NKLFVQDFDPCAILLNNDLSSGVPEILQNLDQTIIPPLHAGWATRRKSWHFSAYDNVAQDFAELIDIDPWLINPYFTACG
KVDFQEKEGLDCVAAAVDEILNQIREKYTQYGISEEPFVIVKADSGTYGMGIMSIKDGTEVYKLNRKQRNKMTTIKEGLV
VKNVLVQEGVHTFENIHQAVAEPVIYMIDHHVVGGFYRVHTSRGVNENLNAPGMHFVPLAFDTTCMQTDPSAPPDAIPNR
FYTYGVIARLALLASAIELERYIQTPLSTLAMAETR

Sequences:

>Translated_436_residues
MPVPHLTNTLNAPTFDLEKRILTAKPTIEHWFRKQWQLHTVPFYCSVDLRNSGFKLAPVDTNLFPGGFNNLNPTFIPLCI
QAMMNAVEKICPDAHNVLLIPENHTRNIFYLQNIAMLQSIMQQAGMNIRIGSLLPGIDHPVSVPLPGGEPLLLEPVQRKG
NKLFVQDFDPCAILLNNDLSSGVPEILQNLDQTIIPPLHAGWATRRKSWHFSAYDNVAQDFAELIDIDPWLINPYFTACG
KVDFQEKEGLDCVAAAVDEILNQIREKYTQYGISEEPFVIVKADSGTYGMGIMSIKDGTEVYKLNRKQRNKMTTIKEGLV
VKNVLVQEGVHTFENIHQAVAEPVIYMIDHHVVGGFYRVHTSRGVNENLNAPGMHFVPLAFDTTCMQTDPSAPPDAIPNR
FYTYGVIARLALLASAIELERYIQTPLSTLAMAETR
>Mature_435_residues
PVPHLTNTLNAPTFDLEKRILTAKPTIEHWFRKQWQLHTVPFYCSVDLRNSGFKLAPVDTNLFPGGFNNLNPTFIPLCIQ
AMMNAVEKICPDAHNVLLIPENHTRNIFYLQNIAMLQSIMQQAGMNIRIGSLLPGIDHPVSVPLPGGEPLLLEPVQRKGN
KLFVQDFDPCAILLNNDLSSGVPEILQNLDQTIIPPLHAGWATRRKSWHFSAYDNVAQDFAELIDIDPWLINPYFTACGK
VDFQEKEGLDCVAAAVDEILNQIREKYTQYGISEEPFVIVKADSGTYGMGIMSIKDGTEVYKLNRKQRNKMTTIKEGLVV
KNVLVQEGVHTFENIHQAVAEPVIYMIDHHVVGGFYRVHTSRGVNENLNAPGMHFVPLAFDTTCMQTDPSAPPDAIPNRF
YTYGVIARLALLASAIELERYIQTPLSTLAMAETR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48903; Mature: 48772

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPHLTNTLNAPTFDLEKRILTAKPTIEHWFRKQWQLHTVPFYCSVDLRNSGFKLAPVD
CCCCCCCCCCCCCCHHHHHHHEECCCHHHHHHHHCCEEEECCEEEEEEECCCCEEEEECC
TNLFPGGFNNLNPTFIPLCIQAMMNAVEKICPDAHNVLLIPENHTRNIFYLQNIAMLQSI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEHHHHHHHHH
MQQAGMNIRIGSLLPGIDHPVSVPLPGGEPLLLEPVQRKGNKLFVQDFDPCAILLNNDLS
HHHCCCCEEECCCCCCCCCCEECCCCCCCCEEECHHHHCCCEEEEECCCCEEEEECCCCC
SGVPEILQNLDQTIIPPLHAGWATRRKSWHFSAYDNVAQDFAELIDIDPWLINPYFTACG
CCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCC
KVDFQEKEGLDCVAAAVDEILNQIREKYTQYGISEEPFVIVKADSGTYGMGIMSIKDGTE
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCH
VYKLNRKQRNKMTTIKEGLVVKNVLVQEGVHTFENIHQAVAEPVIYMIDHHVVGGFYRVH
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
TSRGVNENLNAPGMHFVPLAFDTTCMQTDPSAPPDAIPNRFYTYGVIARLALLASAIELE
CCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYIQTPLSTLAMAETR
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVPHLTNTLNAPTFDLEKRILTAKPTIEHWFRKQWQLHTVPFYCSVDLRNSGFKLAPVD
CCCCCCCCCCCCCHHHHHHHEECCCHHHHHHHHCCEEEECCEEEEEEECCCCEEEEECC
TNLFPGGFNNLNPTFIPLCIQAMMNAVEKICPDAHNVLLIPENHTRNIFYLQNIAMLQSI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEHHHHHHHHH
MQQAGMNIRIGSLLPGIDHPVSVPLPGGEPLLLEPVQRKGNKLFVQDFDPCAILLNNDLS
HHHCCCCEEECCCCCCCCCCEECCCCCCCCEEECHHHHCCCEEEEECCCCEEEEECCCCC
SGVPEILQNLDQTIIPPLHAGWATRRKSWHFSAYDNVAQDFAELIDIDPWLINPYFTACG
CCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCC
KVDFQEKEGLDCVAAAVDEILNQIREKYTQYGISEEPFVIVKADSGTYGMGIMSIKDGTE
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCH
VYKLNRKQRNKMTTIKEGLVVKNVLVQEGVHTFENIHQAVAEPVIYMIDHHVVGGFYRVH
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
TSRGVNENLNAPGMHFVPLAFDTTCMQTDPSAPPDAIPNRFYTYGVIARLALLASAIELE
CCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYIQTPLSTLAMAETR
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA