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The map label for this gene is gshB [H]

Identifier: 114330956

GI number: 114330956

Start: 983993

End: 984943

Strand: Reverse

Name: gshB [H]

Synonym: Neut_0953

Alternate gene names: 114330956

Gene position: 984943-983993 (Counterclockwise)

Preceding gene: 114330957

Following gene: 114330954

Centisome position: 37.01

GC content: 47.63

Gene sequence:

>951_bases
ATGAAACTGGCTTTTATTCTAGACCCGCTCGACAGTATCAAAATTGGCAAGGATTCAAGCTATGCCATGATGTGTGAAGC
GGCGTCCCGTCACCATCAGCTGTATACCTTGCAACAAAGTGGCTTGGCCTGGAAAAATCACCAGGTAATTGGTTTCGCAC
GATCACTGACACTCATCGATCCTCCGGATAACCCCCATCGCTGGTATGAGGAAGGCGTTATAGAAGAAATTCCACTGACC
GAATTTGATGCCGTGCTCATGCGCAAGGATCCTCCCTTCGACACCGAGTATATCTACAGCACCTATCTACTCGAACTGGC
TGAACGTCAGGGTGCTTACGTTGTGAACAGCCCACGGGGCATTCGTGATCACAATGAGAAACTGGCAATCACAGAATTTC
CACAATTTATACCCTCTTCACTGGTAACCAGCCAGGAACAGCTGATTCTCCAGTTTCTTGCCGAACATAAGGATATTATC
CTGAAACCACTGGATGGCATGGGAGGTGCTGGTATTTTTCGCATGCAGAACACCGACCATAATATTGGCGTCATCATCGA
AACGCTAACACGTTATGGCACTCGTACTATCATGGCACAACGCTTTCTACCGGAAATCCGCGAAGGAGACAAACGTATAT
TGCTAATTGCTGGAAAACCTGTCGATTATGCACTCGCACGCATCCCCAAACCGGGAGAAACGCGTGGCAATCTGGCGGTT
GGTGGCACCGGTAAAGTACAACCGCTGTCAGCACGTGACAGGAAAATTTCTGAAGAACTCGGACAAATCCTTTATGCTCG
CGGCTTGATGCTGATAGGCTTGGATGTCATCGGCAACCATCTAACGGAAATAAATGTCACCAGTCCAACCGGTATGCGGG
AAATCTCTGATCAAACAGGAATAAATGTTGCAGGGCTAATGATTGATGCGCTGGAACAAAATATTCATTGA

Upstream 100 bases:

>100_bases
CCTATGGTGTAATCGCCAGGCTGGCACTACTTGCCTCTGCCATCGAGCTGGAACGGTATATTCAAACCCCATTGTCCACA
CTGGCAATGGCCGAAACCCG

Downstream 100 bases:

>100_bases
AATACTAAAGAAGTGGCTAAAAAAGGCCTGATTAACAGGCCTTTTTTATGTAAACAAAACGGATCAATAACCGCCTTTCC
AGGCGTCACCCGATTTTTCT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKLAFILDPLDSIKIGKDSSYAMMCEAASRHHQLYTLQQSGLAWKNHQVIGFARSLTLIDPPDNPHRWYEEGVIEEIPLT
EFDAVLMRKDPPFDTEYIYSTYLLELAERQGAYVVNSPRGIRDHNEKLAITEFPQFIPSSLVTSQEQLILQFLAEHKDII
LKPLDGMGGAGIFRMQNTDHNIGVIIETLTRYGTRTIMAQRFLPEIREGDKRILLIAGKPVDYALARIPKPGETRGNLAV
GGTGKVQPLSARDRKISEELGQILYARGLMLIGLDVIGNHLTEINVTSPTGMREISDQTGINVAGLMIDALEQNIH

Sequences:

>Translated_316_residues
MKLAFILDPLDSIKIGKDSSYAMMCEAASRHHQLYTLQQSGLAWKNHQVIGFARSLTLIDPPDNPHRWYEEGVIEEIPLT
EFDAVLMRKDPPFDTEYIYSTYLLELAERQGAYVVNSPRGIRDHNEKLAITEFPQFIPSSLVTSQEQLILQFLAEHKDII
LKPLDGMGGAGIFRMQNTDHNIGVIIETLTRYGTRTIMAQRFLPEIREGDKRILLIAGKPVDYALARIPKPGETRGNLAV
GGTGKVQPLSARDRKISEELGQILYARGLMLIGLDVIGNHLTEINVTSPTGMREISDQTGINVAGLMIDALEQNIH
>Mature_316_residues
MKLAFILDPLDSIKIGKDSSYAMMCEAASRHHQLYTLQQSGLAWKNHQVIGFARSLTLIDPPDNPHRWYEEGVIEEIPLT
EFDAVLMRKDPPFDTEYIYSTYLLELAERQGAYVVNSPRGIRDHNEKLAITEFPQFIPSSLVTSQEQLILQFLAEHKDII
LKPLDGMGGAGIFRMQNTDHNIGVIIETLTRYGTRTIMAQRFLPEIREGDKRILLIAGKPVDYALARIPKPGETRGNLAV
GGTGKVQPLSARDRKISEELGQILYARGLMLIGLDVIGNHLTEINVTSPTGMREISDQTGINVAGLMIDALEQNIH

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=316, Percent_Identity=47.7848101265823, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35343; Mature: 35343

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAFILDPLDSIKIGKDSSYAMMCEAASRHHQLYTLQQSGLAWKNHQVIGFARSLTLID
CEEEEEECCCCCEECCCCCCCEEEEHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEEEC
PPDNPHRWYEEGVIEEIPLTEFDAVLMRKDPPFDTEYIYSTYLLELAERQGAYVVNSPRG
CCCCCHHHHHCCCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
IRDHNEKLAITEFPQFIPSSLVTSQEQLILQFLAEHKDIILKPLDGMGGAGIFRMQNTDH
CCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCC
NIGVIIETLTRYGTRTIMAQRFLPEIREGDKRILLIAGKPVDYALARIPKPGETRGNLAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEE
GGTGKVQPLSARDRKISEELGQILYARGLMLIGLDVIGNHLTEINVTSPTGMREISDQTG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCEEEEEECCCCCHHHHHHCCC
INVAGLMIDALEQNIH
CCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLAFILDPLDSIKIGKDSSYAMMCEAASRHHQLYTLQQSGLAWKNHQVIGFARSLTLID
CEEEEEECCCCCEECCCCCCCEEEEHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEEEC
PPDNPHRWYEEGVIEEIPLTEFDAVLMRKDPPFDTEYIYSTYLLELAERQGAYVVNSPRG
CCCCCHHHHHCCCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
IRDHNEKLAITEFPQFIPSSLVTSQEQLILQFLAEHKDIILKPLDGMGGAGIFRMQNTDH
CCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCC
NIGVIIETLTRYGTRTIMAQRFLPEIREGDKRILLIAGKPVDYALARIPKPGETRGNLAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEE
GGTGKVQPLSARDRKISEELGQILYARGLMLIGLDVIGNHLTEINVTSPTGMREISDQTG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCEEEEEECCCCCHHHHHHCCC
INVAGLMIDALEQNIH
CCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]