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The map label for this gene is ligA [H]

Identifier: 114330949

GI number: 114330949

Start: 976793

End: 978940

Strand: Reverse

Name: ligA [H]

Synonym: Neut_0946

Alternate gene names: 114330949

Gene position: 978940-976793 (Counterclockwise)

Preceding gene: 114330951

Following gene: 114330948

Centisome position: 36.79

GC content: 48.65

Gene sequence:

>2148_bases
ATGAAAAAACCCGGAAACTCTCTCCGCGCAATCTATTTCTATTATCCTACAACCGGGATTTTCCTATTCTACTCCCCTCA
CATGGTAATTCTTGACATGGAATCCGAAAAAATAATTGAAGAACGCCTGAAAGCATTACGTTCAACCATTGCGTTGCATG
ACTTTCATTACTACGTGCAGGATGCGCCAATTATTCCAGATGCAGATTACGACAAGCTTTTTCGCACCTTGCAACAACTG
GAACAGCAATATCCGCATCTGATCACACCGGATTCACCTACCCAGCGGGTGGGTGCTCCCCCGCTAAAAGTATTTGCTCG
GCTCACTCACCAGACACCCATGTTGTCACTGACCAATGCTTTTACAGAAGATGAGGCCATTGCCTTTGATAAGCGCATTC
GTGAAGCGCTGAATGTGGATCAGGTTAACTACGCAGTTGAACCCAAATTTGATGGACTGGCTGTCAGCCTGGTTTATACA
AATGGAATATTGACAAACGGAGCCACCCGGGGAGATGGTTATACCGGGGAAGATATCACACTGAATCTGCGCACAATTCC
ATCCATACCCTTGCGCCTGCAGACAGCCACTGTCACTGATCAGTTCGAGGTACGTGGCGAAGTGGTTATGTTGAAAGCAG
ACTTTGAGCATCTCAATGAACAACAGCGAAACAAGGGGGAGAAGCTCTTTGCCAATCCACGTAATGCCGCTGCCGGCTCG
CTACGTCAGTTGGATTCCAATATCACCGCTGCACGCAAACTGACTTTCTTTGCTTATGATGCCGTGCTCTCTCATAAAGA
TCAGCCGTTTTTTTCCAAACATAGCGAAATACTAGATTATCTTAAATCACAACAGTTTCTGGTGGCGCAGCAAAATGGTA
CGACAACGGGCGTGGCTGGACTATTAACCTATTACCATGAAATGGGTGCCTTACGCTTATCCCTTCCCTATGAAATTGAC
GGTGTGGTGTACAAAGTCGATAATCTGACGCAACAGGAAACACTTGGTTATGTCTCACGTGCTCCCCGTTTTGCTATTGC
ACATAAATTCCCGGCGCAAGAAGCCAGTACCGAATTACTGACAATCGAAATCCAGGTGGGAAGAACCGGTGCCCTGACGC
CGGTTGCACGCCTGGCCCCGGTATTTGTGGGGGGAGTAACAGTTACCAATGCCACTCTGCACAACGAAGATGAAGTTCAA
CGCAAACAGATCATGATCGGCGATACCGTCATCGTGCGCCGGGCGGGCGATGTCATCCCGGAAGTGGTGGCGGTAATACC
GGAACAGCGCCCTGCGCACGCCCAACCGTTCATCATGCCGGATCATTGCCCGGTATGCGGATCAAAAGCGGCACGTCTAC
CAGGTGAAGCAGTGACCCGCTGTACCGGAGGTTTATATTGTCCAGCTCAGCGTAAACAGGCAATCTGGCATTTTGCTTCC
CGCCGTGCCCTTGATATTGATGGATTGGGGGAAAAACTGATTGATCAATTAATTGACCGCGAACTGGTGCATACCCCTGC
CGATCTGTATAAACTGAACATTGATACGCTCGCGAGCCTGGAACGCATGGCTGAAAAATCCGCACGCAACCTCGTTACCG
CAATTGAACACAGCAAAAAAACTACGTTGCCGCGTTTTATTTATGCGCTGGGTATTCGTCATGTTGGTGAAGCGACTGCC
AAGGCGCTTGCTAACCAGGCAAGCCATCTCGATCAGCTGATGACTCTGGATATTGAACAGTTACAACAAATCCCGGATGT
CGGCCCGATAGTTGCGCAAAGTATTATAGATTTTTTTTCTGAAGCACATAACCGGGAAGTGATCAGACAACTGCTTGATA
GCGGGTTGCAGTGGGAAATACCAAACCATGGCGTACAACAATCAGAACAAACCAATTCAGCTGTTTCCGGTAAAACCTTC
GTTCTGACCGGAACTTTGCCAACCATGACGCGCGATCAGGCCAAAATCAAAATTGAACAACAAGGCGGCAAGGTAACCGG
CAGCGTTTCATCTGCCACCAGTTATGTCGTTGCGGGATCAGATCCCGGCAGCAAGTACGCTAAAGCAATCGGGTTAGGCA
TTTCTGTACTGGATGAAGATCAGCTACTGTCACTGCTGCAAAATATCTCGACAGGTGCACAACAGTAA

Upstream 100 bases:

>100_bases
CCGATGAGGATTGATAATCCGGTCAATCTGATATTCATATTACTTCTCCTGTTGTAAATAAAATTACAAATATTTAGACA
ATCTGTCTTGCGCAAAGTTT

Downstream 100 bases:

>100_bases
GGTAATTGATCAATAACAATCTTCTTGATTGCAGTTATCTTCTCTTGCACGCGTATTACGCGTACGTAGCCAGTTATAAT
ATAAGTGTAAAAAAATAACT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MKKPGNSLRAIYFYYPTTGIFLFYSPHMVILDMESEKIIEERLKALRSTIALHDFHYYVQDAPIIPDADYDKLFRTLQQL
EQQYPHLITPDSPTQRVGAPPLKVFARLTHQTPMLSLTNAFTEDEAIAFDKRIREALNVDQVNYAVEPKFDGLAVSLVYT
NGILTNGATRGDGYTGEDITLNLRTIPSIPLRLQTATVTDQFEVRGEVVMLKADFEHLNEQQRNKGEKLFANPRNAAAGS
LRQLDSNITAARKLTFFAYDAVLSHKDQPFFSKHSEILDYLKSQQFLVAQQNGTTTGVAGLLTYYHEMGALRLSLPYEID
GVVYKVDNLTQQETLGYVSRAPRFAIAHKFPAQEASTELLTIEIQVGRTGALTPVARLAPVFVGGVTVTNATLHNEDEVQ
RKQIMIGDTVIVRRAGDVIPEVVAVIPEQRPAHAQPFIMPDHCPVCGSKAARLPGEAVTRCTGGLYCPAQRKQAIWHFAS
RRALDIDGLGEKLIDQLIDRELVHTPADLYKLNIDTLASLERMAEKSARNLVTAIEHSKKTTLPRFIYALGIRHVGEATA
KALANQASHLDQLMTLDIEQLQQIPDVGPIVAQSIIDFFSEAHNREVIRQLLDSGLQWEIPNHGVQQSEQTNSAVSGKTF
VLTGTLPTMTRDQAKIKIEQQGGKVTGSVSSATSYVVAGSDPGSKYAKAIGLGISVLDEDQLLSLLQNISTGAQQ

Sequences:

>Translated_715_residues
MKKPGNSLRAIYFYYPTTGIFLFYSPHMVILDMESEKIIEERLKALRSTIALHDFHYYVQDAPIIPDADYDKLFRTLQQL
EQQYPHLITPDSPTQRVGAPPLKVFARLTHQTPMLSLTNAFTEDEAIAFDKRIREALNVDQVNYAVEPKFDGLAVSLVYT
NGILTNGATRGDGYTGEDITLNLRTIPSIPLRLQTATVTDQFEVRGEVVMLKADFEHLNEQQRNKGEKLFANPRNAAAGS
LRQLDSNITAARKLTFFAYDAVLSHKDQPFFSKHSEILDYLKSQQFLVAQQNGTTTGVAGLLTYYHEMGALRLSLPYEID
GVVYKVDNLTQQETLGYVSRAPRFAIAHKFPAQEASTELLTIEIQVGRTGALTPVARLAPVFVGGVTVTNATLHNEDEVQ
RKQIMIGDTVIVRRAGDVIPEVVAVIPEQRPAHAQPFIMPDHCPVCGSKAARLPGEAVTRCTGGLYCPAQRKQAIWHFAS
RRALDIDGLGEKLIDQLIDRELVHTPADLYKLNIDTLASLERMAEKSARNLVTAIEHSKKTTLPRFIYALGIRHVGEATA
KALANQASHLDQLMTLDIEQLQQIPDVGPIVAQSIIDFFSEAHNREVIRQLLDSGLQWEIPNHGVQQSEQTNSAVSGKTF
VLTGTLPTMTRDQAKIKIEQQGGKVTGSVSSATSYVVAGSDPGSKYAKAIGLGISVLDEDQLLSLLQNISTGAQQ
>Mature_715_residues
MKKPGNSLRAIYFYYPTTGIFLFYSPHMVILDMESEKIIEERLKALRSTIALHDFHYYVQDAPIIPDADYDKLFRTLQQL
EQQYPHLITPDSPTQRVGAPPLKVFARLTHQTPMLSLTNAFTEDEAIAFDKRIREALNVDQVNYAVEPKFDGLAVSLVYT
NGILTNGATRGDGYTGEDITLNLRTIPSIPLRLQTATVTDQFEVRGEVVMLKADFEHLNEQQRNKGEKLFANPRNAAAGS
LRQLDSNITAARKLTFFAYDAVLSHKDQPFFSKHSEILDYLKSQQFLVAQQNGTTTGVAGLLTYYHEMGALRLSLPYEID
GVVYKVDNLTQQETLGYVSRAPRFAIAHKFPAQEASTELLTIEIQVGRTGALTPVARLAPVFVGGVTVTNATLHNEDEVQ
RKQIMIGDTVIVRRAGDVIPEVVAVIPEQRPAHAQPFIMPDHCPVCGSKAARLPGEAVTRCTGGLYCPAQRKQAIWHFAS
RRALDIDGLGEKLIDQLIDRELVHTPADLYKLNIDTLASLERMAEKSARNLVTAIEHSKKTTLPRFIYALGIRHVGEATA
KALANQASHLDQLMTLDIEQLQQIPDVGPIVAQSIIDFFSEAHNREVIRQLLDSGLQWEIPNHGVQQSEQTNSAVSGKTF
VLTGTLPTMTRDQAKIKIEQQGGKVTGSVSSATSYVVAGSDPGSKYAKAIGLGISVLDEDQLLSLLQNISTGAQQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=78, Percent_Identity=48.7179487179487, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=53.4023668639053, Blast_Score=712, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 79064; Mature: 79064

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPGNSLRAIYFYYPTTGIFLFYSPHMVILDMESEKIIEERLKALRSTIALHDFHYYVQ
CCCCCCCEEEEEEEECCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEC
DAPIIPDADYDKLFRTLQQLEQQYPHLITPDSPTQRVGAPPLKVFARLTHQTPMLSLTNA
CCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHC
FTEDEAIAFDKRIREALNVDQVNYAVEPKFDGLAVSLVYTNGILTNGATRGDGYTGEDIT
CCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCEEECCCCCCCCCCCCCEE
LNLRTIPSIPLRLQTATVTDQFEVRGEVVMLKADFEHLNEQQRNKGEKLFANPRNAAAGS
EEEEECCCCCEEEEEEEECCCEEECCCEEEEECCHHHHHHHHHCCCCEEECCCCCHHHHH
LRQLDSNITAARKLTFFAYDAVLSHKDQPFFSKHSEILDYLKSQQFLVAQQNGTTTGVAG
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
LLTYYHEMGALRLSLPYEIDGVVYKVDNLTQQETLGYVSRAPRFAIAHKFPAQEASTELL
HHHHHHHHCCEEEECCEEECCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEE
TIEIQVGRTGALTPVARLAPVFVGGVTVTNATLHNEDEVQRKQIMIGDTVIVRRAGDVIP
EEEEEECCCCCCCHHHHHHHHHCCCEEEEECEECCHHHHHHHHEEECCEEEEEHHHHHHH
EVVAVIPEQRPAHAQPFIMPDHCPVCGSKAARLPGEAVTRCTGGLYCPAQRKQAIWHFAS
HHHHHCCCCCCCCCCCEECCCCCCCCCCCHHCCCHHHHHHHCCCEECCHHHHHHHHHHHC
RRALDIDGLGEKLIDQLIDRELVHTPADLYKLNIDTLASLERMAEKSARNLVTAIEHSKK
CCEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTLPRFIYALGIRHVGEATAKALANQASHLDQLMTLDIEQLQQIPDVGPIVAQSIIDFFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHH
EAHNREVIRQLLDSGLQWEIPNHGVQQSEQTNSAVSGKTFVLTGTLPTMTRDQAKIKIEQ
HHCCHHHHHHHHHCCCEEECCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEE
QGGKVTGSVSSATSYVVAGSDPGSKYAKAIGLGISVLDEDQLLSLLQNISTGAQQ
CCCEEEEECCCCCEEEEECCCCCHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKPGNSLRAIYFYYPTTGIFLFYSPHMVILDMESEKIIEERLKALRSTIALHDFHYYVQ
CCCCCCCEEEEEEEECCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEC
DAPIIPDADYDKLFRTLQQLEQQYPHLITPDSPTQRVGAPPLKVFARLTHQTPMLSLTNA
CCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHC
FTEDEAIAFDKRIREALNVDQVNYAVEPKFDGLAVSLVYTNGILTNGATRGDGYTGEDIT
CCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCEEECCCCCCCCCCCCCEE
LNLRTIPSIPLRLQTATVTDQFEVRGEVVMLKADFEHLNEQQRNKGEKLFANPRNAAAGS
EEEEECCCCCEEEEEEEECCCEEECCCEEEEECCHHHHHHHHHCCCCEEECCCCCHHHHH
LRQLDSNITAARKLTFFAYDAVLSHKDQPFFSKHSEILDYLKSQQFLVAQQNGTTTGVAG
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
LLTYYHEMGALRLSLPYEIDGVVYKVDNLTQQETLGYVSRAPRFAIAHKFPAQEASTELL
HHHHHHHHCCEEEECCEEECCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEE
TIEIQVGRTGALTPVARLAPVFVGGVTVTNATLHNEDEVQRKQIMIGDTVIVRRAGDVIP
EEEEEECCCCCCCHHHHHHHHHCCCEEEEECEECCHHHHHHHHEEECCEEEEEHHHHHHH
EVVAVIPEQRPAHAQPFIMPDHCPVCGSKAARLPGEAVTRCTGGLYCPAQRKQAIWHFAS
HHHHHCCCCCCCCCCCEECCCCCCCCCCCHHCCCHHHHHHHCCCEECCHHHHHHHHHHHC
RRALDIDGLGEKLIDQLIDRELVHTPADLYKLNIDTLASLERMAEKSARNLVTAIEHSKK
CCEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTLPRFIYALGIRHVGEATAKALANQASHLDQLMTLDIEQLQQIPDVGPIVAQSIIDFFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHH
EAHNREVIRQLLDSGLQWEIPNHGVQQSEQTNSAVSGKTFVLTGTLPTMTRDQAKIKIEQ
HHCCHHHHHHHHHCCCEEECCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEE
QGGKVTGSVSSATSYVVAGSDPGSKYAKAIGLGISVLDEDQLLSLLQNISTGAQQ
CCCEEEEECCCCCEEEEECCCCCHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA