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The map label for this gene is nuoF2 [H]

Identifier: 114330930

GI number: 114330930

Start: 951976

End: 953253

Strand: Direct

Name: nuoF2 [H]

Synonym: Neut_0927

Alternate gene names: 114330930

Gene position: 951976-953253 (Clockwise)

Preceding gene: 114330929

Following gene: 114330931

Centisome position: 35.77

GC content: 45.23

Gene sequence:

>1278_bases
ATGACTCAACCTACTCAAGTCATCATGGCGGGTGTCGATTCTGCAGATCCAAATAATTGGCGGTTAAAAAGTTATTTAAA
ACGTGACGGATATGTTGCGTTAAAAAAAATTCTTTCGCAAAAAATTCCTCCGGAAGTTGTGATTGAGGAAGTGAAGAAAT
CCGCACTACGTGGGCGCGGTGGTGCTGGTTTTCCTACTGGCTTGAAGTGGAGTTTTATGCCAAAACAGTATACAGGTGAG
AAATATCTTGTCTGTAACTCTGATGAAGGAGAGCCGGGCACTTTCAAGGATCGCGATATCATGCGTTACAATCCACATGT
CCTTATTGAAGGTATGTTGATTGCTGCCTATGCGATGGGTATCCGCACCGGATATAACTATGTGCACGGTGAAATCTGGG
ATGTTTACGAACGAATGGAGGAGGCTGTCGAGGAAGCCAATGCGGCAGGATTTCTCGGGGAGAATATTCTGGATTCTGGG
TTCGTTTTTCAGCTCTACAATCATCATGGATATGGCGCCTATATATGTGGAGAAGAAACCGCGCTATTAGAATCGATAGA
AGGTAAAAAGGGACAGCCACGCTTTAAACCTCCGTTTCCGGCTAATTTTGGCTTATACGGCAAGCCAACTACGATCAACA
ATACAGAAACGTTTGCTTCAGTTCCTTGGATTATGCGTAACGGGGGCGAAAGTTACTTGCAGCTGGGAAAACCGAATAAT
GGAGGTACCAAGATATTTTCGGTTTCCGGTCATGTAAATAAACCGGGTAACTATGAAGTGCCTATGGGCACTCCGTTTTC
AAAGTTGCTGGAACTTGCCGGCGGAATGCGCGGGGGGCGGAAATTGAAAGGATGTATCCCCGGTGGTTCATCCATGCCAG
TTTTACCTGGCAGCATCATGATGGAAACAGACATGGATTATGATTCAATAGCAAAAGCAGGCTCCATGTTGGGTTCGGGG
GCGGTCATTATTATGGACGATACGACTTGCATGGTGCGTGCACTAGAACGCCTTTCCTATTTCTATTATGAGGAATCTTG
TGGCCAATGTACTCCATGCCGAGAAGGAACAGGGTGGCTTTACAGGATCATCAATCGGATTGAGCATGGTAAAGGAAGGA
TTGAAGATCTGGATGTACTCAACAATCTTGCAGATAATATCCAGGGAAGAACTATCTGCGCGTTAGGTGATGCTGCTGCG
ATGCCAGTACGAGCTATGTTGCATCACTTCTGGGATGAATTTGTTTTTCATATTGAGCATAAAAAGTGCATGGTCTAG

Upstream 100 bases:

>100_bases
GAGCTTGTGGAGATGCGCCAGTACTTCTGGTTAATAACAAGCGTATGTGCAGCTTTATGACTGAGGATCAAATAGACAAA
TTGCTTGAGGAATTAAAGAG

Downstream 100 bases:

>100_bases
GTGAACCATGGTTTTAGTGCTGTATTTATCCAACTCCCGATAATCTTGGGAATTATTGCTGATGATTAATATTGAAATTG
ATGGCAAGCAAGTAGCCGTT

Product: NADH-quinone oxidoreductase, F subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTQPTQVIMAGVDSADPNNWRLKSYLKRDGYVALKKILSQKIPPEVVIEEVKKSALRGRGGAGFPTGLKWSFMPKQYTGE
KYLVCNSDEGEPGTFKDRDIMRYNPHVLIEGMLIAAYAMGIRTGYNYVHGEIWDVYERMEEAVEEANAAGFLGENILDSG
FVFQLYNHHGYGAYICGEETALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETFASVPWIMRNGGESYLQLGKPNN
GGTKIFSVSGHVNKPGNYEVPMGTPFSKLLELAGGMRGGRKLKGCIPGGSSMPVLPGSIMMETDMDYDSIAKAGSMLGSG
AVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWLYRIINRIEHGKGRIEDLDVLNNLADNIQGRTICALGDAAA
MPVRAMLHHFWDEFVFHIEHKKCMV

Sequences:

>Translated_425_residues
MTQPTQVIMAGVDSADPNNWRLKSYLKRDGYVALKKILSQKIPPEVVIEEVKKSALRGRGGAGFPTGLKWSFMPKQYTGE
KYLVCNSDEGEPGTFKDRDIMRYNPHVLIEGMLIAAYAMGIRTGYNYVHGEIWDVYERMEEAVEEANAAGFLGENILDSG
FVFQLYNHHGYGAYICGEETALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETFASVPWIMRNGGESYLQLGKPNN
GGTKIFSVSGHVNKPGNYEVPMGTPFSKLLELAGGMRGGRKLKGCIPGGSSMPVLPGSIMMETDMDYDSIAKAGSMLGSG
AVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWLYRIINRIEHGKGRIEDLDVLNNLADNIQGRTICALGDAAA
MPVRAMLHHFWDEFVFHIEHKKCMV
>Mature_424_residues
TQPTQVIMAGVDSADPNNWRLKSYLKRDGYVALKKILSQKIPPEVVIEEVKKSALRGRGGAGFPTGLKWSFMPKQYTGEK
YLVCNSDEGEPGTFKDRDIMRYNPHVLIEGMLIAAYAMGIRTGYNYVHGEIWDVYERMEEAVEEANAAGFLGENILDSGF
VFQLYNHHGYGAYICGEETALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETFASVPWIMRNGGESYLQLGKPNNG
GTKIFSVSGHVNKPGNYEVPMGTPFSKLLELAGGMRGGRKLKGCIPGGSSMPVLPGSIMMETDMDYDSIAKAGSMLGSGA
VIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWLYRIINRIEHGKGRIEDLDVLNNLADNIQGRTICALGDAAAM
PVRAMLHHFWDEFVFHIEHKKCMV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=400, Percent_Identity=49.75, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI260656005, Length=400, Percent_Identity=49.75, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1788620, Length=416, Percent_Identity=42.7884615384615, Blast_Score=342, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17531727, Length=400, Percent_Identity=49, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI19920794, Length=400, Percent_Identity=49, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45550452, Length=396, Percent_Identity=48.4848484848485, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24653819, Length=403, Percent_Identity=37.468982630273, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24653817, Length=403, Percent_Identity=33.7468982630273, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47114; Mature: 46982

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPTQVIMAGVDSADPNNWRLKSYLKRDGYVALKKILSQKIPPEVVIEEVKKSALRGRG
CCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GAGFPTGLKWSFMPKQYTGEKYLVCNSDEGEPGTFKDRDIMRYNPHVLIEGMLIAAYAMG
CCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHH
IRTGYNYVHGEIWDVYERMEEAVEEANAAGFLGENILDSGFVFQLYNHHGYGAYICGEET
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCEEEEECCHH
ALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETFASVPWIMRNGGESYLQLGKPNN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCHHHEEECCCCC
GGTKIFSVSGHVNKPGNYEVPMGTPFSKLLELAGGMRGGRKLKGCIPGGSSMPVLPGSIM
CCCEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEE
METDMDYDSIAKAGSMLGSGAVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWL
EECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
YRIINRIEHGKGRIEDLDVLNNLADNIQGRTICALGDAAAMPVRAMLHHFWDEFVFHIEH
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEECC
KKCMV
HHCCC
>Mature Secondary Structure 
TQPTQVIMAGVDSADPNNWRLKSYLKRDGYVALKKILSQKIPPEVVIEEVKKSALRGRG
CCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GAGFPTGLKWSFMPKQYTGEKYLVCNSDEGEPGTFKDRDIMRYNPHVLIEGMLIAAYAMG
CCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHH
IRTGYNYVHGEIWDVYERMEEAVEEANAAGFLGENILDSGFVFQLYNHHGYGAYICGEET
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCEEEEECCHH
ALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETFASVPWIMRNGGESYLQLGKPNN
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCHHHEEECCCCC
GGTKIFSVSGHVNKPGNYEVPMGTPFSKLLELAGGMRGGRKLKGCIPGGSSMPVLPGSIM
CCCEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEE
METDMDYDSIAKAGSMLGSGAVIIMDDTTCMVRALERLSYFYYEESCGQCTPCREGTGWL
EECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
YRIINRIEHGKGRIEDLDVLNNLADNIQGRTICALGDAAAMPVRAMLHHFWDEFVFHIEH
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEECC
KKCMV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]